The rapid increase in population and economic activities has greatly influenced land use and spatial development. In urban agglomerations where socioeconomic activities are densely concentrated, the clash between ecological protection and economic growth is becoming more evident. Therefore, a thorough quantitative assessment of spatial changes driven by land use dynamics, alongside an examination of temporal and spatial driving factors, is crucial in offering scientific backing for the long-term and sustainable growth of urban agglomerations. This paper focuses on the major urban agglomerations in China’s Yangtze River Delta region, examining the spatiotemporal evolution of land use and landscape patterns from 2000 to 2020. By employing the standard deviation ellipse technique, coupled with multiple linear regression and the geographical detector model, we conduct a quantitative assessment of the directional trends in urban construction land expansion as well as the diverse impacts of temporal and spatial factors on this expansion across various periods and regions. The findings indicate that over the past 20 years, construction land in the Yangtze River Delta Urban Agglomeration expanded in concentrated patches, showing significant scale effects with relatively intact farmland and forest land being increasingly encroached upon. Landscape-type transitions predominantly occurred in cities around Taihu Lake and Hangzhou Bay, with the most significant transition being farmland converted to construction land, resulting in a greater number of patches and more pronounced land fragmentation. Throughout the 20 years, the standard deviation ellipse of construction land in the Yangtze River Delta Urban Agglomeration expanded and shifted, with the predominant expansion trending from the northwest toward the southeast, and the EN orientation being the most intense expansion area, covering 1641.24 km2. The influence of temporal and spatial driving factors on the expansion of urban construction land differed across various periods and regions. This study thoroughly examines the driving factors that affect the evolution of urban construction land in the region, offering valuable scientific evidence and references for future planning and development of the Yangtze River Delta Urban Agglomeration, aiding in the formulation of more precise and efficient urban management and land use strategies.