Tracing keratin evolution: Catalog, expression patterns and primary structure of shark (Scyliorhinus stellaris) keratins

Schaffeld, Michael; Löbbecke, Andrea; Lieb, Bernhard; Markl, Jürgen

doi:10.1016/s0171-9335(98)80074-5

Cited by 29 publications

(55 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…According to the expression sites, keratin genes can be divided into E (epidermal keratinocyte)-type and S (simple epithelia)-type (Conrad et al, 1998;Schaffeld et al, 1998). We found that the expression of ictacalcin is similar to E-type keratin, because the ictacalcin staining is detected solely in striated epithelial cells covering the entire embryo.…”

Section: Expression Of Ictacalcin In Envelope Cells and Keratinocytesmentioning

confidence: 83%

“…Our understanding on skin development in fish can be largely attributed to the expression of the keratin genes (Imboden et al, 1997;Schaffeld et al, 1998Schaffeld et al, , 2003Conrad et al, 1998;Martorana et al, 2001). According to the expression sites, keratin genes can be divided into E (epidermal keratinocyte)-type and S (simple epithelia)-type (Conrad et al, 1998;Schaffeld et al, 1998).…”

Section: Expression Of Ictacalcin In Envelope Cells and Keratinocytesmentioning

confidence: 99%

See 1 more Smart Citation

Skin‐specific expression of ictacalcin, a homolog of the S100 genes, during zebrafish embryogenesis

Hsiao

Ekker

Tsai

2003

Developmental Dynamics

View full text Add to dashboard Cite

Full-length cDNA coding for the ictacalcin gene, a homolog of the S100 genes, was isolated in zebrafish and mapped on linkage group 16 using the LN54 radiation hybrid panel. The homology and phylogenetic analyses, based on the deduced amino acid sequences, showed the orthologous relationship of ictacalcin genes between zebrafish and other fish species. However, ictacalcin genes constitute an out-group with respect to other members of the S100 gene family. This result supports the findings that fish ictacalcin genes are new members of the S100 gene family and may have evolved after the divergence of teleosts and tetrapods. The zebrafish ictacalcin gene was zygotically transcribed from 12 hours postfertilization onward and was stably expressed throughout adulthood. During zebrafish embryogenesis, the ictacalcin gene was specifically expressed in striated epidermal cells covering the entire embryo. The ictacalcin staining in keratinocytes of striated epithelia was absent in the cytoplasm surrounding the nuclei, but it was highly concentrated in the peripheral margin. Tissues enriched with epithelia folds, such as olfactory epithelium, hatching gland, pectoral fin buds, urogenital opening, and pharynx, showed a robust ictacalcin expression. The strikingly heavy staining of ictacalcin in the pharyngeal region provides us with an early marker to follow the pharynx formation in zebrafish embryos. Developmental Dynamics 228:745-750, 2003.

show abstract

Section: Expression Of Ictacalcin In Envelope Cells and Keratinocytesmentioning

confidence: 83%

Section: Expression Of Ictacalcin In Envelope Cells and Keratinocytesmentioning

confidence: 99%

Skin‐specific expression of ictacalcin, a homolog of the S100 genes, during zebrafish embryogenesis

Hsiao

Ekker

Tsai

2003

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

“…With this in mind, the expression of IFs, particularly keratins and vimentin, has been evaluated in a number of fishes representing various branches of the evolutionary tree. The fishes most extensively studied include the shark Scyliorhinus stellaris (Schaffeld et al 1998(Schaffeld et al , 2001, the zebrafish Danio rerio (Cerdá et al 1998;Conrad et al 1998;Schaffeld et al 2003), the rainbow trout Oncorhyncus mykiss (Markl and Franke 1988;Markl et al 1989;Herrmann et al 1996;Schaffeld et al 2002a,b), the common goldfish Carassius auratus (Giordano et al 1989(Giordano et al , 1990Cohen et al 1991;Druger et al 1992Druger et al , 1994, the carp Cyprinus carpio (Groff et al 1997a,b;Mencarelli and Cotelli 1997), and the lungfish Protopterus aethiopicus (Alibardi 2001(Alibardi , 2002Alibardi and Joss 2003;Schaffeld et al 2005). These investigations have shown that, in vertebrates generally, two keratin expression sets can be distiguished: "E" keratins are expressed in the epidermis and other stratified epithelia, and "S" keratins are present in internal simple epithelia.…”

