Trade-Offs Associated with Photoprotective Green Fluorescent Protein Expression as Potential Drivers of Balancing Selection for Color Polymorphism in Reef Corals

Quick, Cathryn; D’Angelo, Cecilia; Wiedenmann, Jörg

doi:10.3389/fmars.2018.00011

Cited by 23 publications

(38 citation statements)

References 64 publications

(124 reference statements)

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“…Having stabilized the eGFP-AA into a mixture of TMPE:PMMA in both gel and film forms, we turned to validate our initial hypothesis that FP motion triggered by constant irradiation is responsible for the heat generation in HLEDs. Following works on intracellular thermometry in cells [43][44][45][46][47] and living organism like corals 48,49 , where it is shown that the viscosity of the media is key to control the heat generation, we first analyzed the increase in temperature of eGFP-AA gels with different TMPE:PMMA mass ratios (see SI for more details). The maximum temperature reduced from 52°C to 40°C, to 32°C, and to 30°C upon increasing the viscosity of the gels for 1:1, 1:2, 1:4, and 1:6 mass ratios, respectively (Fig.…”

Section: Fluorescent Protein Designmentioning

confidence: 99%

“…In this context, this work presents a zero-thermal quenching FP-polymer phosphor, for which both, thermal-and photoinduced degradation processes and optical losses, are simultaneously circumvented embedding FPs into light-guiding and rigid hydrophobic host polymers. This biophosphor is inspired by previous observations about the role of green fluorescent proteins (GFPs) in (i) in vivo measurements of intracellular temperature combining temperature-induced molecular Brownian dynamics and polarized light excitation sources [43][44][45][46][47] and (ii) body temperature control observed in living organisms like corals, which involves the mechanical change of the stiffness of the soft tissue where GFP clusters are embedded 48,49 . Like in single cells, vibrational and rotational motions of FPs might also be operative in elastomeric coatings due to the low viscosity of TMPE that partially replaces the water surrounding the FP's surface.…”

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confidence: 99%

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Long-living and highly efficient bio-hybrid light-emitting diodes with zero-thermal-quenching biophosphors

et al. 2020

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Bio-hybrid light-emitting diodes (Bio-HLEDs) based on color down-converting filters with fluorescent proteins (FPs) have achieved moderate efficiencies (50 lm/W) and stabilities (300 h) due to both thermal-and photo-degradation. Here, we present a significant enhancement in efficiency (~130 lm/W) and stability (>150 days) using a zero-thermalquenching bio-phosphor design. This is achieved shielding the FP surface with a hydrophilic polymer allowing their homogenous integration into the network of a light-guiding and hydrophobic host polymer. We rationalize how the control of the mechanical and optical features of this bio-phosphor is paramount towards highly stable and efficient Bio-HLEDs, regardless of the operation conditions. This is validated by the relationships between the stiffness of the FP-polymer phosphor and the maximum temperature reached under device operation as well as the transmittance of the filters and device efficiency.

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Section: Fluorescent Protein Designmentioning

confidence: 99%

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confidence: 99%

Long-living and highly efficient bio-hybrid light-emitting diodes with zero-thermal-quenching biophosphors

et al. 2020

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“…Cnidarian hosts possess multiple mechanisms for sheltering endosymbionts including photoprotective host chromoproteins (Dove, Hoegh-Guldberg, & Ranganathan, 2001;Quick, D'Angelo, & Wiedenmann, 2018;Salih, Larkum, Cox, Kuhl, & Hoegh-Guldberg, 2000;Smith, D'Angelo, Salih, & Wiedenmann, 2013) and other host-mediated modifications to the internal light environment, such as scattering by the coral skeleton (Enríquez, Méndez, & Prieto, 2005;Wangpraseurt, Larkum, Ralph, & Kühl, 2012). Work by Bhagooli, Baird, and Ralph (2008) found that multiple genera of 2008; Leutenegger et al, 2007;Salih et al, 2000;Smith et al, 2013).…”

Section: Stress Responses In the Free-living Statementioning

confidence: 99%

“…In hospite reduction of blue light is a function of host filtering and shifting of spectra via fluorescent proteins (Leutenegger et al, 2007;Quick et al, 2018;Salih et al, 2000;Smith et al, 2013), resulting in an order of magnitude reduction in the amount of blue light transmitted to deeper tissue layers in corals (Lichtenberg, Larkum, & Kühl, 2016). Sorek and Levy (2012) showed that blue light spectra increased mRNA expression of free-living Symiodiniaceae cryptochromes CRY1 and CRY2, but were downregulated when hosted in the coral Stylophora pistillata.…”

