We aimed at reporting the chemical constituents and antimicrobial activities of
Leea aequata
L., a traditional folk medicine used in Myanmar for the treatment of wounds and skin diseases. A new neolignan, (7
S
,8
R
)-9′-
O
-acetylcedrusin (
1
), a new lactam, (3
S
,4
S
)-4-chloro-3-hydroxypiperidin-2-one (
2
), along with 21 known compounds, including five lignans (
3
–
7
), four flavonoid glycosides (
8
–
11
), and others (
12
–
23
), were isolated from the ethanoic extract of the aerial parts of
L. aequata
. The structures of the new compounds were determined by NMR, MS, and ECD spectra. For all the antimicrobial tests of the 23 compounds, only 3,4,5-trihydroxybenzoic acid ethyl ester (
17
) showed weak inhibitory activities against
Escherichia coli
and
Salmonella enterica
subsp.
enterica
.
Graphical Abstract
Electronic supplementary material
The online version of this article (10.1007/s13659-019-0209-y) contains supplementary material, which is available to authorized users.