The present study explores the effect of salinity and dissolved organic carbon (DOC) gradients on the stability and reactivity of titanium dioxide nanoparticle (TiO 2 -NP) agglomerates in ambient water from the Lagoon of Venice and their possible effect on nauplii sampled at the same locations. In all ambient water samples, TiO 2 -NPs formed rapidly micrometre-sized agglomerates. The increase in the salinity and concomitant decrease in DOC content induced the formation of larger agglomerates, with z-average hydrodynamic diameter increasing with TiO 2 -NP concentration and exposure duration. Under the studied conditions, f-potential exhibited negative values. In line with agglomeration results, enhancement of the salinity and lower DOC resulted in less negative fpotential with close to 0 values in the dispersions of 100 mg L -1 TiO 2 -NPs in sea water. Two-hour exposure to micrometre-sized agglomerates of TiO 2 -NPs resulted in an increase in the fluorescence of propidium iodide (PI) stained nauplii in comparison with unexposed controls, but had no effect at 24-h exposure. The increase in nauplii-associated PI fluorescence was more noticeable in dispersions containing 100 mg L -1 than those containing 10 mg L -1 TiO 2 -NPs, suggesting membrane permeability alteration in a concentrationdependent manner. However, the PI staining results have to be interpreted with caution because of the possible dye binding to the nauplii surface without penetration of cellular membrane. The effect of TiO 2 -NPs on nauplii was more pronounced at higher salinity and decreased with increasing DOC concentrations at 2 h, while no trends were found at 24-h exposure, as well as exposure to 100 mg L -1 TiO 2 -NPs.