Tracking Osteoarthritis Progress through Cationic Nanoprobe-Enhanced Photoacoustic Imaging of Cartilage

Xiao, Shuyi; Tang, Yufu; Lin, Yimu; Lv, Zhuang; Chen, Liang

doi:10.1016/j.actbio.2020.04.001

Cited by 20 publications

(15 citation statements)

References 63 publications

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“…Precipites were resuspended a final time in superpure water and stored at 4 °C. DMs were sterilized via cobalt 60 (60Co) irradiation (Oliveira et al, 2019 ; Shi et al, 2020 ; Lainović et al, 2020 ; Xiao et al, 2020 ; Liu et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

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Synergic fabrication of pembrolizumab loaded doxorubicin incorporating microbubbles delivery for ultrasound contrast agents mediated anti-proliferation and apoptosis

Liu

Chen

et al. 2021

Drug Delivery

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This study evaluated pembrolizumab-conjugated, doxorubicin (DOX)-loaded microbubbles (PDMs) in combination with ultrasound (US) as molecular imaging agents for early diagnosis of B cell lymphomas, and as a targeted drug delivery system. Pembrolizumab, a monoclonal CD20 antibody, was attached to the surfaces of DOX-loaded microbubbles. PDM binding to B cell lymphoma cells was assessed using immunofluorescence. The cytotoxic effects of PDMs in combination with ultrasound (PDMs þ US) were evaluated in vitro in CD20þ and CD20-cell lines, and its antitumor activities were assessed in Raji (CD20þ) and Jurkat (CD20-) lymphoma cell-grafted mice. PDMs specifically bound to CD20þ cells in vitro and in vivo. Contrast enhancement was monitored in vivo via US. PDM peak intensities and contrast enhancement durations were higher in Raji than in Jurkat cell-grafted mice (p < 0.05). PDMs þ US treatment resulted in improved antitumor effects and reduced systemic toxicity in Raji cell-grafted mice compared with other treatments (p < .05). Our results showed that PDMs þ US enhanced tumor targeting, reduced systemic toxicity, and inhibited CD20þ B cell lymphoma growth in vivo. Targeted PDMs could be employed as US molecular imaging agents for early diagnosis, and are an effective targeted drug delivery system in combination with US for CD20þ B cell malignancy treatment.

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Section: Methodsmentioning

confidence: 99%

“…The superpure water wash was repeated several times until the supernatant become transparent. Precipites were resuspended a final time in superpure water and stored at 4 C. DMs were sterilized via cobalt 60 (60Co) irradiation (Oliveira et al, 2019;Shi et al, 2020;Lainovi c et al, 2020;Xiao et al, 2020;.…”

Section: Dm Preparationmentioning

confidence: 99%

Synergic fabrication of pembrolizumab loaded doxorubicin incorporating microbubbles delivery for ultrasound contrast agents mediated anti-proliferation and apoptosis

Liu

Chen

et al. 2021

Drug Delivery

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“…As sound scatters 1000 times less than light, the acoustic signal propagates much longer in biological tissue without significant attenuation [50]. This provides a nondestructive, real-time 3D imaging modality capable of evaluating changes in the tissue and detecting diseased states such as OA [51,52].…”

Section: Glossarymentioning

confidence: 99%

Nondestructive testing of native and tissue-engineered medical products: adding numbers to pictures

