Tracking the environmental dissemination of carbapenem-resistant Klebsiella pneumoniae using whole genome sequencing

Ekwanzala, Mutshiene Deogratias; Dewar, John Barr; Kamika, Ilunga; Momba, Maggy Ndombo Benteke

doi:10.1016/j.scitotenv.2019.06.533

Cited by 35 publications

(37 citation statements)

References 69 publications

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“…Our study reveals for the first time the presence of carbapenemases-producing K. pneumoniae ST35, ST219, ST364, ST395, ST485 and ST1878 in wastewaters, of which ST395 has clinical importance, while ST35 and ST485 are sporadically related to clinical cases. The other STs, with major clinical significance, have already been described in wastewaters: ST11 [5,41,42,43], ST17 [44], ST45 [45], ST101 [43,46,47,48], ST258 [49], ST307 [45,49,50,51], ST512 [52], and the minor clone ST1564 [45].…”

Section: Discussionmentioning

confidence: 97%

Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania

Surleac

Barbu

Paraschiv³

et al. 2020

PLoS ONE

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We report on the genomic characterization of 47 multi-drug resistant, carbapenem resistant and ESBL-producing K. pneumoniae isolates from the influent (I) and effluent (E) of three wastewater treatment plants (WWTPs) and from Romanian hospital units which are discharging the wastewater in the sampled WWTPs. The K. pneumoniae whole genome sequences were analyzed for antibiotic resistance genes (ARGs), virulence genes and sequence types (STs) in order to compare their distribution in C, I and E samples. Both clinical and environmental samples harbored prevalent and widely distributed ESBL genes, i.e. bla SHV , bla OXA , bla TEM and bla CTX M . The most prevalent carbapenemase genes were bla NDM-1 , bla OXA-48 and bla KPC-2 . They were found in all types of isolates, while bla OXA-162 , a rare bla OXA-48 variant, was found exclusively in water samples. A higher diversity of carbapenemases genes was seen in wastewater isolates. The aminoglycoside modifying enzymes (AME) genes found in all types of samples were aac(6'), ant(2'') Ia, aph(3'), aaD, aac(3) and aph(6). Quinolone resistance gene qnrS1 and the multi-drug resistance oqxA/B pump gene were found in all samples, while qnrD and qnrB were associated to aquatic isolates. The antiseptics resistance gene qacEdelta1 was found in all samples, while qacE was detected exclusively in the clinical ones. Trimethroprim-sulfamethoxazole (dfrA, sul1 and sul2), tetracyclines (tetA and tetD) and fosfomycin (fosA6, known to be located on a transpozon) resistance genes were found in all samples, while for choramphenicol and macrolides some ARGs were detected in all samples (catA1 and catB3 / mphA), while other (catA2,

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Section: Discussionmentioning

confidence: 97%

Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania

Surleac

Barbu

Paraschiv³

et al. 2020

PLoS ONE

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“…Thus, its assessment should shed light on the transfer of antibiotics from WWTP to agricultural soils. On the other hand, when it comes to RS, recent reports have highlighted a high occurrence of resistant bacteria and genes [ 26 , 27 ]. Knowing that riverbed sediment can resuspend and re-enter the water column [ 34 ], it is important to assess such a vital component of the RW system.…”

Section: Discussionmentioning

confidence: 99%

“…Samples collected as part of a 24-h composite sample were collected on arrival at the municipal wastewater plant from the IW, AS, and EW compartments. For the sampling of HW and RW, a method previously used by Ekwanzala et al [ 26 , 27 ] was followed, where one sample consisted of a composite of five grab samples collected at 30-min intervals for better environmental representativeness of the matrix sampled. RW samples were collected within 100 m of the WWTP outlet (EW).…”

