Traceability of different brands of bottled mineral water during shelf life, using PCR-DGGE and next generation sequencing techniques

Sala-Comorera, Laura; Blanch, Anicet R.; Casanovas‐Massana, Arnau; Monleón-Getino, Toni; García-Aljaro, Cristina

doi:10.1016/j.fm.2019.01.006

Cited by 17 publications

(18 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result agreed with the results of Bedada et al (2018) and Shahryari et al (2020) who in their respective studies found that 51.4% and 61.8% samples were found to exceed the guideline value. In this study, the HPC count was in the range of 15-1,300 cfu/ml which agreed with the result of Bedada et al (2018), Sala-Comorera et al (2019) and Shahryari et al (2020) in which the range of HPC was found to be 1-99, 1-10,000 and 9,974-23,855 cfu/ml. HPC are a good indicator of the overall quality of production (WHO, 2017) and as recent studies have revealed that trace elements are present in ample amount in bottled water, the extremophiles may survive due to the optimum osmotic pressure (maintained by the microorganism in the bottled water) (Gautam, 2020;Gautam & Adhikari, 2018a;Gautam et al, 2021).…”

Section: Discussionsupporting

confidence: 90%

“…(2018), Sala‐Comorera et al. (2019) and Shahryari et al (2020) in which the range of HPC was found to be 1–99, 1–10,000 and 9,974–23,855 cfu/ml. HPC are a good indicator of the overall quality of production (WHO, 2017) and as recent studies have revealed that trace elements are present in ample amount in bottled water, the extremophiles may survive due to the optimum osmotic pressure (maintained by the microorganism in the bottled water) (Gautam, 2020; Gautam & Adhikari, 2018a; Gautam et al., 2021).…”

Section: Discussionmentioning

confidence: 91%

See 1 more Smart Citation

Microbiological quality assessment (including antibiogram and threat assessment) of bottled water

Gautam

2021

Food Science & Nutrition

View full text Add to dashboard Cite

Water pollution is a major global problem that has been the leading cause of morbidity and mortality. This study was carried out to assess the microbiological quality of popular domestic brands of bottled water available in the Kathmandu Valley in Nepal. For the study of bottled water, a total of 50 samples each of different volumes (20 and 1 L) were selected. The samples were processed at the Microbiology laboratory at St. Xavier's College, Maitighar, Kathmandu. The microbiological assessments were performed as per the methods described in the American Public Health Association, 2005. Out of 100 samples, 48% of samples were found to be contaminated with total coliform. Escherichia coli was the predominant strain among the coliforms. Multidrug‐resistant E. coli, Enterobacter aerogenes, and Pseudomonas aeruginosa were isolated from the 48 bottled water. Treat assessment test revealed that 88.23% of the isolated E. coli produced β hemolytic colonies, while 11.77% did not show hemolytic colonies, 100% E. aerogenes colonies were not able to develop hemolytic colonies and 100%, P. aeruginosa colonies gave β hemolytic colonies, respectively. Chi–square test shows that there is a significant association (p ≤ .05) between fecal coliform and volume of the vessel (i.e., 20 and 1 L), bottle type (i.e., unscratched and undented and scratched and/or dented), season (i.e., monsoon and postmonsoon), and total coliform. Chi‐square test shows that there is no significant (p > .05) association between Pseudomonas spp and season (i.e., monsoon and postmonsoon). Out of 100 samples, 48% of samples were found to be contaminated with total coliform. Microorganisms survive in bottled water as they have many nutrients required for the microorganism in ionic form. Surveillance is lacking by the license‐providing organizations followed by governmental organizations.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 91%

Microbiological quality assessment (including antibiogram and threat assessment) of bottled water

Gautam

2021

Food Science & Nutrition

View full text Add to dashboard Cite

show abstract

“…For example, in the research conducted by Sala-Comorera et al [9] in 2019, the genus Polaromonas resulted ubiquitous in being detected in more than 50% of bottled mineral water samples: genus Polaromonas was detected in the present study in sample B (54%) and C (3%). Strains from Acidovorax (samples C and E), Bacillus (in A, D and F), Novosphingobium (in B, C, D, E and F), Pseudomonas (in A, B, D and E), Rhizobium (in A, C and F), Staphylococcus (in A, B, E and F) and other genera were detected in similar studies [7][8][9]23,48]. Furthermore, strains of Strenotrophomonas and Sulfuritalea genera, isolated employing cultural methods but not with the NGS approach, were also typical of mineral waters analyzed in current studies [48].…”

