Tracking Mercury in Individual <i>Tetrahymena</i> Using a Capillary Single-Cell Inductively Coupled Plasma Mass Spectrometry Online System

Shi, Jianbo; Ji, Xiaomeng; Wu, Qi; Liu, Hongwei; Qu, Guangbo; Yin, Yongguang; Hu, Ligang; Jiang, Guibin

doi:10.1021/acs.analchem.9b03719

Cited by 36 publications

(16 citation statements)

References 34 publications

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“…The iterative algorithm data processing strategy is widely used for MS data processing in single-cell analysis. ,− The iterative calculation for the whole data set through the 3 times standard deviation (3σ) finds the signal threshold, and all bursts with an intensity higher than this threshold are collected and considered as the nanoparticle or cellular events. This approach is convenient for data processing in the situation that one data point represents a single-cell signal (single-cell event).…”

Section: Results and Discussionmentioning

confidence: 99%

Two-Dimensional Cytometry Platform for Single-Particle/Cell Analysis with Laser-Induced Fluorescence and ICP–MS

Wei

Men

et al. 2021

Anal. Chem.

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A two-dimensional cytometry platform (CytoLM) with high sensitivity and high temporal resolution is developed for single-particle and single-cell sampling and analysis. First, a Dean flow-assisted vortex capillary cell sampling (VCCS) unit confines the sample stream in curved flow and drives to focus and align the particles or cells in a small probe volume. By coupling VCCS to a laser-induced fluorescence (LIF) detector with data acquisition and processing capability, a high-throughput single-particle/cell analysis system (VCCS–LIF) was established. The particle analysis throughput of 119.42/s and a detection recovery of 78.20 ± 1.75% were achieved at a density of 9.16 × 104/mL for fluorescent particles, and the cell analysis throughput is 48.20/s at a density of 1.5 × 105/mL. Second, the CytoLM platform is constructed by hyphenating VCCS–LIF with inductively coupled plasma mass spectrometry (ICP–MS). In the analysis of HepG2 cells by Ag+ incubation and AO staining, 10,760 fluorescence bursts and 3068 MS events were observed in 240 s. Invalid signals due to undispersed cells were controlled at 3.80% for LIF and 1.01% for MS, with a proportion of effective signal of >96.20%. After peak identification and integral processing of the original data, the statistical results including peak area, height, width, and spacing are obtained concurrently and the information on concentration and elemental quantification of single cells is evaluated. CytoLM facilitates high-throughput, multi-dimensional, and multi-parameter characterization of particles and cells, and it may provide vast potential in life science analysis.

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Section: Results and Discussionmentioning

confidence: 99%

Two-Dimensional Cytometry Platform for Single-Particle/Cell Analysis with Laser-Induced Fluorescence and ICP–MS

Wei

Men

et al. 2021

Anal. Chem.

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“…Eighteen (18) fish samples from sampling sites S 8 and S 9 in the Asunafo South district of the Tano Basin were studied between November 2016 and October 2017. e levels of seven trace metals mercury (Hg), cadmium (Cd), lead (Pb), copper (Cu), arsenic (As), zinc (Zn), and chromium (Cr) in the muscles of fish samples from River Tano were studied. e selection of these metals was based on their toxicity, medical importance, bioaccumulation, and persistence [28]. e study area was mapped and georeferenced with the aid of a hand-held Garmin 62SC Geographical Positioning System appliance [12].…”

Section: Study Design and Samplingmentioning

confidence: 99%

“…Ingestion of fish polluted with high levels of Hg, As, and Cr is associated with health problems, including kidney, liver, and skin cancers [1,12,26]. For example, the Minamata disease in Japan in the 60s was attributed to methylmercury (MeHg) exposure through seafood consumption [28].…”

Section: Heavy Metals In the Muscles Of Fish Samples From Rivermentioning

confidence: 99%

Heavy Metal Concentrations in Fish from River Tano in Ghana and the Health Risks Posed to Consumers

