Tracing upconversion nanoparticle penetration in human skin

Khabir, Zahra; Guller, Anna; Rozova, Vlada S.; Liang, Liuen; Lai, Yi-Jen; Goldys, Ewa M.; Hu, Honghua; Vickery, Karen; Zvyagin, Andrei V.

doi:10.1016/j.colsurfb.2019.110480

Cited by 14 publications

(5 citation statements)

References 56 publications

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“…Additionally, these studies can open new dimensions for the use of the nanoparticles; as they can be tailored/designed to provide skin healing and therapeutic effects, alongside their primary function which is the stabilization of the emulsion. Unlike the case of Pickering emulsions, tracking of the nanoparticles and investigating their skin penetration and distribution has been tackled by several studies in which the particles acted individually as dermal and/or transdermal delivery vehicles [28,[37][38][39]. In these studies, confocal laser scanning microscopy (CLSM) has proved to be a successful technique for the tracking of the particles.…”

Section: Introductionmentioning

confidence: 99%

New Pickering emulsions stabilized with chitosan/collagen peptides nanoparticles: Synthesis, characterization and tracking of the nanoparticles after skin application

Sharkawy

Barreiro

Rodrigues

2021

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Section: Introductionmentioning

confidence: 99%

New Pickering emulsions stabilized with chitosan/collagen peptides nanoparticles: Synthesis, characterization and tracking of the nanoparticles after skin application

Sharkawy

Barreiro

Rodrigues

2021

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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“…Until now the permeation of metallic US-NPs (Au, Ag, NaYF 4 : Yb/Er) across the skin has been measured by different techniques: optical and electron microscopy, − X-ray fluorescence spectrometry (XRF), inductively coupled plasma mass spectrometry (ICP-MS), inductively coupled plasma optical emission spectrometer (ICP-OES), and laser ablation inductively coupled plasma-mass spectrometry (LAICP-MS) . Most of these techniques either cannot be physically coupled to Franz diffusion cells to measure in real-time the passage of US-NPs across the skin (e.g., XRF, optical, and electron microscopy), or they appear to be too limited in their sensitivity (e.g., ICP-MS and ICP-OES).…”

Section: Introductionmentioning

confidence: 99%

High-Sensitivity Permeation Analysis of Ultrasmall Nanoparticles Across the Skin by Positron Emission Tomography

et al. 2021

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Ultrasmall nanoparticles (US-NPs; <20 nm in hydrodynamic size) are now included in a variety of pharmacological and cosmetic products, and new technologies are needed to detect at high sensitivity the passage of small doses of these products across biological barriers such as the skin. In this work, a diffusion cell adapted to positron emission tomography (PET), a highly sensitive imaging technology, was developed to measure the passage of gold NPs (AuNPs) in skin samples in continuous mode. US-AuNPs (3.2 nm diam.; TEM) were functionalized with deferoxamine (DFO) and radiolabeled with 89Zr(IV) (half-life: 3.3 days, matching the timeline of diffusion tests). The physicochemical properties of the functionalized US-AuNPs (US-AuNPs-PEG-DFO) were characterized by FTIR (DFO grafting; hydroxamate peaks: 1629.0 cm–1, 1569.0 cm–1), XPS (presence of the OC–N C 1s peak of DFO at 287.49 eV), and TGA (organic mass fraction). The passage of US-AuNPs-PEG-DFO-89Zr(IV) in skin samples was measured by PET, and the diffusion parameters were extracted thereby. The signals of radioactive US-AuNPs-PEG-DFO-89Zr(IV) leaving the donor compartment, passing through the skin, and entering the acceptor compartment were detected in continuous at concentrations as low as 2.2 nM of Au. The high-sensitivity acquisitions performed in continuous allowed for the first time to extract the lag time to the start of permeation, the lag time to start of the steady state, the diffusion coefficients, and the influx data for AuNPs permeating into the skin. PET could represent a highly valuable tool for the development of nanoparticle-containing topical formulations of drugs and cosmetics.

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“…The iWO-DCL was performed by our original immersion DCL method [ 36 , 60 ] with some modifications. The extracted CE organs, including the livers, lungs, hearts, ventriculi and proventriculi, brains, small intestines, spleens, breast muscles, and skins, were washed in sterile PBS and placed in 50 mL Falcone tubes, with 5–15 organs/per tube (depending on the organ’s size, for example, up to 5 livers per tube) filled with 35 mL of 0.1% solution of sodium dodecyl sulphate (SDS) in phosphate-buffered saline (PBS), then closed tightly and fixed horizontally on the platform of an orbital shaker.…”

Section: Methodsmentioning

confidence: 99%

“…Spontaneous (derived from the transplanted tumors) or genetically engineered animal models of metastases possess many limitations linked to the contribution of the immunocompromised microenvironment, the animal origin of the tumors, or the time course of the metastatic disease development. Overall, animal modeling is not ideal for the micrometastases simulation due to the low throughput capacity [ 36 ] (vs. a high cost). This is especially notable in drug development research, where large and reproducible numbers of representative colonies are needed.…”

Section: Introductionmentioning

confidence: 99%

Chick Embryo Experimental Platform for Micrometastases Research in a 3D Tissue Engineering Model: Cancer Biology, Drug Development, and Nanotechnology Applications

et al. 2021

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Colonization of distant organs by tumor cells is a critical step of cancer progression. The initial avascular stage of this process (micrometastasis) remains almost inaccessible to study due to the lack of relevant experimental approaches. Herein, we introduce an in vitro/in vivo model of organ-specific micrometastases of triple-negative breast cancer (TNBC) that is fully implemented in a cost-efficient chick embryo (CE) experimental platform. The model was built as three-dimensional (3D) tissue engineering constructs (TECs) combining human MDA-MB-231 cells and decellularized CE organ-specific scaffolds. TNBC cells colonized CE organ-specific scaffolds in 2–3 weeks, forming tissue-like structures. The feasibility of this methodology for basic cancer research, drug development, and nanomedicine was demonstrated on a model of hepatic micrometastasis of TNBC. We revealed that MDA-MB-231 differentially colonize parenchymal and stromal compartments of the liver-specific extracellular matrix (LS-ECM) and become more resistant to the treatment with molecular doxorubicin (Dox) and Dox-loaded mesoporous silica nanoparticles than in monolayer cultures. When grafted on CE chorioallantoic membrane, LS-ECM-based TECs induced angiogenic switch. These findings may have important implications for the diagnosis and treatment of TNBC. The methodology established here is scalable and adaptable for pharmacological testing and cancer biology research of various metastatic and primary tumors.

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Tracing upconversion nanoparticle penetration in human skin

Cited by 14 publications

References 56 publications

New Pickering emulsions stabilized with chitosan/collagen peptides nanoparticles: Synthesis, characterization and tracking of the nanoparticles after skin application

New Pickering emulsions stabilized with chitosan/collagen peptides nanoparticles: Synthesis, characterization and tracking of the nanoparticles after skin application

High-Sensitivity Permeation Analysis of Ultrasmall Nanoparticles Across the Skin by Positron Emission Tomography

Chick Embryo Experimental Platform for Micrometastases Research in a 3D Tissue Engineering Model: Cancer Biology, Drug Development, and Nanotechnology Applications

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