Tracing native and inoculated Rhizophagus irregularis in three potato cultivars (Charlotte, Nicola and Bintje) grown under field conditions

Buysens, Catherine; Alaux, Pierre-Louis; César, Vincent; Huret, Stéphanie; Declerck, Stéphane; Cranenbrouck, Sylvie

doi:10.1016/j.apsoil.2017.03.007

Cited by 14 publications

(8 citation statements)

References 43 publications

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“…Similarly to our results, the same AMF isolate inoculated on two genotypes of durum wheat was found at physiological maturity within crop roots, by tracing the SSU region [21]. Moreover, the inoculated R. irregularis MUCL41833 isolate was retrieved at harvest only in one over two potato cultivars, tracing a more discriminant gene (mitochondrial large ribosomal subunit, mtLSU RNA gene) [99]. Previous works, tracing the SSU RNA gene, the internal transcriber spacer 2 (ITS2) and the large subunit (LSU), successfully discriminated between inoculated and native isolates, and found in Medicago sativa the inoculated Funneliformis isolates AZ225C and IMA1 at early crop growth stages, whereas two years after inoculation only AZ225C was retrieved [42].…”

Section: Arbuscular Mycorrhizal Fungal Community Diversitysupporting

confidence: 86%

Field Inoculation of Bread Wheat with Rhizophagus irregularis under Organic Farming: Variability in Growth Response and Nutritional Uptake of Eleven Old Genotypes and A Modern Variety

et al. 2020

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Arbuscular mycorrhizal fungi (AMF) promote crop growth and yield by increasing N and P uptake and disease resistance, but the role of field AMF inoculation on the uptake of micronutrients, such as Fe and Zn, and accumulation in plant edible portions is still not clarified. Therefore, we studied the effect of field inoculation with Rhizophagus irregularis in an organic system on 11 old genotypes and a modern variety of bread wheat. Inoculation increased root colonization, root biomass and shoot Zn concentration at early stage and grain Fe concentration at harvest, while it did not modify yield. Genotypes widely varied for shoot Zn concentration at early stage, and for plant height, grain yield, Zn and protein concentration at harvest. Inoculation differentially modified root AMF community of the genotypes Autonomia B, Frassineto and Bologna. A higher abundance of Rhizophagus sp., putatively corresponding to the inoculated isolate, was only proved in Frassineto. The increase of plant growth and grain Zn content in Frassineto is likely linked to the higher R. irregularis abundance. The AMF role in increasing micronutrient uptake in grain was proved. This supports the introduction of inoculation in cereal farming, if the variable response of wheat genotypes to inoculation is considered.

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Section: Arbuscular Mycorrhizal Fungal Community Diversitysupporting

confidence: 86%

Field Inoculation of Bread Wheat with Rhizophagus irregularis under Organic Farming: Variability in Growth Response and Nutritional Uptake of Eleven Old Genotypes and A Modern Variety

et al. 2020

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“…It was commonly found in highly polluted environments (Hassan et al, 2011;Li et al, 2020). Rhizophagus is also a genus of AMF known for its adaptability and for being an R-strategist (Campagnac and Khasa, 2013;Sêdzielewska Toro and Brachmann, 2016;Buysens et al, 2017;Zhang et al, 2018;Cruz-Paredes et al, 2020;Lee et al, 2020). Members of the Rhizophagus genus have been commercialized and utilized as biofertilizers for more than three decades (Badri et al, 2016;Hijri, 2016;Basiru et al, 2021).…”

Section: Effects Of Rotation Systems On Arbuscular Mycorrhizal Fungi ...mentioning

confidence: 99%

Long-Term Persistence of Arbuscular Mycorrhizal Fungi in the Rhizosphere and Bulk Soils of Non-host Brassica napus and Their Networks of Co-occurring Microbes

et al. 2022

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Arbuscular mycorrhizal fungi (AMF) are obligate plant symbionts that improve the nutrition and health of their host. Most, but not all the crops form a symbiosis with AMF. It is the case for canola (Brassica napus), an important crop in the Canadian Prairies that is known to not form this association. From 2008 to 2018, an experiment was replicated at three locations of the Canadian Prairies and it was used to assess the impact of canola on the community of AMF naturally occurring in three cropping systems, canola monoculture, or canola in two different rotation systems (2-years, canola-wheat and 3-years, barley-pea-canola). We sampled canola rhizosphere and bulk soils to: (i) determine diversity and community structure of AMF, we expected that canola will negatively impact AMF communities in function of its frequency in crop rotations and (ii) wanted to assess how these AMF communities interact with other fungi and bacteria. We detected 49 AMF amplicon sequence variants (ASVs) in canola rhizosphere and bulk soils, confirming the persistence of a diversified AMF community in canola-planted soil, even after 10 years of canola monoculture, which was unexpected considering that canola is among non-mycorrhizal plants. Network analysis revealed a broad range of potential interactions between canola-associated AMF and some fungal and bacterial taxa. We report for the first time that two AMF, Funneliformis mosseae and Rhizophagus iranicus, shared their bacterial cohort almost entirely in bulk soil. Our results suggest the existence of non-species-specific AMF-bacteria or AMF-fungi relationships that could benefit AMF in absence of host plants. The persistence of an AMF community in canola rhizosphere and bulk soils brings a new light on AMF ecology and leads to new perspectives for further studies about AMF and soil microbes interactions and AMF subsistence without mycotrophic host plants.

