Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe

Ali, Firoj; Aute, Sunil; Sreedharan, Sreejesh; Anila, H A; Saeed, Hiwa K.; Smythe, Carl; Thomas, Jim A.; Das, Amitava

doi:10.1039/c7cc09433g

Cited by 36 publications

(13 citation statements)

References 35 publications

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“…It also takes charge of the synthesis of other molecules, like lipids and carbohydrates, as well as Ca 2+ storage 30b,96. During synthesis of diverse proteins, RONSS are indispensable for controlling their functional three-dimensional structures and chemical groups 50b,97. Therefore, maintaining suitable oxidative microenvironments is essential for ER functions.…”

Section: Endoplasmic Reticulum Targeted Probesmentioning

confidence: 99%

Fluorescent probes for organelle-targeted bioactive species imaging

et al. 2019

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The dynamic fluctuations of bioactive species in living cells are associated with numerous physiological and pathological phenomena. The emergence of organelle-targeted fluorescent probes has significantly facilitated our understanding on the biological functions of these species. This review describes the design, applications, challenges and potential directions of organelle-targeted bioactive species probes.

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Section: Endoplasmic Reticulum Targeted Probesmentioning

confidence: 99%

Fluorescent probes for organelle-targeted bioactive species imaging

et al. 2019

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“…[66][67][68] A much broader application potential presents the detection and quantifying of certain molecular or ionic analytes upon introduction of a fluorescence quenching process by defined substituents. This allows monitoring of analytes such as glucagon, 69 thiol, 61 nitric oxide, 53 hypochlorous acid, 70 nitroxyl, 71 fluoride, 72 and various metal cations such as Cd 2+ , 54 K + , Rb + , Cs + and Ba 2+ , 55 Cu 2+ and Hg 2+ . [56][57][58] From all reported sensor systems, the introduction of a PET-based fluorescence quenching process often showed the best performances with respect to sensitivity and signal enhancement.…”

Section: Bodipys For Sensing Applicationsmentioning

confidence: 99%

Simple Self-Referenced Luminescent pH Sensors Based on Upconversion Nanocrystals and pH-Sensitive Fluorescent BODIPY Dyes

et al. 2019

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Optical probes for monitoring, imaging, and sensing of pH are of great interest for the scientific community as pH is a crucial marker for many processes in biotechnology, biology, medical diagnostics, biomedical research, and material corrosion. Thereby, optical pH sensors based on fluorescence have attracted interest in particular as fluorescence offers a high sensitivity down to the single molecule level, can be read out with relatively simple and readily miniaturized instrumentation, and allows online in situ measurements. Also the versatility ranging from molecular and nanosensor formats to planar optodes and fiber-optic sensors, and the non-invasive, non-destructive, and contactless nature of the measurement are application-friendly features. The information content, which is offered by a fluorescence intensity-based sensor, is usually unspecific and limited on the presence or the absence of the chromophore or analyte and can additionally be hampered by fluctuation of the excitation light intensity and changes in fluorophore concentration, e.g., due to photobleaching. Therefore, many fluorescence sensors are utilized in referenced systems, which enable twowavelength ratiometric measurements of the fluorescence intensity by the introduction of an analyte-inert reference with a spectrally distinguishable emission. This work presents the rational design of a versatile, modular, multi-component-based platform for ratiometric optical analyte sensing that can be simply adapted to different formats and measurement geometries. Therefore, readily available analyte-responsive fluorescent boron-dipyrromethene (BODIPY) dyes and near infrared (NIR)-excitable multicolour-Partikelgrößen wurden dafür via Transmissionselektronenmikroskopie (TEM) und Kleinwinkel-Röntgenstreuung (SAXS) bestimmt. Neben der Partikelgröße konnten durch die TEM-Messungen auch Informationen über die Kristallphasen der Nanopartikel erhalten werden. Neben der Erfassung des Partikelwachstums wurde die UCL der UCNPs für die ratiometrische Sensorplattform als Nanolampe und gleichzeitig als Referenzsignal verwendet. Die blaue Upconversion(UC)-Emission der NaYF 4 :Yb 3+ /Tm 3+ UCNPs wurde dabei zur Anregung der pH-sensitiven BODIPY-Farbstoffe verwendet, während die rote UC-Emission als inertes Referenzsignal verwendet wurde. Die Berechnung des Verhältnisses der Emissionsintensitäten der grünen Fluoreszenz des Farbstoffs und der roten UC-Emission des Partikels ermöglicht eine Bestimmung des pH-Werts. Das Potenzial dieser Strategie zur Erfassung des pH-Werts wurde beispielhaft für die Bestimmung der zeitabhängigen Änderungen des pH-Werts einer metabolisierenden Escherichia coli (E. coli)-Suspension gezeigt.

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Section: Resultsmentioning

confidence: 99%

Recent Advances in Fluorescent Probes for Detection of HOCl and HNO

et al. 2020

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It is known that reactive oxygen (ROS) and nitrogen (RNS) species play a diverse role in various biological processes, such as inflammation, signal transduction, and neurodegenerative injury, apart from causing various diseases caused by oxidative and nitrosative stresses, respectively, by ROS and RNS. Thus, it is very important to quantify the concentration level of ROS and RNS in live cells, tissues, and organisms. Various smallmolecule-based fluorescent/chemodosimetric probes are reported to quantify and map the effective distribution of ROS/RNS under in vitro/in vivo conditions with a great spatial and temporal resolution. Such reagents are now appreciated as an excellent tool for aiding breakthroughs in modern redox biology. This minireview is a brief, but all-inclusive, account of such molecular probes that have been developed recently.

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Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe

Cited by 36 publications

References 35 publications

Fluorescent probes for organelle-targeted bioactive species imaging

Fluorescent probes for organelle-targeted bioactive species imaging

Simple Self-Referenced Luminescent pH Sensors Based on Upconversion Nanocrystals and pH-Sensitive Fluorescent BODIPY Dyes

Recent Advances in Fluorescent Probes for Detection of HOCl and HNO

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