Tracing anti‐osteoporosis components from raw and salt‐processed semen of Cuscuta chinensis by employing a biochemometrics strategy that integrates ultrasonic‐assisted extraction, quantitation, efficacy assessment in zebrafish, and grey relationship analysis

Abstract

Semen of Cuscuta chinensis has been reported to have an anti‐osteoporosis effect, however, the components which account for the anti‐osteoporosis effect have not been clarified. In this work we propose a biochemometrics strategy that integrates quantitation, anti‐osteoporosis evaluation in zebrafish, and grey relationship analysis for the identification of anti‐osteoporosis components from the semen of Cuscuta chinensis. In the beginning, a precise and accurate liquid chromatography‐tandem mass spectrometry method was established for simultaneous quantitation of seven major components in crude and salt‐processed Cuscuta chinensis. The mode of multiple reaction monitoring was used. Chloramphenicol was selected as the internal standard. The method showed good linearity and repeatability. The recovery rates of each component ranged from 95.4 to 103.9%. The precisions of intra‐day and inter‐day were all within 5.0%. The method was then applied for quantitation of the seven major components in 11 batches of crude and salt‐processed Cuscuta chinensis. Subsequently, the anti‐osteoporosis effects of crude and salt‐processed Cuscuta chinensis were evaluated in zebrafish. Principle component analysis, grey relationship analysis, and partial least squares regression were applied for deciphering the relationship between the contents of seven major components and the anti‐osteoporosis effects. Hyperin, p‐hydroxycinnamic acid, and astragalin were found to be the major anti‐osteoporosis components.

GC–MS and LC-TOF–MS profiles, toxicity, and macrophage-dependent in vitro anti-osteoporosis activity of Prunus africana (Hook f.) Kalkman Bark

“…In another study, Astragalin promoted differentiation in MC3T3-E1 osteoblastic cells through activation of MAPK and BMP pathways and promoted in vivo bone formation 28 . Further, a number of other studies have showed anti-osteoporosis activity of Astragalin 10 , 29 , 30 , and its presence in P. africana stem bark as confirmed in our study shows that it indeed contributes to the anti-osteoporosis activity of the plant stem bark. Hyperin was one of the major 3 chemical compounds in Cuscuta chinensis that showed anti-osteoporosis effects 29 .…”

“…Further, a number of other studies have showed anti-osteoporosis activity of Astragalin 10 , 29 , 30 , and its presence in P. africana stem bark as confirmed in our study shows that it indeed contributes to the anti-osteoporosis activity of the plant stem bark. Hyperin was one of the major 3 chemical compounds in Cuscuta chinensis that showed anti-osteoporosis effects 29 . In fact in their study, Tao, et al 29 ascribed the anti-osteoporosis effect of C. chinensis to hyperin as a result of its high positive correlation to anti-osteoporosis activity.…”

“…Hyperin was one of the major 3 chemical compounds in Cuscuta chinensis that showed anti-osteoporosis effects 29 . In fact in their study, Tao, et al 29 ascribed the anti-osteoporosis effect of C. chinensis to hyperin as a result of its high positive correlation to anti-osteoporosis activity. Hyperin was also reported to markedly increase alkaline phosphatase (ALP) activity in osteoblast cells 31 .…”

Anti‐osteoporosis bioactivity evaluation in zebrafish model of raw and salt‐processed Achyranthes bidentata followed by liquid chromatography–mass spectrometry analysis and correlation analysis

“…The evaluation of the anti‐osteoprotic activity of single compounds was conducted according to the literature (Tao et al, 2021). Zebrafish larvae with successful modeling were divided into 18 groups and given three concentrations of β ‐ecdysterone, wogonin, ginsenoside Ro, oleanolic acid, linoleic acid and palmitic acid solutions.…”

Anti-osteoporosis effects and regulatory mechanism of Lindera aggregata based on network pharmacology and experimental validation

“…13 Recent studies show that Semen cuscutae extract inhibits the apoptosis and promotes proliferation of osteoblasts. 9,14,15 Furthermore, it is indicated that Semen cuscutae exerted a satisfactory anti-osteoporosis therapeutic effect on OVX mice and its efficacious anti-osteoporosis activity is due to its thorough regulation of the c-fos/c-Src kinase/NFATC1 signaling pathway. 16 Bajitianwan (BJTW) is a classical traditional Chinese medicine formulation consisting of seven herbal drugs.…”