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“…Rhizophagus irregularis BEG140 was reported to survive and proliferate up to 3 years in the field and was found more often in the inoculated than in control plots ( Sýkorová et al, 2012 ), while R. irregularis IR27 was reported to persist in jujube tree roots up to 18 months. More recently, Buysens et al (2017) , using a qPCR approach and amplifying the mtLSU RNA gene, performed for the first time the absolute quantification in the field of the isolate R. irregularis MUCL 41833, and found a significant correlation between mtLSU_MUCL41833 and total, arbuscular and vesicular colonization of roots of three varieties of potatoes at three growing stages (i.e., flowering, before defoliation and harvest). Finally, Kokkoris et al (2019) successfully traced the R. irregularis DAOM 197198 using specific primers, targeting the mt cox3-rnl intergenic mtDNA region, and a probe in a droplet digital PCR.…”

Section: Discussionmentioning

confidence: 99%

“…Despite the fact that a high rDNA sequence polymorphism was proved within individuals (e.g., Hijri et al, 1999;Lanfranco et al, 1999;Jansa et al, 2002;Thiéry et al, 2012), other researchers (Krüger et al, 2009(Krüger et al, , 2012Stockinger et al, 2010), similarly to our findings, proved that the combination of the highly polymorphic ITS region with variable SSU and LSU regions can be used to achieve phylogenetic species-and strain-level resolution in AMF. In this regard, Farmer et al (2007) could discriminate isolates of R. irregularis BEG141 and C. etunicatum BEG168, using only the variable domains of the LSU of ribosomal RNA gene, whereas more recent studies proved that other molecular markers (i.e., mtLSU, RPB1, and mt cox3-rnl intergenic mtDNA region) are even more efficient in the discrimination of foreign AM fungal isolates in the field (Sýkorová et al, 2012;Buysens et al, 2017;Kokkoris et al, 2019;Thioye et al, 2019). Very recently, to overcome the difficulties of the lack of consensus barcoding region for the determination of AM fungal taxa, Kolaříková et al (2021) developed a fine and promising approach to sequence an AM fungal marker within the ribosome-encoding operon (rDNA) which covers all the three widely applied variable molecular markers (SSU, ITS, and LSU).…”

Section: Molecular Discrimination Of Foreignmentioning

confidence: 99%

Multiple Arbuscular Mycorrhizal Fungal Consortia Enhance Yield and Fatty Acids of Medicago sativa: A Two-Year Field Study on Agronomic Traits and Tracing of Fungal Persistence

2022

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Arbuscular mycorrhizal fungi are promoted as biofertilizers due to potential benefits in crop productivity, and macro- and microelement uptake. However, crop response to arbuscular mycorrhizal fungi (AMF) inoculation is context-dependent, and AMF diversity and field establishment and persistence of inoculants can greatly contribute to variation in outcomes. This study was designed to test the hypotheses that multiple and local AMF inoculants could enhance alfalfa yield and fatty acids (FA) compared to exotic isolates either single or in the mixture. We aimed also to verify the persistence of inoculated AMF, and which component of the AMF communities was the major driver of plant traits. Therefore, a field experiment of AMF inoculation of alfalfa (Medicago sativa L.) with three single foreign isolates, a mixture of the foreign isolates (FMix), and a highly diverse mixture of local AMF (LMix) was set up. We showed that AMF improved alfalfa yield (+ 68%), nutrient (+ 147% N content and + 182% P content in forage), and FA content (+ 105%). These positive effects persisted for at least 2 years post-inoculation and were associated with enhanced AMF abundance in roots. Consortia of AMF strains acted in synergy, and the mixture of foreign AMF isolates provided greater benefits compared to local consortia (+ 20% forage yield, + 36% forage N content, + 18% forage P content, + 20% total FA in forage). Foreign strains of Funneliformis mosseae and Rhizophagus irregularis persisted in the roots of alfalfa 2 years following inoculation, either as single inoculum or as a component of the mixture. Among inoculants, F. mosseae BEG12 and AZ225C and the FMix exerted a higher impact on the local AMF community compared with LMix and R. irregularis BEG141. Finally, the stimulation of the proliferation of a single-taxa (R. irregularis cluster1) induced by all inoculants was the main determinant of the host benefits. Crop productivity and quality as well as field persistence of inoculated AMF support the use of mixtures of foreign AMF. On the other hand, local mixtures showed a lower impact on native AMF. These results pave the way for extending the study on the effect of AMF mixtures for the production of high-quality forage for the animal diet.

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Tracing native and inoculated Rhizophagus irregularis in three potato cultivars (Charlotte, Nicola and Bintje) grown under field conditions

Cited by 14 publications

References 43 publications

Field Inoculation of Bread Wheat with Rhizophagus irregularis under Organic Farming: Variability in Growth Response and Nutritional Uptake of Eleven Old Genotypes and A Modern Variety

Field Inoculation of Bread Wheat with Rhizophagus irregularis under Organic Farming: Variability in Growth Response and Nutritional Uptake of Eleven Old Genotypes and A Modern Variety

Long-Term Persistence of Arbuscular Mycorrhizal Fungi in the Rhizosphere and Bulk Soils of Non-host Brassica napus and Their Networks of Co-occurring Microbes

Multiple Arbuscular Mycorrhizal Fungal Consortia Enhance Yield and Fatty Acids of Medicago sativa: A Two-Year Field Study on Agronomic Traits and Tracing of Fungal Persistence

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