Tracing (r)bST in cattle: Liquid-based options for extraction and separation

Regal, Patricia; Lamas, Alexandre; Fente, C.; Franco, Carlos; Cepeda, Alberto

doi:10.1080/10826076.2017.1331453

Cited by 6 publications

(3 citation statements)

References 39 publications

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“…While methods for extracting rbGH from serum or commercial syringes usually involve a precipitation step with ammonium sulphate ((NH 4 ) 2 SO 4 ) [52], it can be anticipated that applying this strategy to milk would lead to the co-precipitation of the other proteins, in particular caseins, which are in the majority and will consequently carry the rbGH in their network, preventing its further extraction. Consequently, to deal with this protein problem, two options are possible: either the specific elimination of caseins by precipitation at their pI 4.6 before precipitating rbGH at its own pI (7.8) [10,53] or, in order to avoid these successive and sensitive precipitation steps, to extract rbGH directly from the milk, for example, by immunoaffinity, without trying to eliminate the interfering proteins [54].…”

Section: Proteinsmentioning

confidence: 99%

The Promise and Challenges of Determining Recombinant Bovine Growth Hormone in Milk

Raux

Bichon

Benedetto

et al. 2022

Foods

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Recombinant bovine growth hormone (rbGH) is produced in large quantities and widely used in a number of countries worldwide to stimulate milk production in dairy animals. The use of this compound in animal production is strictly regulated by food safety directives in force, in particular in the European Union (EU). Although analytical strategies for the detection of rbGH in blood have been successfully reported over the past 15 years, they do not fully answer the expectations of either competent authorities or industrials that would expect measuring its occurrence directly in the milk. As a matrix of excretion but also of consumption, milk appears indeed as the matrix of choice for detecting the use of rbGH in dairy animals. It also allows large volumes to be collected without presenting an invasive character for the animal. However, rbGH detection in milk presents several challenges, mainly related to the sensitivity required for its detection in a complex biological matrix. This review article presents the specific difficulties associated with milk and provides an overview of the analytical strategies reported in the literature and whether they concern indirect or direct approaches to the detection of rbGH administration to animals, with applications either for screening or confirmation purposes.

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Section: Proteinsmentioning

confidence: 99%

The Promise and Challenges of Determining Recombinant Bovine Growth Hormone in Milk

Raux

Bichon

Benedetto

et al. 2022

Foods

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“…Conversely, the administration of rbST to dairy animals is permitted in other countries, such as the USA, Brazil, and Mexico. In this context, several analytical methods have been developed to trace the presence of rbST in milk and other matrices and thus control its administration [ 16 ]. Confirmation of rbST (ab)use is not always easy since it requires tedious sample preparation, sophisticated analytical instruments, and very low detection limits.…”

Section: Introductionmentioning

confidence: 99%

Impact of Recombinant Bovine Somatotropin on Bovine Milk Composition and Fatty Acidome: A Multidose Longitudinal Study

Barreiro

Lamas

Miranda

et al. 2022

Foods

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Somatotropin is a species-specific polypeptide hormone produced in the pituitary gland of vertebrates. When administered exogenously to cattle, it can increase milk yield. However, the trade and administration of recombinant bovine somatotropin (rbST) to farm animals have been banned in the European Union (EU). Aside from food safety issues, very little is known about the effects of this hormone on milk composition and quality. In this work, a wide profile of fatty acids (the so-called fatty acidome) was determined by GC-FID in raw milk collected from control and rbST-treated lactating cows in a multidose longitudinal study. Milk composition (lactose, protein, fat, dry matter), including minerals (Ca, K, Mg, Na, P), was also determined, and milk yield was recorded. A tendency toward a less saturated profile was observed in the milk collected from animals treated with rbST, with higher concentrations of monounsaturated fatty acids. In addition, less calcium and potassium and more lactose and protein content were observed in milk from treated animals than in regular milk. As a result of this multicomponent profiling of milk, a clear impact of somatotropin treatment on milk quality was observed. The obtained results should be particularly interesting for those countries that permit the use of this hormone in dairy production.

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“…Furthermore, some methods were developed to detect rbST in bovine and buffalo serum, using liquid chromatography/tandem mass spectrometry [ 9 , 10 ]. However, due to different rbST variants available with varying terminal amino-acid compositions, and the low circulation levels of rbST in blood, it is difficult to detect rbST using these methods [ 11 ]. Therefore, the use of indirect methods could be an alternative for screening the use of rbST in cattle.…”

Section: Introductionmentioning

confidence: 99%

Tracing Recombinant Bovine Somatotropin Ab(Use) Through Gene Expression in Blood, Hair Follicles, and Milk Somatic Cells: A Matrix Comparison

et al. 2018

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The use of recombinant bovine somatotropin (rbST) in dairy cattle is forbidden in the European Union. Due to the very low circulating concentration of rbST in treated animals, its direct detection is still a challenge. Therefore, the use of indirect methods to detect the ab(use) of rbST in dairy cattle appears as a good alternative. In the past few years, gene expression demonstrated its utility in screening the use of illicit substances in both humans and animals. In this study, a comparison of three types of matrices (milk somatic cells, blood, and hair follicles) was carried out to evaluate their potential use for routine control of rbST using 15 gene-expression profiles. A total of six rbST-treated cows and three control cows were included in the study. A subcutaneous injection containing 500 mg of rbST was administered to the treated group. Samples of the three matrices were collected before rbST administration, and at three and nine days after treatment. The quality of RNA extracted was higher in the blood and hair-follicle samples than in the milk somatic cells. In the three matrices, there were significant differences in the expression of some genes, with milk somatic cells and blood presenting the the best matrices. On this note, the cyclin D1 (CCND1), interleukin 1 beta (IL-1β), tumor necrosis factor (TNF), and insulin-like growth factor 1 receptor (IGF-1R) genes showed potential as biomarkers of rbST treatment. Therefore, blood, somatic cells, and follicle hair should be considered as promising sources of RNA, and can be used in gene-expression assays to routinely control the illicit use of rbST.

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Tracing (r)bST in cattle: Liquid-based options for extraction and separation

Cited by 6 publications

References 39 publications

The Promise and Challenges of Determining Recombinant Bovine Growth Hormone in Milk

The Promise and Challenges of Determining Recombinant Bovine Growth Hormone in Milk

Impact of Recombinant Bovine Somatotropin on Bovine Milk Composition and Fatty Acidome: A Multidose Longitudinal Study

Tracing Recombinant Bovine Somatotropin Ab(Use) Through Gene Expression in Blood, Hair Follicles, and Milk Somatic Cells: A Matrix Comparison

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