Trace determination of trimetazidine in plasma by high-performance liquid chromatography using fluorescence detection

Sensitive and rapid LC–ESI-MS method for the determination of trimetazidine in human plasma

“…LC-ESI-MS analyses were performed using an Agilent Technologies Series 1100 LC/MSD VL system (Agilent Technologies, Palo Alto, CA) with a phenomenex Luna 5 C 18 (2) 100A HPLC column (250 mm × 4.60 mm, 5 m, Torrance, CA, USA). The mobile phase was 10 mM ammonium acetate buffer solution containing 0.1% acetic acid-methanol (45:55, v/v) at a flow rate of 1 ml/min.…”

“…It is only efficacious against the ischaemiainduced loss of membrane functions and its consequences [1]. Several HPLC [2,3], GC-MS [4] and LC-APCI-MS/MS [5] methods have been reported for the determination of trimetazidine in plasma, in which the most sensitive assay is the LC-APCI-MS/MS with an LLOQ of 1.5 ng/ml. The pilot pharmacokinetic study results in our laboratory showed that some of the human plasma levels of trimetazidine on the terminal elimination phase were below 1.5 ng/ml.…”

Non-extractive procedure followed by LC/APCI MS/MS analysis of trimetazidine in plasma samples for assessing bioequivalence of immediate/modified release formulations

“…HPTLC (Thoppil et al, 2001a) and reversed-phase (RP)HPLC (Thoppil et al, 2001b) stability-indicating methods for TMZ as a bulk active substance or in pharmaceutical formulations have been published. The first attempt to quantitatively assay TMZ in plasma samples was made using HPLC coupled to fluorescence detection (Courte and Bromet, 1981). TMZ was detected as a fluorescent dansyl derivative.…”

Sensitive determination of trimetazidine in spiked human plasma by HPLC with fluorescence detection after pre-column derivatization with 9-fluorenylmethyl chloroformate

“…Derivatives of 9-fluorenylmethyl chloroformate (FMOC-Cl) have been widely used for HPLC determination of a large variety of amines [6] but not applied for TMZ or FLX. Courte and Bromet described an HPLC method for the determination of TMZ with fluorescence detection [7]. The method was based on derivatization of TMZ with dansyl chloride.…”

“…Other reported methods have been adopted for the analysis of TMZ in bulk or pharmaceutical dosage forms which include voltammetry and HPLC with UV detection [12][13][14][15]. With the exception of the HPLC-fluorescence procedure [7], all other analytical methods do not confer enough sensitivity for monitoring low TMZ levels in plasma. The British Pharmacopoeia cited an HPLC method with UV detection at 240 nm for purity testing of TMZ in bulk form [16].…”