Tracking of Listeria monocytogenes in meat establishment using Whole Genome Sequencing as a food safety management tool: A proof of concept

Nastasijević, Ivan; Milanov, Dubravka; Velebit, Branko; Djordjević, Vesna; Swift, Craig; Painset, Anaïs; Lakicević, Brankica

doi:10.1016/j.ijfoodmicro.2017.06.015

Cited by 54 publications

(44 citation statements)

References 28 publications

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“…Nastasijevic et al . () applied single nucleotide polymorphism analysis to WGS data to determine the genetic relatedness of strains and trace contamination through a meat production facility to a single line (slaughter line) demonstrating that the use of subtyping techniques enables source tracking through a food processing environment. Identification of persistent strains and contamination sources would enable companies who operate in the FLPSC to employ a ‘seek and destroy’ strategy (Stasiewicz et al .…”

Section: Source Tracking L Monocytogenes In the Flpscmentioning

confidence: 99%

“…Leong et al (2017) used PGFE to subtype isolates from a variety of food processing facilities and were able to not only identify persistent strains in vegetable processing facilities which subsequently contaminated produce but also recognize that elucidation of the specific source of contamination in a processing facility requires sampling over a longer time. Nastasijevic et al (2017) applied single nucleotide polymorphism analysis to WGS data to determine the genetic relatedness of strains and trace contamination through a meat production facility to a single line (slaughter line) demonstrating that the use of subtyping techniques enables source tracking through a food processing environment. Identification of persistent strains and contamination sources would enable companies who operate in the FLPSC to employ a 'seek and destroy' strategy (Stasiewicz et al 2015) to eradicate the contaminating bacteria from contaminated environments.…”

Section: Source Tracking L Monocytogenes In the Flpscmentioning

confidence: 99%

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Sources and survival ofListeria monocytogeneson fresh, leafy produce

Smith

Moorhouse²,

Monaghan

et al. 2018

J Appl Microbiol

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Listeria monocytogenes is an intracellular human pathogen which enters the body through contaminated food stuffs and is known to contaminate fresh leafy produce such as spinach, lettuce and rocket. Routinely, fresh leafy produce is grown and processed on a large scale before reaching the consumer through various products such as sandwiches and prepared salads. From farm to fork, the fresh leafy produce supply chain (FLPSC) is complex and contains a diverse range of environments where L. monocytogenes is sporadically detected during routine sampling of produce and processing areas. This review describes sources of the bacteria in the FLPSC and outlines the physiological and molecular mechanisms behind its survival in the different environments associated with growing and processing fresh produce. Finally, current methods of source tracking the bacteria in the context of the food supply chain are discussed with emphasis on how these methods can provide additional, valuable information on the risk that L. monocytogenes isolates pose to the consumer.

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Section: Source Tracking L Monocytogenes In the Flpscmentioning

confidence: 99%

Section: Source Tracking L Monocytogenes In the Flpscmentioning

confidence: 99%

Sources and survival ofListeria monocytogeneson fresh, leafy produce

Smith

Moorhouse²,

Monaghan

et al. 2018

J Appl Microbiol

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“…L. monocytogenes is present in natural ecosystems and is widely disseminated in different environmental niches [2]. It has been isolated from humans, and more than 50 species of wild and domestic animals, including mammals, birds, fish, crustaceans, and ticks, in addition to environmental sources, such as animal silage, soil, plants, sewage, stream water, and processing environments [3][4][5][6]. Although the genus Listeria includes many species, due to the pathogenicity of L. monocytogenes in human hosts and its ability to flourish in hostile environments, earlier research implicating genome sequencing were to a great extent concentrated on L. monocytogenes [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Prevalence, Pathogenicity, Virulence, Antibiotic Resistance, and Phylogenetic Analysis of Biofilm-Producing Listeria monocytogenes Isolated from Different Ecological Niches in Egypt: Food, Humans, Animals, and Environment

Osman

Kappell

Fox³

et al. 2019

Pathogens

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Serious outbreaks of foodborne disease have been caused by Listeria monocytogenes found in retail delicatessens and the severity of disease is significant, with high hospitalization and mortality rates. Little is understood about the formidable public health threat of L. monocytogenes in all four niches, humans, animals, food, and environment, in Egypt. This study analyzed the presence of L. monocytogenes collected from the four environmental niches and bioinformatics analysis was implemented to analyze and compare the data. PCR was used to detect virulence genes encoded by pathogenicity island (LIPI-1). prfA amino acid substation that causes constitutive expression of virulence was common in 77.7% of isolates. BLAST analysis did not match other isolates in the NCBI database, suggesting this may be a characteristic of the region associated with these isolates. A second group included the NH1 isolate originating in China, and BLAST analysis showed this prfA allele was shared with isolates from other global locations, such as Europe and North America. Identification of possible links and transmission pathways between the four niches helps to decrease the risk of disease in humans, to take more specific control measures in the context of disease prevention, to limit economic losses associated with food recalls, and highlights the need for treatment options.

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“…This report describes Salmonella as the commonest detected agent, and the highest-risk agent/food pairs the Salmonella in eggs and meat and meat products, and the analysis of strong-evidence foodborne outbreaks is associated with animal origin food [2]. In the case of meat products with longer shelf life, bacteria have more time to grow if they have the conditions (such as cooked sausages, cooked sliced ham and fermented salami) [3].…”

Section: Introductionmentioning

confidence: 99%

High Hydrostatic Pressure Treatment of Meat Products

Garcı́a-Gimeno¹,

Izquierdo²

2020

Food Processing

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High hydrostatic pressure (HHP) treatment has been described to improve the microbiological safety and shelf life of ready-to-eat (RTE) meat products, as a nonthermal decontamination technology in the meat industry, applied at pre-or post-packaging. The pathogen widely studied in this product is Listeria monocytogenes that reflects the concern of the food industry. In general, microorganism's lethality during HHP treatment depends on specific intrinsic factors of the microorganism; those factors are related to food and technological factors of treatment. In addition to processing parameters, intrinsic factors of the food matrix also exert an effect on bacteria inactivation during pressure treatment. It is known that low water activity (a w) protects microorganisms against the effects of pressure. Predictive modelling is an important tool of the novel microbial food safety management strategy that provides with accurate information to demonstrate and guarantee the safety and shelf life of the food products. The chapter describes the effect of parameters on the efficiency of this technology on meat products over pathogens, composition and the sensorial quality consequences. The predictive modelling tool is introduced for the optimisation of meat treatment.

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Tracking of Listeria monocytogenes in meat establishment using Whole Genome Sequencing as a food safety management tool: A proof of concept

Cited by 54 publications

References 28 publications

Sources and survival ofListeria monocytogeneson fresh, leafy produce

Sources and survival ofListeria monocytogeneson fresh, leafy produce

Prevalence, Pathogenicity, Virulence, Antibiotic Resistance, and Phylogenetic Analysis of Biofilm-Producing Listeria monocytogenes Isolated from Different Ecological Niches in Egypt: Food, Humans, Animals, and Environment

High Hydrostatic Pressure Treatment of Meat Products

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