Tracking translation of single mRNA molecule in live cells

Wang, Wenjuan; Chen, Chunlai

doi:10.1007/s11434-016-1116-9

Cited by 1 publication

(1 citation statement)

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“…This kind of interactions and hence diffusions have different impact on the (1) rates of the translation (Klumpp et al 2013), (2) distribution of ribosomal mRNA (Chou 2003), (3) fraction of active translating mRNAs (Wang et al 2016a), (4) diffusion models of translation complexes in cells (Cottrell et al 2012), and (5) ribosome stalling (Dao Duc and Song 2018). With the advancement of methodology, several interesting aspects of translational dynamics of single mRNAs have been discovered, such as (a) the transition between translational and non-translational states of mRNA happens in a longer time scale, indicating mRNA may undergo through several translational shutdowns and re-initiation within their lifetime (Yan et al 2016); and (b) tracking real-time movements of mRNA-ribosome complexes expressing cytosolic proteins experience three different behaviors: stationary, subdiffusive, and diffusive (Wang and Chen 2016). Katz et al (2016) showed that mRNA co-moving with ribosome demonstrates slower diffusion features in the thousands of singleparticle trajectories compared to the non-translational mRNAs that are exposed to the translational inhibitors.…”

Section: Anomalous Diffusion In Gene Expressionmentioning

confidence: 99%

Understanding biochemical processes in the presence of sub-diffusive behavior of biomolecules in solution and living cells

2019

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In order to maintain cellular function, biomolecules like protein, DNA, and RNAs have to diffuse to the target spaces within the cell. Changes in the cytosolic microenvironment or in the nucleus during the fulfillment of these cellular processes affect their mobility, folding, and stability thereby impacting the transient or stable interactions with their adjacent neighbors in the organized and dynamic cellular interior. Using classical Brownian motion to elucidate the diffusion behavior of these biomolecules is hard considering their complex nature. The understanding of biomolecular diffusion inside cells still remains elusive due to the lack of a proper model that can be extrapolated to these cases. In this review, we have comprehensively addressed the progresses in this field, laying emphasis on the different aspects of anomalous diffusion in the different biochemical reactions in cell interior. These experiment-based models help to explain the diffusion behavior of biomolecules in the cytosolic and nuclear microenvironment. Moreover, since understanding of biochemical reactions within living cellular system is our main focus, we coupled the experimental observations with the concept of sub-diffusion from in vitro to in vivo condition. We believe that the pairing between the understanding of complex behavior and structure-function paradigm of biological molecules would take us forward by one step in order to solve the puzzle around diseases caused by cellular dysfunction.

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