Trace Determination of Tamoxifen in Biological Fluids Using Hollow Fiber Liquid-Phase Microextraction Followed by High-Performance Liquid Chromatography-Ultraviolet Detection

Abstract

The applicability of hollow fiber liquid-phase microextraction (HF-LPME) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was evaluated for the extraction and determination of tamoxifen (TAM) in biological fluids including human urine and plasma. The drug was extracted from a 15 mL aqueous sample (source phase; SP) into an organic phase impregnated in the pores of the hollow fiber (membrane phase; MP) followed by the back-extraction into a second aqueous solution (receiving phase; RP) located in the lumen of the hollow fiber. The effects of several factors such as the nature of organic solvent, compositions of SP and RP solutions, extraction time, ionic strength and stirring rate on the extraction efficiency were examined and optimized. An enrichment factor of 360 along with substantial sample clean up was obtained under the optimized conditions. The calibration curve showed linearity in the range of 1 -500 ng·mL -1 and the limit of detection was found to be 0.5 ng·mL -1 in aqueous medium. A reasonable relative recovery (≥89%) and satisfactory intra-assay (3.7% -4.2%, n = 3) and inter-assay (7.5% -7.8%, n = 3) precision illustrated good performance of the analytical procedure in spiked human urine and plasma samples.

Electrospun polyethylene terephthalate/graphene oxide nanofibrous membrane followed by HPLC for the separation and determination of tamoxifen in human blood plasma

“…Analytical parameters of introduced method were compared with some of previous TAM determination techniques and the results were listed in Table S2 . The comparative results show that the dynamic linear range of presented method is wider than the other introduced methods, which can be due to the presence of porous and GO nanoparticles in adsorbent structure and high absorption capacity of electrospun PET/GO nanofibers.…”

Analysis of tamoxifen and its main metabolites in plasma samples of breast cancer survivor female athletes: Multivariate and chemometric optimization

“…SPE not only needs a large quantity of the solvent and sample but also is timeconsuming and tedious [39][40][41]. Hollow fiber liquid-phase microextraction (HF-LPME) and single drop microextraction (SDME) were formerly applied for preconcentration of TAM in different matrices [42,43]. The extraction time for HF-LPME (60 min) is longer than SDME (25 min), while the time for the proposed technique is < 1 min.…”

PVC-Membrane Potentiometric Sensor for the Determination of Tamoxifen in Pharmaceutical Formulations

“…Tamoxifen therapy decreases the recursion risk of breast cancer and improves overall survival rates [4]. Tamoxifen's primary mechanism of action is competitive inhibition of the estrogen α-receptor by inhibiting growth of malignant breast cells [5]. However, tamoxifen doesn't prove same benefits for all women, and the side effect profiles change considerably from patient to patient [6].…”