Tradescantia stamen-hair system as an excellent botanical tester of mutagenicity: its responses to ionizing radiations and chemical mutagens, and some synergistic effects found

Ichikawa, Sadao

doi:10.1016/0027-5107(92)90096-k

Cited by 73 publications

(61 citation statements)

References 56 publications

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“…[1][2][3] One of the methods most widely used to detect genotoxic activity of chemical or physical agents is the somatic mutation test on Tradescantia staminal hairs (clone 4430). 4 This test is based on the fact that Tradescantia staminal hair cells are heterozygous for color (Aa). The dominant allele (A) accounts for the blue color and the recessive allele (a) is responsible for the pink color.…”

Section: Introductionmentioning

confidence: 99%

“…5,6 The pink mutation frequency in the stamen hairs is expressed as the number of the mutant events, either per hair (usually 10 3 hairs) or per hair-cell division (usually 10 4 hair-cell division). 4 One of the most recent tests for detecting genetic damage in cells is the alkaline comet assay. It allows quantification of low levels of DNA damage in individual cells, is very simple, and only requires only a few cells.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of genotoxic activity of maleic hydrazide, ethyl methane sulfonate, and N-nitroso diethylamine in Tradescantia

Alvarez-Moya¹,

Santerre-Lucas²,

Zúñiga‐González³

et al. 2001

Salud pública Méx

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and used in both pink mutation assay and comet assay using cellular nuclei from Tradescantia staminal hairs. The observation of staminal hair was realized along eight days (6-14) after treatment), flowers produced day 14 after treatment were utilized done according to Underbrink. In previous reports on plants, were comet assay was used, breaking cellular wall and separating by centrifugation gradient are necessary. Here, nuclei from staminal hairs were obtained by squashing the cells (is not necessary to utilize to break special procedure cellular wall), collected using a nylon mesh of 80Mm and next the comet assay was applied. Student's T test was the statistical test used for analyzing the comet assay data. Results. Both assays showed a great sensitivity to the studied mutagens. A relationship between the dose-pink event and the dose-tail length was evident. Even ResumenObjetivo. Evaluar la genotoxicidad de N-nitroso dietilamina (NDEA), hidrazida málica (MH) y etil metano sulfonato (EMS), en núcleos de Tradescantia (clona 4430) por medio de la prueba del cometa y de la prueba de mutación rosa, en los pelos estaminales de la misma planta. Material y métodos. Las plantas de Tradescantia (clon 4430) fueron obtenidas del Laboratorio de Citogenética y Mutagénesis del Centro de ciencias de la Atmósfera de la Universidad Nacional Autónoma de México, tratadas con NDEA a 1, 5, 10 mM, MH a 1, 5, 10 mM y EMS a 15, 30 y 45 mM, y utilizadas en la prueba de mutación rosa y en la del cometa, en núcleos celulares de los pelos estaminales. En la primera, la lectura de los pelos estaminales se realizó de acuerdo con el método de Underbrink. En otros estudios, que han aplicado la prueba del cometa en plantas, existe la necesidad de romper la pared celular y separar los núcleos por gradiente de centrifugación; en este caso, los núcleos de las células de los pelos estaminales fueron extraídos por aplastamiento sin aplicar un procedimiento especial para romper la pared, colectados por filtración en una malla de nylon y sometidos a la prueba del cometa. La prueba t de Student se usó para analizar los datos obtenidos. Resultados. Ambas pruebas presentaron una gran sensibilidad a los mutá-genos estudiados y hubo una relación evidente dosis-eventos rosa / longitud de la cauda. Aunque la prueba de mutación rosa en Tradescantia fue muy sensible a MH y EMS, no se

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of genotoxic activity of maleic hydrazide, ethyl methane sulfonate, and N-nitroso diethylamine in Tradescantia

Alvarez-Moya¹,

Santerre-Lucas²,

Zúñiga‐González³

et al. 2001

Salud pública Méx

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“…A number of plant bioassays have been identified for in situ environmental monitoring (Uhl et al 2003). The most common assays used for studying mutagenicity of various pollutants in plants are based on the detection of chromosomal aberrations in Allium cepa (Fiskesjo 1985), Tradescantia (Ichikawa 1992), Vicia faba (Kanaya et al 1994) plants are suitable for cytological analysis, and responses seen in plant tests are highly correlated with those seen in other biological systems, making the plant test good for evaluating the genotoxicity of environmental samples (Grant 1999, Sadowska et al 2001. Genotoxicity of herbicides has been reported by several researchers in several plants, such as by Boyle and Evans (1974) in Secale-cereale L., Tartar et al (2006) in Allium cepa L and Allium sativum L, and by Mansour (1984) in Vicia faba.…”

Section: Discussionmentioning

confidence: 99%

Biomonitoring of Genotoxic Effect of Glyphosate and Pendimethalin in <i>Vigna mungo</i> Populations

Singh

Srivastava

2014

CYTOLOGIA

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Summary Genotoxicological biomonitoring of Vigna mungo populations in the Banda district in Uttar Pradesh, sprayed with the herbicides glyphosate and pendimethalin, was done by studying their meiotic behavior and morphological characters and comparing them with untreated populations.The herbicides caused a change in the frequency of different meiotic phases at a given time, and also induced various anomalies. Disturbed polarity, spindle disturbance, bridges, and laggards were common in both the populations. Micronuclei were found in a large number of cells. Pollen fertility was less in the glyphosate-treated group as compared to the pendimethalin-treated and control groups. A comparative account of the correlation between percentage anomaly and pollen fertility as indicators of genotoxicity of the two herbicides has been described in this paper.

