Tracheal matrices, obtained by a detergent-enzymatic method, support in vitro the adhesion of chondrocytes and tracheal epithelial cells

Abstract

Several attemps have been performed to achieve a suitable tracheal replacement for the treatment of different conditions characterized by a lack of sufficient tissue for surgical reconstruction. Actually, tracheal homografts can induce long-term stenosis and their growth potential is not known. Thus, in this work porcine tracheal matrices have been obtained by a detergent-enzymatic method. The treatment decreased the antigenicity of matrices which were able to support the in vitro adhesion of both chondrocytes and tracheal epithelial cells. On the contrary, only few cells were observed in tracheal matrices prepared with formalin, Thimerosal, and acetone, suggesting that the long-term stenosis occuering in vivo is probably because of an insufficient cell ingrowth. In summary, our results indicate that the detergent-enzymatic method allows us to obtain tracheal matrices which can function as a promising support to achieve an in vitro tissue-engineered cell-matrix construc

Whole-organ engineering with natural extracellular materials

“…In addition, the effectiveness of cell removal and tissue damage varies based upon the method of decellularization (chemical, enzymatic or physical) [13,14,24,28]. Decellularization methods attempting to optimize decellularization reagent incubation time, temperature, concentration or number of solvent cycles, have been reported with varying degrees of success based upon histology, scanning-electron microscopy and mechanical testing of the resulting decellularized scaffold product [12,19,20,28].…”

“…In human allograft studies of tracheal tissue, optimized tissue penetration was achieved by circulating cycles of detergents to remove nucleic materials [19,20]. While verification of cell removal is typically quantified by DNA content post-decellularization by means of spectrophotometry [29][30][31][32], further characterization of the remaining major histocompatibility complexes (MHCs) is essential for clinical human allograft studies.…”

“…These scaffolds retain the original architecture of organs and tissue interfaces, giving rise to a source of allogenic and xenogenic whole--organ grafts. Decellularization has been carried out on a variety of tissues of various organs such as the urinary bladder, small intestinal submucosa, blood vessels, heart valves, pericardium, tooth buds, trachea and esophagus, as well as musculoskeletal regions such as the temporomandibular joint [9][10][11][12][13][14][15][16][17][18][19][20][21][22]. This method of creating acellular scaffolds has resulted in commercial products and tissue substitutes [23][24][25][26][27].…”

Desnutrição materna durante a lactação em ratos Wistar: efeitos sobre as fibras elásticas da matriz extracelular na traqueia dos filhotes

“…In hospitalized patients, the reported prevalence of malnutrition is as high as 75%. (1,2) In addition, hospitalization further worsens the nutritional status. Malnutrition has been associated with increases in the risk of in-hospital morbidity/ mortality, the length of hospital stay, the costs, and the use of health care resources.…”

“…In addition, the effects of protein malnutrition on tracheal histology are worth studying, given the prevalence of this condition in hospitalized patients, especially in those in developing countries. (1,3,6) An understanding of the effects of protein malnutrition on the elastic fibers in the tracheas of rats might improve the scientific knowledge base on the composition and organization of From each specimen, five tissue sections were analyzed, and from each section, five random fields were evaluated; therefore, we analyzed 25 test areas for each individual. The analyzed fields were digitized using a video camera coupled to a light microscope, with a final magnification of ×400.…”