Tracking protein domain movements by EPR distance determination and multilateration

Dance with spins: site-directed spin labeling coupled to electron paramagnetic resonance spectroscopy directly inside cells

“…Furthermore, the combination of sitedirected mutagenesis, DEER experiments and multilateration approaches make it possible to use experimental data for tracking movements of whole domains of macromolecules with respect to each other. 30,31 The upcoming challenge of the EPR community is to adapt the progress achieved in ''in vitro'' studies to ''in-cell'' ones. Indeed, the dynamics of biomolecules can be deeply influenced by the complexity of the cell, where transient aspecific interactions, sublocalization and environmental changes occur.…”

Insights into the Conformational Plasticity of the Protein Kinase Akt1 by Multi‐Lateral Dipolar Spectroscopy

“…A set of distance distributions between reference sites within a domain not involved in conformational changes and a labelled site within the domain of interest can be used to localize the latter (Figure 1). [27] As DEER gives not only a mean distance but a full distance distribution, the result of the multilateration is a spatially resolved distribution probability. So not only the location of maximal probability of the site of interest can be resolved, but also the degree of conformational flexibility of that site.…”