Section: Introductionmentioning

confidence: 99%

“…These investigations have shown that, in vertebrates generally, two keratin expression sets can be distiguished: "E" keratins are expressed in the epidermis and other stratified epithelia, and "S" keratins are present in internal simple epithelia. The typical "S" keratins are represented by the heterodimeric pair K8/K18 that moreover seem to be the only keratins present in all examined vertebrate groups, including lamprey, shark, bony fish, and terrestrial vertebrates (e.g., Schaffeld et al 1998Schaffeld et al , 2002a.…”

Section: Introductionmentioning

confidence: 99%

Identification of keratins and analysis of their expression in carp and goldfish: comparison with the zebrafish and trout keratin catalog

et al. 2005

Self Cite

View full text Add to dashboard Cite

With more than 50 genes in human, keratins make up a large gene family, but the evolutionary pressure leading to their diversity remains largely unclear. Nevertheless, this diversity offers a means to examine the evolutionary relationships among organisms that express keratins. Here, we report the analysis of keratins expressed in two cyprinid fishes, goldfish and carp, by two-dimensional polyacrylamide gel electrophoresis, complementary keratin blot binding assay, and immunoblotting. We further explore the expression of keratins by immunofluorescence microscopy. Comparison is made with the keratin expression and catalogs of zebrafish and rainbow trout. The keratins among these fishes exhibit a similar range of molecular weights and isoelectric points, with a similar overall pattern on two-dimensional gels. In addition, immunofluorescence microscopy studies of goldfish and carp tissues have revealed the expression of keratins in both epithelial and mesenchymally derived tissues, as reported previously for zebrafish and trout. We conclude that keratin expression is qualitatively similar among these fishes, with goldfish and carp patterns being more similar to each other than to zebrafish, and the cyprinid fishes being more similar to each other than to the salmonid trout. Because of the detected similarity of keratin expression among the cyprinid fishes, we propose that, for certain experiments, they are interchangeable. Although the zebrafish distinguishes itself as being a developmental and genetic/genomic model organism, we have found that the goldfish, in particular, is a more suitable model for both biochemical and histological studies of the cytoskeleton, especially since goldfish cytoskeletal preparations seem to be more resistant to degradation than those from carp or zebrafish.

show abstract

“…The most recent works on keratins are from marine turtles, and were published by Lorenzo Alibardi and co-workers. Fish keratins, including those from sharks (Schaffeld et al 1998(Schaffeld et al , 2001(Schaffeld et al , 2004, were mostly investigated in terms of their molecular biology rather than for their bioinspirational potential (see for review Markl and Schechter 1998 ;Zimek et al 2003 ;Schaffeld and Markl 2004 ;Krushna Padhi et al 2006 ). Only fi sh with keratin-like fi brillar proteins, such as those from hagfi sh slime, have been intensively investigated in the Lab of Douglas Fudge as biological materials (represented below).…”

mentioning

confidence: 99%

Marine Keratins

Ehrlich

2014

Biological Materials of Marine Origin

View full text Add to dashboard Cite

Keratins represent the largest subgroup among all intermediate fi lament proteins. These structural proteins are mechanically robust and chemically unreactive. This is due to tight packing of protein chains in the form of alpha-helices or β-sheets into supercoiled polypeptide chains crosslinked with disulphide bonds. This chapter is dedicated to keratins of marine fi sh, birds, reptilian and mammalian origin. Keratin-like biological materials from hagfi sh slime are also discussed.

show abstract

Tracing keratin evolution: Catalog, expression patterns and primary structure of shark (Scyliorhinus stellaris) keratins

Cited by 29 publications

References 24 publications

Skin‐specific expression of ictacalcin, a homolog of the S100 genes, during zebrafish embryogenesis

Skin‐specific expression of ictacalcin, a homolog of the S100 genes, during zebrafish embryogenesis

Identification of keratins and analysis of their expression in carp and goldfish: comparison with the zebrafish and trout keratin catalog

Marine Keratins

Contact Info

Product

Resources

About