Trace Metals in the Freshwater Fish Cyprinus carpio: Effect to Serum Biochemistry and Oxidative Status Markers

Kováčik, Anton; Tvrdá, Eva; Miškeje, Michal; Árvay, Július; Tomka, Marián; Zbyňovská, Katarína; Andreji, Jaroslav; Hleba, Lukáš; Fik, Martin; Nahácky, Jozef; Massányi, Peter

doi:10.1007/s12011-018-1415-x

Cited by 38 publications

(22 citation statements)

References 67 publications

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“…Uric acid was found to decrease under arsenic stress on C. gariepinus of the present work and that of Ogamba et al (2011). Different other studies revealed an increase in uric acid under arsenic stress (Kumari, 2015) and different other pollutants (Mutlu et al, 2015;Kovacik et al, 2019).…”

Section: Discussionmentioning

confidence: 84%

Significance Assessment of Amphora coffeaeformis in Arsenic-Induced Hemato- Biochemical Alterations of African Catfish (Clarias gariepinus)

et al. 2020

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Heavy metals have different adverse impacts on different life stages of fish species with attempts to use natural antioxidants to counteract their effects. So, the present study investigated the potential protective effects of Amphora coffeaeformis extract against arsenic-induced hemato-biochemical alterations in African catfish, Clarias gariepinus. The fish exposed to sub-lethal concentrations of arsenic; 19.2 and 38.3 mg/L (1/8 and 1/4 of 96h-LC 50 value, 153.17 mg/L) for 15 days. The main effect of arsenic was recorded in some blood parameters such as RBC's count, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, and white blood cells. As for biochemical parameters, the main effect of arsenic was significant for alkaline phosphatase, glucose, uric acid, creatinine, albumin, globulin, and albumin/globulin. Also, the residue of arsenic in fish muscles showed significant effects. The majority of these arsenic-induced parameters were improved with dietary supplements of the diatom A. coffeaeformis. So, Amphora extract can be used as detoxification factor on fishes induced by arsenic due to its biologically active components providing protections like antioxidant, antiviral, antibacterial, and anti-inflammatory. Besides, they have excellent contents of proteins and carbohydrates which are supposed to enhance the effect of these compounds.

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Section: Discussionmentioning

confidence: 84%

Significance Assessment of Amphora coffeaeformis in Arsenic-Induced Hemato- Biochemical Alterations of African Catfish (Clarias gariepinus)

et al. 2020

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“…Biochemical blood factors are diagnostic tools for evaluating the metabolic rate (blood protein and globulin), kidney function (creatinine, bilirubin, and uric acid), liver function (albumin, ALT, AST, and ALP), and stress indices (cortisol and glucose) of the organism [ 49 , 50 ]. Under toxicity conditions, the levels of these indices may deteriorate, referring to dysfunction of vital organs involved in renal, metabolic, antioxidative, and immunity roles in the fish body [ 6 ].…”

Section: Discussionmentioning

confidence: 99%

Role of Fucoidan on the Growth Behavior and Blood Metabolites and Toxic Effects of Atrazine in Nile Tilapia Oreochromis niloticus (Linnaeus, 1758)

Abdel-Warith

Younis

Al-Asgah

et al. 2021

Animals

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Waterborne herbicides are stressful agents that threaten the productivity and safety of finfish species. In this study, the toxicity impacts of atrazine (ATZ) and the protective role of fucoidan were investigated on the health performance of Nile tilapia. For 40 days, the total number of 180 Nile tilapia was assigned in four groups (triplicates, 15 fish/replicate), where the first (control) and third groups were offered the control diet, while the second and fourth groups were offered a fucoidan (FCN). Further, in the third and fourth groups, the water was mixed with atrazine (ATZ) at 1.39 mg/L daily. The growth rate, FCR, and survival rate were markedly enhanced by fucoidan but severely declined by ATZ exposure (p < 0.05). The morphological structure of the intestine in the control fish revealed normal structure, while fucoidan-treated groups showed eminent enhancement and branching of the intestinal villi. The intestine of ATZ-treated fish revealed deterioration and the intestinal mucosa, inflammatory cell infiltration, and separation of lining epithelium. The highest Hb, PCV, RBCs, WBCs, total protein, and albumin were observed in Nile tilapia fed fucoidan, but the worst values were seen in ATZ-intoxicated fish (p < 0.05). The liver-related enzymes (ALT and AST) and kidney function (urea and creatinine) showed impaired values by ATZ toxicity and were regulated by dietary fucoidan. Meanwhile, fish fed fucoidan and exposed to ATZ had lower total cholesterol and triglyceride values than fish exposed to ATZ without fucoidan feeding (p < 0.05). The SOD, CAT, GPx, cortisol, and glucose levels were increased in ATZ-exposed fish and reduced by fucoidan (p < 0.05). However, the level of malondialdehyde (MDA) was reduced in fucoidan-fed fish and increased in ATZ-exposed fish (p < 0.05). Altogether, dietary fucoidan is required in fish diets to alleviate the impacts of ATZ-induced toxicity.

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“…For analysis of cortisol DIALAB Elisa test kit (DIALAB Pruduktion and Vertrieb von chemish – technischen Produkten und Laborinstrumenten Gesellschaft m. b. H., Wiener Neudorf, Austria) was used. Mineral profile analysis was measured using commercial DiaSys (Diagnostic Systems GmbH, Holzheim, Germany) on the Randox RX Monza analyser (Crumlin, United Kingdom) ( Kováčik et al, 2017 , Kovacik et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

The effect of different sample collection methods on rabbit blood parameters

Massányi

Kohút

Argente

et al. 2020

Saudi Journal of Biological Sciences

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Nowadays great deal of research is physiological field is conducted on experimental animals and there is a lot of criticism from the wide public on methods used. Therefore, recently there is a lot of effort focused on the welfare of the animals. Main aim of this study is to determine the effect of experimental sample collection method on the selected parameters of stress. In the experiment two sample collections of rabbit blood from marginal ear vein were realized – first using standard method with one person fixing the animal and other collecting the blood using gently fixating the animal. In the second groups experimental method of inserting the experimental animal into a sack and further collection in dark was realized. During the experiment the levels of cortisol – main stress indicator in organism and other health parameters of animals including mineral profile and haematological parameters were observed. Our results show no significant changes in levels of cortisol but also a decreasing tendency in the sample from the second (dark) collection. Haematological parameters were generally in the reference values and any significant changes except levels of lymphocytes and percent of lymphocytes which shown significant increase in the second collection period were found. Also the levels of mean corpuscular haemoglobin and percent of neutrophils unveiled a significant decrease in values. Values of mineral profile parameters have indicated no significant changes except the levels of phosphorus. Based on the result we can state that the experimental sample collection has no effect on blood parameters of the animals but we spectated a statistically insignificant decrease in the levels of cortisol which can suggest that the dark collection is possibly less stressful to the animals.

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Trace Metals in the Freshwater Fish Cyprinus carpio: Effect to Serum Biochemistry and Oxidative Status Markers

Cited by 38 publications

References 67 publications

Significance Assessment of Amphora coffeaeformis in Arsenic-Induced Hemato- Biochemical Alterations of African Catfish (Clarias gariepinus)

Significance Assessment of Amphora coffeaeformis in Arsenic-Induced Hemato- Biochemical Alterations of African Catfish (Clarias gariepinus)

Role of Fucoidan on the Growth Behavior and Blood Metabolites and Toxic Effects of Atrazine in Nile Tilapia Oreochromis niloticus (Linnaeus, 1758)

The effect of different sample collection methods on rabbit blood parameters

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