Trace element distribution and species fractionation in Brassica napus plant

Trace elements species fractionation in rye flour and rye (Secale cereale L.) seedlings

“…The element species fractionation in an extract of rye flour showed quite a similar pattern as did the analysis of the extracts of amaranth, buckwheat, and soybean flours, legume seeds, and rapeseeds Mestek et al 2002Mestek et al , 2007a. Particularly, dominant low-molecular weight fractions of Cu and Zn (1-2 kDa) and Mo (3 kDa) were typical for most of these samples.…”

“…Then 1 ml of the antioxidant solution (0.2% DTT) was added and after 20 min of incubation at 20°C, the mixture was freeze-dried. The details of the procedure were described in the previous article (Mestek et al 2007a).…”

“…Our previous experiments proved that soluble low molecular binding compounds of Cu, Zn, and other metals in buckwheat, amaranth (Mestek et al 2007a), and rape (Mestek et al 2007b) are rich in dicarboxylic amino acids and do not contain PCs. In the present work an analogous approach to the element species fractionation based on size exclusion chromatography -inductively coupled plasma mass spectrometry was applied for the analysis of rye flour and tissues of rye plants cultivated hydroponically both in normal fertiliser solution and in cadmium-enriched one.…”

Analyses of trace metals, peptide ligands of trace metals and mercury speciation in home prepared bread

“…plant metallothioneins called phytochelatins (PC), which play an important role in metal binding (Cobbet, 1999;Rauser, 1999;Zenk, 1996), especially in plants exposed to high metal ions concentrations. In our previous papers (Mestek et al, 2007(Mestek et al, , 2007a(Mestek et al, , 2010Polak et al, 2009), we pointed out that peptides rich in aspartic and glutamic acids can also take part in metal binding in plants. However, it should be noted that peptides are not the only metal-binding compounds.…”

Resposta da couve-da-Malásia (Brassica chinensis L. var. parachinensis (Bailey) Sinskaja) à deficiência nutricional

“…Em função dessa capacidade extratora as brássicas estão sendo testadas com sucesso para detectar metais pesados em substratos diversos, sendo candidatas à fitorremediação (HAN et al, 2004;PODAR et al, 2004;LINDBLOM et al, 2006;LI-XIAN et al, 2007;QI-TANG et al, 2007). Brassica napus L. acumula grandes quantidades de Fe, Mn, Zn, As, Cr, Ni, Cu, Pb, Co, Mo, Cd, Ag e Tl na raiz, mas a transferência desses metais para a parte aérea das plantas é limitada e para as sementes mais ainda, o que garante sua qualidade como fonte de alimento humano (MESTEK et al, 2007). Brassica oleracea L. var.…”