Traceback of the Psoroptes outbreak in British Columbian bighorn sheep (Ovis Canadensis)

Abstract

Psoroptes are a non-burrowing, ectoparasitic, mange-causing mite that has been documented in American bighorn sheep populations throughout the 19th and 20th centuries; however, it was not seen on Canadian bighorn sheep until 2006. The aim of this study was to determine the potential source of the Psoroptes outbreak in Canadian bighorn sheep. Morphological and molecular analyses were used to compare mites recovered from outbreak-associated bighorn sheep, pet rabbits in Canada, and on historically infested bighorn sheep in the USA. The results revealed that Psoroptes acquired from the Canadian and outbreak-associated American bighorn sheep were morphologically more similar to those collected from rabbits than mites on historically infested bighorn sheep. Outer opisthosomal setae lengths measured an average of 81.7 μm (±7.7 μm) in outbreak associated bighorn mites, 88.9 μm (±12.0 μm) in rabbit mites and 151.2 μm (±16.6 μm) in historically infested bighorn mites. The opisthosomal lobe morphology of bighorn mites in the outbreak herds was also more similar to that of rabbit mites, previously described as P. cuniculi , than historically infested bighorn mites, which match previous descriptions of P. ovis . This finding was supported by DNA sequence data of the mitochondrial cytochrome B gene. This is the first report of Psoroptes of the rabbit ecotype on bighorn sheep. The morphological and molecular data therefore support the hypothesis that the source of Psoroptes outbreak in Canadian bighorn sheep represented a disease spillover event from rabbits rather than transmission from infested American bighorn sheep populations.

Morphologic and Molecular Characterization of Psoroptes ovis from Pet Rabbits in South Korea

“…Genetic analysis using the cox1 gene confirmed the mite species as P. ovis. Previous research has highlighted the reliability of the cox1 gene as a molecular marker for species identification and phylogenetic analysis in mites (11,23). The high sequence identity (99%) with the previously reported sequence (MW590279) of P. ovis from the NCBI database emphasizes the accuracy of this molecular approach.…”

“…The cytochrome c oxidase subunit 1 (cox1) gene has been extensively utilized as a molecular marker for species identification and phylogenetic analysis of mites including P. ovis (11,23). The primary objective of this work is to analyze mites obtained from pet rabbits in South Korea through cox1 gene sequencing.…”

Sheep scab spatial distribution: the roles of transmission pathways

“…These transmission routes were not explored here since they are not considered to be epidemiologically significant for Psoroptic mange [ 42 ] , even though P. ovis is not considered to be host specific [ 43 ]. Transmission of P. ovis between host species is thought to be a rare occurrence in the field and requires enforced close contact, as seen in a potential P. ovis transmission event between rabbits and bighorn sheep in North America held in captivity together at a game farm [ 44 ].…”