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“…This clone is a diploid hybrid (2n = 12) between a blue-flowered Tradescantia hirsutiflora Bush and a pink-flowered T. subacaulis Bush (Emmerling- Thompson and Nawrocky, 1980), thus is a blue/pink heterozygote. This clone has been frequently used in studies of somatic mutations in the stamen hairs induced by ionizing radiations and various chemicals as reviewed earlier (Schairer and Sautkulis, 1982;Ichikawa, 1992), and has been shown to be more sensitive to alkylating agents than other clones (Sparrow et al, 1974;Nauman et al, 1976;Ichikawa et al, 1993).…”

Section: Methodsmentioning

confidence: 99%

“…In fact, mutagenic synergisms among different mutagens have clearly been detected with Tradescantia stamen-hair system (Cebulska-Wasilewska et al, 1981;Ichikawa, 1992;Ichikawa et al, 1993;Shima and Ichikawa, 1994. The mutagenic synergisms detected so far are those among four monofunctional alkylating agents and X rays, i.e., between ethyl methanesulfonate (EMS) and X rays (CebulskaWasilewska et al, 1981;Shima and Ichikawa, 1994), methyl methanesulfonate (MMS) and X rays (Ichikawa, 1992;Ichikawa et al, 1993;Shima and Ichikawa, 1994), EMS and MMS (Shima and Ichikawa, 1994), dimethyl sulfate (DMS) and X rays and N-ethyl-N-nitrosourea (ENU) and X rays . Differences in pattern of the synergisms have also been reported (Shima and Ichikawa, 1994.…”

Section: Introductionmentioning

confidence: 99%

Antagonistic effects of ethyl methanesulfonate and maleic hydrazide in inducing somatic mutations in the stamen hairs of Tradescantia clone BNL 4430.

Xiao

Ichikawa

1998

Genes Genet. Syst.

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Mutagenic interaction between ethyl methanesulfonate (EMS; a monofunctional alkylating agent) and maleic hydrazide (MH; a promutagen activated into a mutagen in plants highly likely by peroxidase) was studied in the stamen hairs of Tradescantia clone BNL 4430, a blue/pink heterozygote. Since EMS has been shown to act synergistically with X rays in inducing mutations, and mutagenic synergisms have also been observed between X rays and MH by exposing to X rays before MH treatments, EMS and MH were expected to act synergistically at least by exposing to EMS before MH treatment. Three different combined treatments were conducted, i.e., by exposing for 4 h to 18.8 mM EMS 44 or 20 h before starting or 20 h after completing 1 mM MH treatments for 4 h. Unexpectedly, however, clear antagonistic effects in inducing somatic pink mutations were detected after all these combined treatments. Especially, the induced mutation frequency by exposing to EMS 44 h before the MH treatment was significantly lower than that induced by MH alone. The clear mutagenic antagonisms observed were thought to have resulted from EMS-caused inhibition of activation of MH by peroxidase, EMS ethylating and thus inactivating this enzyme or its precursors. Decreased peroxidase activities than those after treatments with MH alone were measured after two combined treatments, i.e., 12 h after the one exposing to EMS 44 h before MH and 24 h after the other exposing to EMS 20 h after MH, but the decreases were not large enough or their fluctuations were too large to judge them to be statistically significant. Comparisons of mutation frequencies induced by the combined treatments exposing to EMS before MH with those by MH alone suggest that there are some mechanisms (other than ethylation of peroxidase or its precursors) by which EMS suppresses the activation of MH.

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Tradescantia stamen-hair system as an excellent botanical tester of mutagenicity: its responses to ionizing radiations and chemical mutagens, and some synergistic effects found

Cited by 73 publications

References 56 publications

Evaluation of genotoxic activity of maleic hydrazide, ethyl methane sulfonate, and N-nitroso diethylamine in Tradescantia

Evaluation of genotoxic activity of maleic hydrazide, ethyl methane sulfonate, and N-nitroso diethylamine in Tradescantia

Biomonitoring of Genotoxic Effect of Glyphosate and Pendimethalin in <i>Vigna mungo</i> Populations

Antagonistic effects of ethyl methanesulfonate and maleic hydrazide in inducing somatic mutations in the stamen hairs of Tradescantia clone BNL 4430.

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