Trace element and lipidomic analysis of bottlenose dolphin blubber from the Yucatan coast: Lipid composition relationships

Ruiz-Hernández, Ixchel M.; Nouri, Mohammad‐Zaman; Kozuch, Marianne; Denslow, Nancy D.; Díaz-Gamboa, Raúl E.; Rodríguez-Canul, Rossanna; Collí-Dulá, Reyna Cristina

doi:10.1016/j.chemosphere.2022.134353

Cited by 12 publications

(4 citation statements)

References 78 publications

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“…The result of the present study found that the total RNA extracted from biopsy samples was of adequate quality and quantity for gene expression analysis. The fact that the blubber RIN value remained at an average of 6.4, despite immediate on‐site freezing of samples in liquid nitrogen after collection and the use of an adipose‐specific RNA extraction kit, could be attributed to RNase inhibitor penetration interference by abundant triglycerides in the blubber (Ruiz‐Hernández et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Blubber biopsy in common bottlenose dolphins using a novel biopsy puncher: Evaluation of the impact on living individuals and possibility of applications in cetacean research

Funasaka,

Suzuki,

Hosono

et al. 2024

J Exp Zool Pt A

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Biopsy has recently become a preferred protocol for sampling the skin and blubber of many cetacean species, although it is desirable to collect as minimally invasive as possible. Here, the effect of biopsy sampling on the captive common bottlenose dolphins was evaluated by analyzing the process of wound healing and changes in hematological and blood biochemical parameters after biopsy using a puncher developed to collect up to the inner layer of the dolphin blubber. Results showed that the wounds caused by biopsy were closed in as early as 1 day and completely covered with the epidermis within 5–11 days. Blood fibrinogen, which generally increases due to a wound‐induced inflammatory response or activation of the coagulation system, was significantly elevated after the biopsy indicating ongoing tissue repair, while other parameters did not exhibit significant differences. Furthermore, histological observation and RNA extraction of samples were performed to investigate the versatility of this method to cetacean research. Histological examination revealed three distinct layers of the blubber in the biopsy samples. Moreover, total RNA extracted from biopsy samples exhibited sufficient quality and quantity for gene expression analyses. Overall, the puncher utilized in our study represents a valuable and minimally invasive tool for investigating various aspects of small cetacean studies.

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Section: Discussionmentioning

confidence: 99%

Blubber biopsy in common bottlenose dolphins using a novel biopsy puncher: Evaluation of the impact on living individuals and possibility of applications in cetacean research

Funasaka,

Suzuki,

Hosono

et al. 2024

J Exp Zool Pt A

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“…Bottlenose dolphins are top predators, living in coastal environments and feeding on commercial species or interacting with fisheries and many other human related activities. For these reasons and because they are also included in Annex II of the Habitat Directive (EU Directive 92/43/CEE) they could be considered good candidates as a sentinel species to monitor both environmental and human health, taking advantage of the bioaccumulation phenomena [29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Cetacean Cell-Derived Extracellular Vesicles

Moccia,

Centelleghe,

Giusti

et al. 2023

Animals

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Cetaceans are of scientific interest because they are good candidates as environmental bioindicators. However, in vivo research is arduous and in vitro studies represent a rarely used valid alternative. Extracellular vesicles (EVs) are membrane-bound structures playing roles in cell-to-cell communication. Despite being a promising investigative tool in different fields of science, EVs have been poorly studied in cetaceans. To fill this gap, we describe the preliminary characterization of EVs isolated from a bottlenose dolphin and a Cuvier’s beaked whale cell line. EVs have been isolated with ultracentrifugation (UC) or size exclusion chromatography (SEC) and characterized with nanoparticle tracking analysis (NTA), Western blotting (WB), and scanning transmission electron microscopy (STEM). UC and SEC allowed the isolation of mainly small EVs (<200 nm). A higher number of particles were isolated through UC compared to SEC from both cell lines. At WB, all EVs expressed the EV-markers CD9 and integrin-β. Only EVs isolated with UC were positive for TSG101. In conclusion, we isolated for the first time EVs from a bottlenose dolphin and a Cuvier’s beaked whale cell line using two different techniques. Further studies on cell-derived EVs will be useful to deepen our knowledge on cetacean pathophysiology and health status assessment.

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“…The samples were collected in situ from the lateral area, on the flank, below the dorsal fin of dolphins; except for dolphins Tt3, Tt6, Tt10 and Tt12, for which samples were collected from the lowest region of the dorsal fin. All the analysis was performed as is mentioned in [1] . Briefly, statistical processing was done using R software [2] .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Trace elements and lipidomic datasets of stranding samples in the blubber of Turpsios truncatus from the Yucatan coast: Association with biological features

et al. 2023

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Trace element and lipidomic analysis of bottlenose dolphin blubber from the Yucatan coast: Lipid composition relationships

Cited by 12 publications

References 78 publications

Blubber biopsy in common bottlenose dolphins using a novel biopsy puncher: Evaluation of the impact on living individuals and possibility of applications in cetacean research

Blubber biopsy in common bottlenose dolphins using a novel biopsy puncher: Evaluation of the impact on living individuals and possibility of applications in cetacean research

Isolation and Characterization of Cetacean Cell-Derived Extracellular Vesicles

Trace elements and lipidomic datasets of stranding samples in the blubber of Turpsios truncatus from the Yucatan coast: Association with biological features

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