Nyantakyi

Wiafe

Akoto

et al. 2021

Journal of Environmental and Public Health

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Appreciable levels of trace metals have been reported in the Tano basin, but data on the corresponding levels in fish and the risk they pose to consumers are limited. The levels of 7 trace metals in 18 fish muscles were assessed between November 2016 and October 2017 using acid digestion and PerkinElmer (PinAACle 900T) Atomic Absorption Spectrophotometry. Apart from Cu, all the metals studied were detected in all fish samples. The levels of Cr, As, and Hg were higher than the acceptable levels of fish muscles. Cr concentration ranged from 16.10 ± 0.2 mg/kg in Clarias gariepinus to 57.9 ± 4.2 mg/kg in Sarotherodon galilaeus. The levels of As ranged from 1.01 ± 0.08 in Clarias gariepinus to 3.00 ± 0.01 mg/kg in Mormyrus rume. Hg level was 0.58 ± 0.69 mg/kg in Oreochromis niloticus and 2.52 ± 0.70 mg/kg in Ctenopoma kingsleyae. However, Pb, Zn, and Cd concentrations were below the Food and Agriculture Organization limits with low target hazard quotients in all fish samples, suggesting no possible noncarcinogenic risks to adult consumers. Possible noncarcinogenic and carcinogenic health risks were recorded for As, Hg, and Cr in all fish species. Strong associations were observed between Hg, As, Zn, and Cr and between Pb and Cd suggesting a possible common source. Mormyrus rume fish species was under stress in the river, but the remaining species were in good condition. Periodic monitoring of trace metal concentrations in fish and enforcement of the buffer zone policy are recommended.

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“…In contrast to the soft ion sources, ICP is a high-temperature ion source in which all the chemical bonds in a sample (e.g., a cell) are broken and the generated elemental isotopes are ionized efficiently, thus making ICP–MS a powerful method for ultratrace elemental analysis . Trace elements, − metal drugs, , and metal NPs , in single-cell suspensions have been successfully analyzed with ICP–MS. By element-tagged antibody labeling of single cells, ICP–MS equipped with a time-of-flight mass analyzer enables the simultaneous determination of more than 30 markers (antigens) in a single cell in suspension …”

mentioning

confidence: 99%

Single-Cell Isotope Dilution Analysis with LA–ICP–MS: A New Approach for Quantification of Nanoparticles in Single Cells

et al. 2020

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Laser ablation–inductively coupled plasma–mass spectrometry (LA–ICP–MS) is an emerging method for the analysis of metal nanoparticles (NPs) in single cells. However, two main obstacles, low analytical throughput and lack of commercial reference materials, need to be overcome. In this work, we demonstrated the principles of a new approach termed “single-cell isotope dilution analysis” (SCIDA) to remove the two obstacles. For a proof of concept, macrophage cells were chosen as a model to study the uptake of silver NPs (AgNPs) at a single-cell level. Single cells exposed to AgNPs were placed in an array by a microfluidic technique; each cell in the array was precisely dispensed with a known picoliter droplet of an enriched isotope solution with a commercial inkjet printer; accurate quantification of AgNPs in single cells was done by using isotope dilution LA–ICP–MS. The average Ag mass of 1100 single cells, 396 ± 219 fg Ag per cell, was in good accord with the average of the population of cells determined by solution ICP–MS analysis. The detection limit was 0.2 fg Ag per cell. The SCIDA approach is expected to be widely applied for the study of cell–NP interactions and biological effects of NPs at the single-cell level.

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Tracking Mercury in Individual Tetrahymena Using a Capillary Single-Cell Inductively Coupled Plasma Mass Spectrometry Online System

Cited by 36 publications

References 34 publications

Two-Dimensional Cytometry Platform for Single-Particle/Cell Analysis with Laser-Induced Fluorescence and ICP–MS

Two-Dimensional Cytometry Platform for Single-Particle/Cell Analysis with Laser-Induced Fluorescence and ICP–MS

Heavy Metal Concentrations in Fish from River Tano in Ghana and the Health Risks Posed to Consumers

Single-Cell Isotope Dilution Analysis with LA–ICP–MS: A New Approach for Quantification of Nanoparticles in Single Cells

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