Trachydiscus minutus, a new biotechnological source of eicosapentaenoic acid

Řezanka, Tomáš; Petránková, Mirka; Cepák,; Přibyl, Pavel; Sigler, Karel; Cajthaml, Tomáš

doi:10.1007/s12223-010-0039-0

Cited by 50 publications

(37 citation statements)

References 32 publications

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“…Meyer personal communication). Trachydiscus minu tus has been found to have high levels of the essential fatty acid eicosapentaenoic acid (Rezanka et al, 2010).…”

Section: Ecology and Distributionmentioning

confidence: 99%

Xanthophyte, Eustigmatophyte, and Raphidophyte Algae

Ott

Oldham-Ott

Rybalka

et al. 2015

Freshwater Algae of North America

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“…Meyer personal communication). Trachydiscus minu tus has been found to have high levels of the essential fatty acid eicosapentaenoic acid (Rezanka et al, 2010).…”

Section: Ecology and Distributionmentioning

confidence: 99%

Xanthophyte, Eustigmatophyte, and Raphidophyte Algae

Ott

Oldham-Ott

Rybalka

et al. 2015

Freshwater Algae of North America

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“…(Ben-Amotz and Avron 1983;Converti et al 2009). We have focused on such β-carotene-rich lipid bodies (LBs) of the microalga Trachydiscus minutus (Xanthophyceae), which additionally contains high proportions of PUFA in its storage lipids (Rezanka et al 2010;Samek et al 2010). Algae were cultivated in a light intensity gradient to induce a gradual increase of β-carotene and storage lipid production.…”

Section: Introductionmentioning

confidence: 99%

Raman microspectroscopy of algal lipid bodies: β-carotene quantification

et al. 2011

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Advanced optical instruments can serve for analysis and manipulation of individual living cells and their internal structures. We have used Raman microspectroscopic analysis for assessment of β-carotene concentration in algal lipid bodies (LBs) in vivo. Some algae contain β-carotene in high amounts in their LBs, including strains which are considered useful in biotechnology for lipid and pigment production. We have devised a simple method to measure the concentration of β-carotene in a mixture of algal storage lipids from the ratio of their Raman vibrations. This finding may allow fast acquisition of β-carotene concentration valuable, e.g., for Raman microspectroscopy assisted cell sorting for selection of the overproducing strains. Furthermore, we demonstrate that β-carotene concentration can be proportional to LB volume and light intensity during the cultivation. We combine optical manipulation and analysis on a microfluidic platform in order to achieve fast, effective, and non-invasive sorting based on the spectroscopic features of the individual living cells. The resultant apparatus could find its use in demanding biotechnological applications such as selection of rare natural mutants or artificially modified cells resulting from genetic manipulations.

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“…13,14 . We have focused on such β-carotene rich LBs of microalga Trachydiscus minutus (Xanthophyceae), which contains high proportions of PUFA in its storage lipids 1,15 . Algae were cultivated in a light intensity gradient to induce a gradual increase of storage lipid production.…”

Section: Introductionmentioning

confidence: 99%

Raman microspectroscopy of algal lipid bodies: β-carotene as a volume sensor

et al. 2011

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Advanced optical instruments are useful for analysis and manipulation of individual living cells and their internal structures. We have employed Raman microspectroscopic analysis for assessment of algal lipid body (LB) volume in vivo. Some algae contain β-carotene in high amounts in their LBs, including strains which are considered useful in biotechnology for lipid and pigment production. We have detected proportionality between the Raman vibrations of β-carotene and the LB volume. This finding may allow fast acquisition of LB volume approximation valuable e.g. for Raman microspectroscopy assisted cell sorting. We combine optical manipulation and analysis on a microfluidic platform in order to achieve fast, effective, and non-invasive sorting based on spectroscopic features of the individual living cells. The resultant apparatus could find its use in demanding biotechnological applications such as selection of rare natural mutants or artificially modified cells resulting from genetic manipulations.

show abstract

Trachydiscus minutus, a new biotechnological source of eicosapentaenoic acid

Cited by 50 publications

References 32 publications

Xanthophyte, Eustigmatophyte, and Raphidophyte Algae

Xanthophyte, Eustigmatophyte, and Raphidophyte Algae

Raman microspectroscopy of algal lipid bodies: β-carotene quantification

Raman microspectroscopy of algal lipid bodies: β-carotene as a volume sensor

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