Section: Comparison With Similar Studiessupporting

confidence: 65%

“…The indicated values are measured within 12 h after the bottling process. Despite the fact that European and International regulations establish the exclusive research of the culturable bacteria in mineral bottled waters [4,5], these matrices contain further microorganisms, which are not easily detectable employing the standardized cultural methodologies [6][7][8], thus underestimating the real quantity and quality of bacteria contained in the drinking bottled water, additionally representing a risk for consumers. Recent studies suggest the employment of culture-independent methods to characterize the dynamics of the microbial community over time inside the bottle [7,9]: the studies report bacterial reactivation along with bottled water storage, together with the succession of microbial communities along with shelf life, probably because of the exploitation of dead microorganisms' organic matter [9,10].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Microbial Communities of Bottled Mineral Waters and Preliminary Traceability Analysis Using NGS Microbial Fingerprints

Carraturo

Giudice

Compagnone

et al. 2021

Water

View full text Add to dashboard Cite

The microbiological monitoring of mineral bottled waters results is crucial for the prevention of outbreaks in consumers. European and International regulations establish the quality of water intended for human consumption in order to preserve human health from the negative effects deriving from water contamination. Advanced methods targeting the faster detection of potential pathogens in drinking water may consent to the creation of an early warning system, enhancing water quality management. This study aimed to suggest the implementation of standard water quality evaluations, based on the characterization of the microbial composition of mineral bottled water brands, contributing to the periodic control of the water’s microbiological stability along with the shelf life, and, consequently, the stability of the supplying sources. Bottled water microbiota analysis was combined with the qualitative and quantitative evaluation of microbial loads in time, and the monitoring was performed in two seasons and two different storage conditions for a total of sixty days. The employment of molecular microbiology techniques (NGS and Sanger sequencing), compared to standardized cultural methods and integrated with metagenomic analysis, combining chemical and physical indicators for each sample, allowing for the generation of specific fingerprints for mineral bottled waters, pointing at simplifying and improving the foreseen risk assessment strategies to ensure the adequate traceability, quality and safety management of drinking water.

show abstract

“…We directed this research to examine the distribution of bacterial communities and the impact of water temperature. To determine the bacterial distribution through the region, Illumina next-generation sequencing analyses [23][24][25] have been used to identify bacteria from water samples in the study region.…”

Section: Introductionmentioning

confidence: 99%

Spatial Modelling of Bacterial Diversity over the Selected Regions in Bangladesh by Next-Generation Sequencing: Role of Water Temperature

et al. 2020

View full text Add to dashboard Cite

In this study, a spatial model has been developed to investigate the role of water temperature to the distribution of bacteria over the selected regions in the Bay of Bengal, located in the southern region of Bangladesh using next-generation sequencing. Bacterial concentration, quantitative polymerase chain reactions, and sequencing were performed on water samples and identified Acidobacteria, Actinobacteria, Bacteroidetes, Chlorobi, Chloroflexi, Cyanobacteria, Firmicutes, Nitrospirae, Planctomycetes, Proteobacteria, and Verrucomicrobia. The spatial model tessellated the parts of the Bay of Bengal with hexagons and analyzed the relationship between the distribution of bacteria and water temperature. A geographically weighted regression was used to observe whether water temperature contributed strongly or weakly to the distribution of bacteria. The residuals were examined to assess the model's fitness. The spatial model has the potential to predict the bacterial diversity in the selected regions of Bangladesh.

show abstract

Traceability of different brands of bottled mineral water during shelf life, using PCR-DGGE and next generation sequencing techniques

Cited by 17 publications

References 36 publications

Microbiological quality assessment (including antibiogram and threat assessment) of bottled water

Microbiological quality assessment (including antibiogram and threat assessment) of bottled water

Evaluation of Microbial Communities of Bottled Mineral Waters and Preliminary Traceability Analysis Using NGS Microbial Fingerprints

Spatial Modelling of Bacterial Diversity over the Selected Regions in Bangladesh by Next-Generation Sequencing: Role of Water Temperature

Contact Info

Product

Resources

About