Castro

Babakhanova

et al. 2022

Trends in Biotechnology

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Traditional destructive tests are used for quality assurance and control within manufacturing workflows. Their applicability to biomanufacturing is limited due to inherent constraints of the biomanufacturing process. To address this, photo-and acoustic-based nondestructive testing has risen in prominence to interrogate not only structure and function, but also to integrate quantitative measurements of biochemical composition to cross-correlate structural, compositional, and functional variances. We survey relevant literature related to single-mode and multimodal nondestructive testing of soft tissues, which adds numbers (quantitative measurements) to pictures (qualitative data). Native and tissue-engineered articular cartilage is highlighted because active biomanufacturing processes are being developed. Included are recent efforts and prominent trends focused on technologies for clinical and in-process biomanufacturing applications. Nondestructive testing of soft tissuesNondestructive (see Glossary) qualitative and quantitative assessment of native and tissueengineered soft tissues is a research area with great clinical and industrial potential. Specific to the biomanufacturing of tissue-engineered medical products (TEMPs), a core challenge of the field is in understanding and assessing the critical quality attributes of engineered tissues and correlating the critical process parameters and material attributes that greatly affect the intended clinical function [1]. Therefore, there is a growing need to develop nondestructive and noninvasive quantitative tools and methods to verify and to validate the critical biochemical components of TEMPs produced from a biological source, monitor extracellular matrix (ECM) development and composition, and correlate these critical components to tissue function (continuously and at predefined timepoints) [1].For example, when developing TEMPs for articular cartilage (AC), the mechanical properties (function) of native tissue are used to qualify the robustness and project the long-term efficacy of developing and clinically used products [2]. One method of tissue engineering AC is using the self-assembling process, where mature chondrocytes are harvested, dedifferentiated, and subsequently redifferentiated in vitro to produce a more hyaline-like AC [3][4][5]. Tissue engineering is inherently time-, resource-, and cost-intensive, and the destructive testing used to verify and validate tissue-engineered AC can be inappropriate or impractical for scale-up and biomanufacturing. Therefore, nondestructive methods to assess the evolution of mechanical properties and correlate them back to structure and composition are needed to further the clinical translation of TEMPs.The structure and composition of tissues has been independently evaluated by linear and nonlinear optics-based imaging techniques (e.g., second-harmonic generation (SHG) [6][7][8][9], Highlights Optics-based nondestructive techniques have shown to produce corroborative and congruent results to traditional tests.

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“…At the early point of time (2 h), the existence of folate as a targeting ligand greatly enhanced the NIR signal from inflamed tissue. In another study, Shuyi et al [112] designed a cartilage-targeted cationic nanoprobe to enhance photoacoustic imaging (PAI) based on poly-l-lysine-melanin (PLL-M)-NPs to track the development of arthritis. The in vitro analysis displayed the capability of PLL-M-NPs to identify various high-sensitivity anionic glycosaminoglycan (GAG) concentrations.…”

Section: Nanoimagingmentioning

confidence: 99%

Nanomaterials for the Diagnosis and Treatment of Inflammatory Arthritis

Hosseinikhah

Barani

Rahdar

et al. 2021

IJMS

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Nanomaterials have received increasing attention due to their unique chemical and physical properties for the treatment of rheumatoid arthritis (RA), the most common complex multifactorial joint-associated autoimmune inflammatory disorder. RA is characterized by an inflammation of the synovium with increased production of proinflammatory cytokines (IL-1, IL-6, IL-8, and IL-10) and by the destruction of the articular cartilage and bone, and it is associated with the development of cardiovascular disorders such as heart attack and stroke. While a number of imaging tools allow for the monitoring and diagnosis of inflammatory arthritis, and despite ongoing work to enhance their sensitivity and precision, the proper assessment of RA remains difficult particularly in the early stages of the disease. Our goal here is to describe the benefits of applying various nanomaterials as next-generation RA imaging and detection tools using contrast agents and nanosensors and as improved drug delivery systems for the effective treatment of the disease.

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Tracking Osteoarthritis Progress through Cationic Nanoprobe-Enhanced Photoacoustic Imaging of Cartilage

Cited by 20 publications

References 63 publications

Synergic fabrication of pembrolizumab loaded doxorubicin incorporating microbubbles delivery for ultrasound contrast agents mediated anti-proliferation and apoptosis

Synergic fabrication of pembrolizumab loaded doxorubicin incorporating microbubbles delivery for ultrasound contrast agents mediated anti-proliferation and apoptosis

Nondestructive testing of native and tissue-engineered medical products: adding numbers to pictures

Nanomaterials for the Diagnosis and Treatment of Inflammatory Arthritis

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