In our study, some in vitro biological activities of hexane (HEG), ethylacetate (EtOAc) and methanol (MeOH) extracts of Acanthus dioscoridis L. var. dioscoridis L. aerial parts have been investigated in details. Plant extracts were tested in view of its in vitro antioxidant activities as total phenolic and total flavonoid contents, DPPH free radical-scavenging, metal chelating and reducing power activity. Total phenolic content of MeOH extract has been found as 71.18 μg GAE/mg. The highest amount of flavonoids has been detected in EtOAc extract. The highest DPPH radical-scavenging activity and reducing power activity has been determined in MeOH extract. HEG extracts of Acanthus dioscoridis has showed clearly higher metal chelating activity when compared to other extracts. SPUFA percentage was meaningfully higher than SMUFA and SSFA. By considering the high PUFA content and strong antioxidant activities it could be concluded that A. dioscoridis could be recommended to possible use in medical science, cosmetic and food industry.