Section: In Hospite Disruption Of Blue Light Photoreceptionmentioning

confidence: 99%

Free‐living and symbiotic lifestyles of a thermotolerant coral endosymbiont display profoundly distinct transcriptomes under both stable and heat stress conditions

et al. 2019

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Reef‐building corals depend upon a nutritional endosymbiosis with photosynthetic dinoflagellates of the family Symbiodiniaceae for the majority of their energetic needs. While this mutualistic relationship is impacted by numerous stressors, warming oceans are a predominant threat to coral reefs, placing the future of the world's reefs in peril. Some Symbiodiniaceae species exhibit tolerance to thermal stress, but the in hospite symbiont response to thermal stress is underexplored. To describe the underpinnings of symbiosis and heat stress response, we compared in hospite and free‐living transcriptomes of Durusdinium trenchii, a pan‐tropical heat‐tolerant Symbiodiniaceae species, under stable temperature conditions and acute hyperthermal stress. We discovered that symbiotic state was a larger driver of the transcriptional landscape than heat stress. The majority of differentially expressed transcripts between in hospite and free‐living cells were downregulated, suggesting the in hospite condition is associated with the shutdown of numerous processes uniquely required for a free‐living lifestyle. In the free‐living state, we identified enrichment for numerous cell signalling pathways and other functions related to detecting and responding to a changing environment, as well as transcripts relating to mitosis, meiosis, and motility. In contrast, in hospite cells exhibited enhanced transcriptional activity for photosynthesis and carbohydrate transport as well as chromatin modifications and a disrupted circadian clock. Hyperthermal stress induced drastic alteration of transcriptional activity in hospite, suggesting symbiotic engagement with the host elicited an exacerbated stress response when compared to free‐living D. trenchii. Altogether, the dramatic differences in gene expression between in hospite and free‐living D. trenchii indicate the importance of considering symbiotic state in investigations of symbiosis and hyperthermal stress in Symbiodiniaceae.

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“…smaragdina and rustica belong to the same genetic pool, we tested the correlation between the symbiotic composition and the morphotypes mainly differentiated by FP and related pigments expression (Wiedenmann et al 1999;. These proteins may have photoprotective functions (Salih et al 2000;Gittins et al 2015) or they may enhance symbiont photosynthesis (Quick et al 2018), suggesting a possible correlation between morph ଏuorescence pattern and the symbiotic composition of the morphs, irrespective of genetic differentiation. To assess the genetic diversity of the A. viridis symbiont populations, that all belong to the unique temperate clade A (Savage et al 2002;Visram et al 2006;Forcioli et al 2011;Casado-Amezúa et al 2014), we genotyped the pooled Symbiodiniaceae from each single host and compared the obtained symbiotic compositions with one another in order to detect any differentiation among the different host morphs or lineages.…”

Section: A Similar Symbiotic Dinoଏagellate Composition Among the Morphsmentioning

confidence: 99%

The many faced symbiotic snakelocks anemone (Anemonia viridis, Anthozoa): host and symbiont genetic differentiation among colour morphs

et al. 2019

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How can we explain morphological variations in a holobiont? The genetic determinism of phenotypes is not always obvious and could be circumstantial in complex organisms. In symbiotic cnidarians, it is known that morphology or colour can misrepresent a complex genetic and symbiotic diversity. Anemonia viridis is a symbiotic sea anemone from temperate seas. This species displays different colour morphs based on pigment content and lives in a wide geographical range. Here, we investigated whether colour morph differentiation correlated with host genetic diversity or associated symbiotic genetic diversity by using RAD sequencing and symbiotic dinoଏagellate typing of 140 sea anemones from the English Channel and the Mediterranean Sea. We did not observe genetic differentiation among colour morphs of A. viridis at the animal host or symbiont level, rejecting the hypothesis that A. viridis colour morphs correspond to species level differences. Interestingly, we however identiଏed at least four independent animal host genetic lineages in A. viridis that differed in their associated symbiont populations. In conclusion, although the functional role of the different morphotypes of A. viridis remains to be determined, our approach provides new insights on the existence of cryptic species within A. viridis.

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Trade-Offs Associated with Photoprotective Green Fluorescent Protein Expression as Potential Drivers of Balancing Selection for Color Polymorphism in Reef Corals

Cited by 23 publications

References 64 publications

Long-living and highly efficient bio-hybrid light-emitting diodes with zero-thermal-quenching biophosphors

Long-living and highly efficient bio-hybrid light-emitting diodes with zero-thermal-quenching biophosphors

Free‐living and symbiotic lifestyles of a thermotolerant coral endosymbiont display profoundly distinct transcriptomes under both stable and heat stress conditions

The many faced symbiotic snakelocks anemone (Anemonia viridis, Anthozoa): host and symbiont genetic differentiation among colour morphs

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