2006
DOI: 10.1002/jssc.200500423
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Trace analysis of antibacterial tylosin A, B, C and D in honey by liquid chromatography-electrospray ionization-mass spectrometry

Abstract: A new LC-ESI-MS method was developed for the determination of residues of the antibacterial tylosins A, B, C and D in honey. The procedure employed an SPE on polymeric cartridges for the isolation of tylosins from diluted honey. Chromatographic separation of the tylosins was performed on a C18 column (150 x 4.60 mm2 ID, 5 microm) using a ternary gradient made of formic acid 1% in water (solvent A), methanol (solvent B) and ACN (solvent C) as mobile phase, at 30 degrees C and at a flow rate of 0.8 mL/min. Avera… Show more

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Cited by 15 publications
(5 citation statements)
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“…Both cartridges were washed with water in order to remove sugars, and then the compounds were eluted as was indicated in the Materials and Methods section. In this sense, it must be indicated that the combination of methanol and acetonitrile as well as the addition of NH 4 OH allow the quantitative extraction of macrolides (18) and sulfonamides (12), respectively.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Both cartridges were washed with water in order to remove sugars, and then the compounds were eluted as was indicated in the Materials and Methods section. In this sense, it must be indicated that the combination of methanol and acetonitrile as well as the addition of NH 4 OH allow the quantitative extraction of macrolides (18) and sulfonamides (12), respectively.…”
Section: Resultsmentioning
confidence: 99%
“…Although immunoassay methods have been proposed for the determination of antibiotics in honey , liquid chromatography (LC) has been mainly used for the separation of these type of compounds, coupled to ultraviolet , and fluorimetric detection , , although mass spectrometric detection using single (MS) , , triple quadrupole (MS/MS) ,,,, , or time-of-flight (TOF) analysers provides more reliable determination. However, most of the developed analytical methods are multiresidue analyses within a single class of compound ,, , and very few multiclass methods for the determination of antibiotics in honey have been proposed , .…”
Section: Introductionmentioning
confidence: 99%
“…In addition, Canada authorities fixed an MRL in honey equal to 200 μg/kg as sum of tylosin A and B ( Table 1). The most significantly published procedures are summarized in Table 4 [31][32][33][34][35][36][37]. Lincomycin belongs to the group of lincosamides, and its activity against Paenibacillus larvae strains has been reported.…”
Section: Macrolides (Macs) and Lincomycinmentioning
confidence: 99%
“…The procedure described by Nozal-Nalda et al (2006) was modified to determine concentrations of TYL and its metabolites in manure liquid and solid samples. In brief, the analytes were separated a C18 column using formic acid 1% in water (solvent A), methanol (solvent B) and ACN (solvent C as mobile phase at a flow rate of 0.8 mL/min.…”
Section: Chromatography -Tylosin and Metabolitesmentioning
confidence: 99%