Trace detection of SARS-CoV-2 N-protein by diamond solution-gate field-effect transistor

Zhang, Qianwen; Zhang, Minghui; Du, Yuxiang; Xu, Bangqiang; Chen, Genqiang; He, Shi; Zhang, Dan; Li, Qi; Wang, Hongxing

doi:10.1016/j.diamond.2023.109775

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…The linear regression fit (red line) clearly shows that the higher the structure, the higher the SE/BSE intensity. Such dependence is similar to antibodies [12,13], but opposite to bacteria [14].…”

Section: Thiorphan Assembly On the Polar O-face Zno Surfacementioning

confidence: 68%

“…This is opposite to the observation on the polar ZnO surface (Figure 2). Such dependence is similar to bacteria [14], but it is opposite to antibodies [12,13].…”

Section: Thiorphan Assembly On the Nonpolar (101 ̅ 0) Zno Surfacementioning

confidence: 78%

“…Prior studies showed that antibody molecular assemblies can increase secondary electron (SE) intensity [12,13] whereas bacterial clusters showed decrease in SE intensity [14]. Similarly, SE intensity was used for measuring thickness of nanometer-thin SiO2 layers on silicon substrates [15].…”

Section: Introductionmentioning

confidence: 99%

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AFM-IN-SEM analyses of thiorphan assembly on ZnO polar and nonpolar surfaces

UKRAINTSEV,

HEMATIAN,

NEUMAN

et al. 2023

NANOCON Conference Proeedings

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Despite the importance of thiorphan as a small molecule with vital biological roles, its interactions with zinc oxide (ZnO) nanomaterials that are prospective in drug delivery and theranostic applications have not yet been sufficiently explored. Here the impact of surface polarity of different ZnO surfaces on thiorphan adsorption is studied experimentally by combined in-situ scanning electron microscope (SEM) and atomic force microscope (AFM). Polar ZnO surfaces cause formation of thiorphan nanodots ( 4 nm or 25 nm), where the size of the nanodots depends on the direction of the surface dipoles. Nonpolar ZnO surfaces cause self-assembly of thiorphan into nanoislands and nanolayers with characteristic 4 nm layer thickness. AFM-in-SEM data shows clear correlation between secondary electron intensity of molecules in SEM and their height in AFM on polar ZnO surface whereas anti-correlation is observed on nonpolar ZnO surface. The secondary electron emission from the same molecule thus depends on its orientation, structure it assembles into and it can be controlled by direction of the substrate surface dipole.

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Section: Thiorphan Assembly On the Polar O-face Zno Surfacementioning

confidence: 68%

“…This is opposite to the observation on the polar ZnO surface (Figure 2). Such dependence is similar to bacteria [14], but it is opposite to antibodies [12,13].…”

Section: Thiorphan Assembly On the Nonpolar (101 ̅ 0) Zno Surfacementioning

confidence: 78%

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AFM-IN-SEM analyses of thiorphan assembly on ZnO polar and nonpolar surfaces

UKRAINTSEV,

HEMATIAN,

NEUMAN

et al. 2023

NANOCON Conference Proeedings

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A biosensor based on magnetoelastic waves for detection of antibodies in human plasma for COVID-19 serodiagnosis

F. Silva,

P. Monteiro,

Senra

et al. 2024

Biosensors and Bioelectronics

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Optimization of diamond sensor for trace detection of SARS-CoV-2 N-protein using Au nanoparticles

Zhang,

Wu,

et al. 2023

Applied Physics Letters

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In this study, Au nanoparticles were introduced to functionalize hydrogen-terminated diamond (H-diamond) to optimize surface modification and improve the performance of diamond sensor for trace detection of SARS-CoV-2 N-protein. The Au nanoparticles were obtained by an electron beam evaporation system and annealing. Atom force microscope and scanning electron microscope were utilized to characterize the structure, morphology, and distribution of fabricated Au nanoparticles. The concentration of SARS-CoV-2 N-protein was determined by the investigation of transfer characteristics of a diamond solution gate field effect transistor. Sensitivity of this sensor was obtained as 27.43 mV/lg (N-protein concentration), which was higher than a regular H-diamond sensor. In addition, it had a wider linear detection range of 10−15–10−5 mg/ml and a lower limit of detection of 10−15 mg/ml. Accordingly, the detection performance of diamond sensor has been greatly improved due to the functionalization of Au nanoparticles.

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Trace detection of SARS-CoV-2 N-protein by diamond solution-gate field-effect transistor

Cited by 4 publications

References 25 publications

AFM-IN-SEM analyses of thiorphan assembly on ZnO polar and nonpolar surfaces

AFM-IN-SEM analyses of thiorphan assembly on ZnO polar and nonpolar surfaces

A biosensor based on magnetoelastic waves for detection of antibodies in human plasma for COVID-19 serodiagnosis

Optimization of diamond sensor for trace detection of SARS-CoV-2 N-protein using Au nanoparticles

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