Traditional drink of blackcincau(Mesona palustrisBL)-basedwedang uwuhas immunomodulator on alloxan-induced diabetic rats

Abstract

Purpose This study aims to evaluate the immunomodulatory effect of traditional drink of black cincau-based wedang uwuh (WUB) on alloxan-induced diabetic rats. Design/methodology/approach WUB consists of dried herbs such as black cincau leaves (Mesona palustris BL), red ginger (Zingiber officinale Rosc), cloves (Syzgium aromaticum), sappan wood (Caesalpinia sappan Lin) and soursop leaves (Annona muricata). In this study, the rats were divided into five groups: normal control, diabetic control and three groups of WUB (WUB 13.5 mL/kg and WUB 27 mL/kg) or wedang uwuh commercial (WUC) treated diabetic groups. WUB or WUC was administered by gavage for three days after rats were confirmed diabetic induced by alloxan; these injections were continued for 28 days. At the end of the experiment, the spleen of rats was analyzed using flow cytometry. Data were analyzed by ANOVA followed by Tukey test using Minitab version 16.0. Findings This study showed that WUB significantly inhibited the expression of pro-inflammatory cytokines (interferon-Y [IFN-ɣ] and tumor necrosis factor-α [TNF-a]) and anti-inflammatory cytokines (interleukin 10 [IL-10] and transforming growth factor-β [TGF-ß]), and achieved a balance between pro-inflammatory and anti-inflammatory cytokines that were not significantly different from normal controls. WUB 27 was able to regulate the production of relative average cytokines IFN-ɣ (7.6 ± 3.5; p = 0.010), TNF-a (8.7 ± 2.4; p = 0.018), IL-10 (6.3 ± 2.4; p = 0.001) and TGF-ß (7.4 ± 2.1; p = 0.004) that was significantly different from diabetic control. This study’s results validate that the use of WUB can result in immunomodulatory activity in diabetic rats. Originality/value To the best of the authors’ knowledge, this is the first study on the immunomodulatory effect of WUB which is developed based on WUC; WUB has been used by Indonesian people as a functional beverage which acts as an immune booster and body warmer.

Polyphenols: immunonutrients tipping the balance of immunometabolism in chronic diseases

“…Regarding adaptive immunity, studies employing cafeteria dietinduced obesity as well as the alloxan-induced model of diabetes in rats have reported that PCs intake lowered the production of proinflammatory mediators including ILs, TNFa, IFNg and TGF-b by MLN and splenic lymphocytes (275,276). Contrasting to what is observed for macrophages, PCs seem to promote Treg cell recruitment, namely through elevation of Foxp3 gene expression (277).…”

Potensi Antibakteri Minuman Fungsional Tradisional Jawa (Wedang Uwuh) Berdasarkan Variasi Waktu Rebusan

“…Minuman ini terdiri atas berbagai rempah yang kaya antioksidan, yaitu kayu secang, kapulaga, pala, kayu manis, jahe, cengkih, daun pala, daun kayu manis, ranting cengkih, cengkih, dan daun cengkih. Kandungan senyawa flavonoid yang berfungsi sebagai antioksidan dan antibakteri banyak terdapat pada bagian daun dan batang secang (Widyaningsih et al, 2020;Palimbong et al, 2020). Minuman secang memiliki kandungan total fenol tertinggi dibandingkan minuman lainnya.…”

The influence of roselle-based wedang uwuh (Hibiscus sabdariffa L.) on SOD (superoxide dismutase) and MDA (malondialdehyde) of cigarette smoke-exposed rat

“…Roselle-based wedang uwuh (RWU) and commercial wedang uwuh (CWU) were prepared according to Widyaningsih et al (2020) by extracting 3.5 g RWU and CWU in 350 ml of boiling water for 5 minutes and then filtered.…”