Tracking Yeast Metabolism and the Crabtree Effect in Real Time via CO2 Production using Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS)

Ahmed, M. Rizwan; Doyle, Nicholas A.; Connolly, Cathal; McSweeney, Sean; Krüse, Jacob; Morrissey, John P.; Prentice, Michael B.; Fitzpatrick, Dara

doi:10.1016/j.jbiotec.2019.11.016

Cited by 3 publications

(5 citation statements)

References 21 publications

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“…Plenty of microorganisms can produce gas when growing in the products, causing swells in the packaging that vary from almost flat to severely swollen (Evancho et al., 2009). Concerning this problem, yeasts can utilize sugars in either anaerobic or aerobic ways with regard to their environmental condition, and eventually, they produce CO 2 (Ahmed et al., 2020). Yeasts are known as one of the huge groups among microorganisms that cause spoilage in fermented dairy products, such as doogh, in addition to soft drinks and juices via metabolic byproducts, namely acids and CO 2 , and for instance, K. lactis , S. cerevisiae , and Geotrichum species create froth and swell in doogh and yogurt packaging (Afzali et al., 2020).…”

Section: Resultsmentioning

confidence: 99%

Antifungal activity of protective cultures against the yogurt drink‐specific spoilage yeasts

Safdarianghomsheh

Majdinasab

Razmjooei

et al. 2022

Journal of Food Science

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Fungal agents emerged as post-pasteurization contamination are responsible for the spoilage in yogurt drink. In this work, the antifungal effects of some lactic acid bacteria (LAB) on the spoilage yeasts isolated from yogurt drink (Doogh) were evaluated. First, the microbial growth in the yogurt drink samples during the storage time was investigated, and the isolated microorganisms were identified using biochemical methods and sequencing of the specific amplicons. Yeasts (3-7 log CFU ml -1 ) were found to be the most abundant microorganisms (specific spoilage organisms) in several samples. Using the amplification technique of rDNA by ITS1 and ITS4 primers, the dominant yeasts were identified as Pichia kudriavzevii, Kluyveromyces marxianus, and Candida parapsilosis. Then, the antimicrobial activity of 37 strains of LAB against the isolated yeasts was studied using broth microdilution. Eventually, the strains of Lacticplantibacillus plantarum (245, 24, P6, and P7), Lactiplantibacillus pentosus (20), and Levilactobacillus brevis (30) exhibited significant antifungal activity. In the most effective impacts, lag times of C. parapsilosis, K. marxianus, and P. kudriavzevii were increased by almost 12-19 h, 12-19 h, and 2-6 h, respectively, while the area under the growth curve for these yeasts was reduced to lower than 40%, near 16%, and approximately 67%, in the order given. Overall, these bacteria showed high potential as the substituents for chemical preservatives in yogurt drinks.

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Section: Resultsmentioning

confidence: 99%

Antifungal activity of protective cultures against the yogurt drink‐specific spoilage yeasts

Safdarianghomsheh

Majdinasab

Razmjooei

et al. 2022

Journal of Food Science

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“…The genuine dissolution profile returned to steady state at 157 s while the falsified sample returned to steady state at 282 s. BARDS data is highly reproducible and the average standard deviation across spectral data points of triplicate measurements is ~ 0.122 kHz. This allows for a reduction in replication to duplicate measurements with an average % variance of ~ 2.0% 30 , 31 . The data of both samples in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In a typical BARDS analysis, the spectrometer records the steady state resonances of the system as a background reference for 30 s, before the sample is added and the stirrer is set in motion. The pitch of the resonance modes in the solution change significantly when the sample formulation is added before gradually returning to steady state over several minutes 30 , 31 . The received samples were of different formulations.…”

Section: Methodsmentioning

confidence: 99%

Sounding out falsified medicines from genuine medicines using Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS)