Section: Methodsmentioning

confidence: 99%

Environmental Dissemination of Selected Antibiotics from Hospital Wastewater to the Aquatic Environment

et al. 2020

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The environmental dissemination of selected antibiotics from hospital wastewater into municipal wastewater and lastly to a receiving water body was investigated. Selected antibiotics (azithromycin (AZM), ciprofloxacin (CIP), clindamycin (CDM), doxycycline (DXC) and sulfamethoxazole (SMZ)) present in effluents of academic hospital wastewater, influents, sewage sludge, and effluents of municipal wastewater, receiving water, and its benthic sediment samples were quantified using the Acquity® Waters Ultra-Performance Liquid Chromatography System hyphenated with a Waters Synapt G2 coupled to a quadrupole time-of-flight mass spectrometer. The overall results showed that all assessed antibiotics were found in all matrices. For solid matrices, river sediment samples had elevated concentrations with mean concentrations of 34,834, 35,623, 50,913, 55,263, and 41,781 ng/g for AZM, CIP, CDM, DXC, and SMZ, respectively, whereas for liquid samples, hospital wastewater and influent of wastewater had the highest concentrations. The lowest concentrations were observed in river water, with mean concentrations of 11, 97, 15, and 123 ng/L, except for CDM, which was 18 ng/L in the effluent of wastewater. The results showed that the highest percentages of antibiotics removed was SMZ with 90%, followed by DXC, AZM and CIP with a removal efficiency of 85%, 83%, and 83%, respectively. The antibiotic that showed the lowest removal percentage was CDM with 66%. However, the calculated environmental dissemination analysis through the use of mass load calculations revealed daily release of 15,486, 14,934, 1526, 922, and 680 mg/d for SMZ, CIP, AZM, DXC, and CDM, respectively, indicating a substantial release of selected antibiotics from wastewater to the river system, where they are possibly adsorbed in the river sediment. Further research into the efficient removal of antibiotics from wastewater and the identification of antibiotic sources in river sediment is needed.

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“…If they occur in an unchanged form, they may cause the risk of typical side effects after they enter the body. One group of drugs often identified in environmental samples are antibiotics, which may be responsible for the development of antibiotic-resistant bacteria [21]. As in biological samples, pharmaceuticals are present in the environment in very low concentrations.…”

Section: Introductionmentioning

confidence: 99%

The Influence of Ionic Liquids on the Effectiveness of Analytical Methods Used in the Monitoring of Human and Veterinary Pharmaceuticals in Biological and Environmental Samples—Trends and Perspectives

et al. 2020

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Recent years have seen the increased utilization of ionic liquids (ILs) in the development and optimization of analytical methods. Their unique and eco-friendly properties and the ability to modify their structure allows them to be useful both at the sample preparation stage and at the separation stage of the analytes. The use of ILs for the analysis of pharmaceuticals seems particularly interesting because of their systematic delivery to the environment. Nowadays, they are commonly detected in many countries at very low concentration levels. However, due to their specific physiological activity, pharmaceuticals are responsible for bioaccumulation and toxic effects in aquatic and terrestrial ecosystems as well as possibly upsetting the body’s equilibrium, leading to the dangerous phenomenon of drug resistance. This review will provide a comprehensive summary of the use of ILs in various sample preparation procedures and separation methods for the determination of pharmaceuticals in environmental and biological matrices based on liquid-based chromatography (LC, SFC, TLC), gas chromatography (GC) and electromigration techniques (e.g., capillary electrophoresis (CE)). Moreover, the advantages and disadvantages of ILs, which can appear during extraction and separation, will be presented and attention will be given to the criteria to be followed during the selection of ILs for specific applications.

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Tracking the environmental dissemination of carbapenem-resistant Klebsiella pneumoniae using whole genome sequencing

Cited by 35 publications

References 69 publications

Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania

Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania

Environmental Dissemination of Selected Antibiotics from Hospital Wastewater to the Aquatic Environment

The Influence of Ionic Liquids on the Effectiveness of Analytical Methods Used in the Monitoring of Human and Veterinary Pharmaceuticals in Biological and Environmental Samples—Trends and Perspectives

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