Traditional marketed meats as a reservoir of multidrug-resistant Escherichia coli

Guibert, Fernando; Espinoza, Kathya; Taboada-Blanco, Clara; Alonso, Carla A.; Oporto, Rosario; Castillo, Angie K.; Rojo-Bezares, Beatriz; López, María; Sáenz, Yolanda; Pons, Maria J.; Ruiz, Joaquim

doi:10.1007/s10123-023-00445-y

Cited by 4 publications

(2 citation statements)

References 76 publications

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“…It should also be noted that CTX-M-15, the dominant variant worldwide [40], was not very frequently identified in this study. Similarly, other studies in the area have described the same finding, suggesting that CTX-M-15 is not the predominant ESBL in Peru [37,41]. CTX-M-8G, a rare ESBL group, has been previously detected in dog feces in Northern Peru [38].…”

Section: Antibiotic Patternsupporting

confidence: 73%

Antimicrobial resistance and associated risk factors in Escherichia coli isolated from Peruvian dogs: A focus on extended-spectrum β-lactamases and colistin

Ventura,

Oporto-Llerena,

Espinoza

et al. 2024

Vet World

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Background and Aim: Established antimicrobial resistance (AMR) surveillance in companion animals is lacking, particularly in low-middle-income countries. The aim of this study was to analyze AMR and its risk factors in Escherichia coli isolated from dogs at two veterinary centers in Lima (Peru). Materials and Methods: Ninety dogs were included in the study. Antimicrobial susceptibility was established by disk diffusion, whereas microdilution was used to determine colistin susceptibility. Mechanisms related to extended-spectrum β-lactamases (ESBL) and colistin resistance were determined by polymerase chain reaction. Clonal relationships of colistin-resistant isolates were assessed by XbaI-pulsed-field gel electrophoresis. Results: Thirty-five E. coli strains were isolated. High levels of resistance to ampicillin (57.1%), nalidixic acid (54.3%), tetracycline (48.6%), and azithromycin (25.7%) were detected. Cephalosporin resistance levels were ≥20% and those for colistin were 14.3%. Twelve (34.2%) isolates were ESBL producers; of these, six blaCTX-M-55 (50.0%), 2 (16.6%) blaCTX-M-15, and 2 (16.6%) blaCTX-M-8-like genes were found. The five colistin-resistant isolates were clonally unrelated, with four of them presenting amino acid codon substitutions in the mgrB gene (V8A) or mutations in the mgrB promoter (a12g, g98t, and c89t). Furthermore, dog age, <6 years (p = 0.027) and raw diet (p = 0.054) were associated with resistance to a greater number of antibiotic families. Conclusion: Despite small number of samples included, the study found that dogs studied were carriers of multidrug-resistant E. coli, including last-resort antimicrobials, representing a public health problem due to close contact between dogs and humans. This issue suggests the need for larger studies addressed to design strategies to prevent the spread of resistant micro-organisms in small animal clinics and domestic settings. Keywords: antibiotic resistance, colistin, dogs, extended-spectrum β-lactamases, Peru, risk factor.

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Section: Antibiotic Patternsupporting

confidence: 73%

Antimicrobial resistance and associated risk factors in Escherichia coli isolated from Peruvian dogs: A focus on extended-spectrum β-lactamases and colistin

Ventura,

Oporto-Llerena,

Espinoza

et al. 2024

Vet World

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“…These expansive uses have led to an increasing isolation of quinolone-resistant microorganisms, resulting in a worldwide epidemic of quinolone resistance from the mid-1990s onward [ 1 ]. Subsequently, quinolone resistance levels, selection, mechanisms, and dispersion pathways have been largely and increasingly studied since the 1960s [ 21 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 ].…”

Section: Introductionmentioning

confidence: 99%

Unusual and Unconsidered Mechanisms of Bacterial Resilience and Resistance to Quinolones

Ruiz

2024

Life

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Quinolone resistance has been largely related to the presence of specific point mutations in chromosomal targets, with an accessory role of impaired uptake and enhanced pump-out. Meanwhile the relevance of transferable mechanisms of resistance able to protect the target of pump-out or inactivate quinolones has been increasingly reported since 1998. Nevertheless, bacteria have other strategies and mechanisms allowing them to survive and even proliferate in the presence of quinolones, which might be qualified as resistance or resilience mechanisms. These include decreasing levels of quinolone target production, transient amoeba protection, benthonic lifestyle, nutrient-independent slow growth, activation of stringent response, inactivation or degradation of quinolones as well as apparently unrelated or forgotten chromosomal mutations. These mechanisms have been largely overlooked, either because of the use of classical approaches to antibiotic resistance determination or due to the low increase in final minimum inhibitory concentration levels. This article is devoted to a review of a series of these mechanisms.

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Antibiotic-Resistant Pseudomonas aeruginosa from Market Meat in Peru

Guibert,

Rojo-Bezares,

Espinoza

et al. 2024

Foodborne Pathogens and Disease

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Traditional marketed meats as a reservoir of multidrug-resistant Escherichia coli

Cited by 4 publications

References 76 publications

Antimicrobial resistance and associated risk factors in Escherichia coli isolated from Peruvian dogs: A focus on extended-spectrum β-lactamases and colistin

Antimicrobial resistance and associated risk factors in Escherichia coli isolated from Peruvian dogs: A focus on extended-spectrum β-lactamases and colistin

Unusual and Unconsidered Mechanisms of Bacterial Resilience and Resistance to Quinolones

Antibiotic-Resistant Pseudomonas aeruginosa from Market Meat in Peru

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