Alfarsi

Caillet

Fawbert

et al. 2021

Sci Rep

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The trade in falsified medicine has increased significantly and it is estimated that global falsified sales have reached $100 billion in 2020. The EU Falsified Medicines Directive states that falsified medicines do not only reach patients through illegal routes but also via the legal supply chain. Falsified medicines can contain harmful ingredients. They can also contain too little or too much active ingredient or no active ingredient at all. BARDS (Broadband Acoustic Resonance Dissolution Spectroscopy) harnesses an acoustic phenomenon associated with the dissolution of a sample (tablet or powder). The resulting acoustic spectrum is unique and intrinsic to the sample and can be used as an identifier or signature profile. BARDS was evaluated in this study to determine whether a product is falsified or genuine in a rapid manner and at lower cost than many existing technologies. A range of genuine and falsified medicines, including falsified antimalarial tablets from south-east Asia, were tested, and compared to their counterpart genuine products. Significant differences between genuine and falsified doses were found in their acoustic signatures as they disintegrate and dissolve. Principal component analysis was employed to differentiate between the genuine and falsified medicines. This demonstrates that the tablets and capsules included here have intrinsic acoustic signatures which could be used to screen the quality of medicines.

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“…Ahmed et al 37 reported BARDS data to be highly reproducible, and the average standard deviation across spectral data points of triplicate measurements was 0.122 kHz. This allows for a reduction in replication to duplicate measurements with an average variance of 2.06%.…”

Section: ■ Basics Of a Bards Responsementioning

confidence: 99%

“…The solution is at its most compressible at this point. Equation can also be expressed as eq : A detailed and comprehensive outline on the principles and processes involved in BARDS analysis is given by Fitzpatrick et al − …”

Section: Basics Of a Bards Responsementioning

confidence: 99%

Tracking Compression Changes in an Aqueous Electrolyte for Real-Time H₂ and O₂ Gas Evolution Quantification during Total Water Splitting Using BARDS

Kang

Alkhraije

McSweeney³

et al. 2020

ACS Appl. Energy Mater.

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Hydrogen fuel cell technology has the potential for integration with renewable energy sources to produce electricity without the need for fossil fuels. Efforts are being made in producing cheap and effective electrodes from new materials to make hydrogen production more efficient. Gas evolution, in all cases, requires an accurate analysis of electrochemical behavior of electrodes to quantify efficiency, improvement, or stability. Knowing the exact gas volume by any method in real time during electrochemical water splitting is urgently needed. Taking inspiration from the existing technique broadband acoustic resonance dissolution spectroscopy (BARDS), we demonstrate an original approach to continuously track the electrochemical water splitting via the gas volume evolution from hydrogen evolution reactions (HER) and oxygen evolution reactions (OER) processes. The technique may be used to unravel the true features of new electrode materials that evolve hydrogen and correlate material electrochemistry to the true gas volume evolved in real time.

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Tracking Yeast Metabolism and the Crabtree Effect in Real Time via CO2 Production using Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS)

Cited by 3 publications

References 21 publications

Antifungal activity of protective cultures against the yogurt drink‐specific spoilage yeasts

Antifungal activity of protective cultures against the yogurt drink‐specific spoilage yeasts

Sounding out falsified medicines from genuine medicines using Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS)

Tracking Compression Changes in an Aqueous Electrolyte for Real-Time H₂ and O₂ Gas Evolution Quantification during Total Water Splitting Using BARDS

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Tracking Yeast Metabolism and the Crabtree Effect in Real Time via CO2 Production using Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS)

Cited by 3 publications

References 21 publications

Antifungal activity of protective cultures against the yogurt drink‐specific spoilage yeasts

Antifungal activity of protective cultures against the yogurt drink‐specific spoilage yeasts

Sounding out falsified medicines from genuine medicines using Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS)

Tracking Compression Changes in an Aqueous Electrolyte for Real-Time H2 and O2 Gas Evolution Quantification during Total Water Splitting Using BARDS

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Product

Resources

About

Tracking Compression Changes in an Aqueous Electrolyte for Real-Time H₂ and O₂ Gas Evolution Quantification during Total Water Splitting Using BARDS