Traditional Microalgae Isolation Techniques

Andersen, Robert A.; Kawachi, Masanobu

doi:10.1016/b978-012088426-1/50007-x

Cited by 386 publications

(275 citation statements)

References 28 publications

Supporting

Mentioning

197

Contrasting

Unclassified

Order By: Relevance

“…Agar plates is probably the oldest and the most used method of purification, but this method is effective only for algae that can grow either on the surface of agar or embedded in it [3,24,25]. In our study, it was observed that bacterial contaminants were tightly attached to a single colony of T. suecica due to large cell size and scaly cell wall.…”

Section: Discussionmentioning

confidence: 63%

“…Sonication produced shear forces that acted on the surface of algal cells to remove tightly attached bacterial contaminants [3]. The use of sonication treatment by ultrasonicator operated at 90 K cycles.s -1 for 5-20 min for purification of microalgae has also been reported by Brown and Bischoff [27] and Shirai [28].…”

Section: Discussionmentioning

confidence: 99%

“…Thus, for the bacterial contaminant which was sensitive (susceptible) to the particular antibiotic, a zone of inhibition around the disc impregnated with the antibiotic was appeared. Chemical method normally used antibiotics, singly or in combination, to kill or inhibit the growth of tenaciously attached contaminants [3]. The lethality of the antibiotic treatment is an intensity-time relationship.…”

Section: Antibiotic Treatmentmentioning

confidence: 99%

“…Ultrasonic water bath (Bransonic 2510E-MT, USA) was used at low sonication intensity (90 K cycles.s -1 ) as the step to physically separate contaminants from algal units [3]. In this technique, the test tube containing the algal unit was placed in ultrasonic water bath at 30 o C for various exposure times (1 to 30 min).…”

Section: Sonicationmentioning

confidence: 99%

“…Through algal biotechnology, various high-value compounds could be produced and isolated from numerous phototrophic and heterotrophic microalgae cultures [2]. Preparation of microalgae axenic culture is needed to obtain a viable culture of a single species, free of other species or "contaminants" [3]. The axenic culture of microalgae is required in large scale cultivation for production of various valuable products.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Improved Protocol for the Preparation of Axenic Culture and Adaptation to Heterotrophic Cultivation

Azma¹,

Mohamad²,

Rahim³

et al. 2010

TOBIOTJ

View full text Add to dashboard Cite

Abstract:The effectiveness of various physical and chemical methods for the removal of contaminants from the microalgae, Tetraselmis suecica, culture was investigated. The information obtained was used as the basis for the development of improved protocol for the preparation of axenic culture to be adapted to heterotrophic cultivation. Repeated centrifugation and rinsing effectively removed the free bacterial contaminants from the microalgae culture while sonication helped to loosen up the tightly attached bacterial contaminants on the microalgae cells. Removal of bacterial spores was accomplished using a mixture of two antibiotics, 5 mg/mL vancomycine and 10 mg/mL neomycine. Walne medium formulation with natural seawater was preferred for the enhancement of growth of T. suecica. Adaptation of growth from photoautotrophic to heterotrophic conditions was achieved by the repeated cultivation of photoautotrophic culture with sequential reduction in illumination time, and finally the culture was inoculated into the medium containing 10 g/L glucose, incubated in total darkness to obtain heterotrophic cells. Changes in the morphology and composition of T. suecica cells during the adaptation from photoautotrophic to heterotrophic condition, as examined under Transmission Electron Microscope, were also reported.

show abstract

Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Antibiotic Treatmentmentioning

confidence: 99%

Section: Sonicationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Improved Protocol for the Preparation of Axenic Culture and Adaptation to Heterotrophic Cultivation

Azma¹,

Mohamad²,

Rahim³

et al. 2010

TOBIOTJ

View full text Add to dashboard Cite

show abstract

Phycology

Borowitzka¹

2012

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

Phycology is the study of algae. However, just what algae are is difficult to define, because they belong to many different and unrelated taxonomic groups and include both prokaryotic and eukaryotic representatives. Broadly speaking, the algae comprise all, mainly aquatic, plants and plant‐like chlorophyll a ‐containing organisms that can use light energy to fix carbon from atmospheric carbon dioxide (CO 2 ) and evolve oxygen, but which are not specialised land plants like mosses, ferns, coniferous trees and flowering plants. This is a negative definition, but it serves its purpose. Besides being a principal source of oxygen, algae are important primary producers in many, mainly aquatic, habitats and are the source of a number of important commercial products and molecules. Key Concepts: The algae are a very diverse group of plants and plant‐like organisms but which are not specialised land plants like mosses, ferns, coniferous trees and flowering plants. The algae include both prokaryotic and eukaryotic organisms. Algae are key providers of oxygen on earth and are the basis of aquatic food webs. Algae have a wide range of commercial applications. Endosymbiosis has played an important part in the evolution of the algae.

show abstract

Phycology

Borowitzka¹

2020

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

Phycology is the study of algae. However, just what algae are is difficult to define, because they belong to many different and diverse taxonomic groups and include both prokaryotic and eukaryotic representatives. Broadly speaking, the algae comprise all, mainly aquatic, plants and plant‐like chlorophyll a ‐containing organisms that can use light energy to fix carbon from atmospheric carbon dioxide (CO 2 ) and evolve oxygen, but which are not specialised land plants like mosses, ferns, coniferous trees and flowering plants. This is a negative definition, but it serves its purpose. Besides being a principal source of oxygen, algae are important primary producers in many, mainly aquatic, habitats. The prokaryotic Cyanobacteria are important as nitrogen fixers, converting N 2 to organic nitrogen. The algae are the source of a number of important commercial products and molecules and are also used in wastewater treatment, as animal feed and as fertilisers. Key Concepts Phycology is the study of algae. The algae are a very diverse group of plants and plant‐like organisms, but which are not specialised land plants like mosses, ferns, coniferous trees and flowering plants. The algae include both prokaryotic and eukaryotic organisms. Algae are key providers of oxygen on earth and are the basis of aquatic food webs. Algae have a wide range of commercial applications. Endosymbiosis has played an important part in the evolution of the algae.

show abstract

Traditional Microalgae Isolation Techniques

Cited by 386 publications

References 28 publications

Improved Protocol for the Preparation of Axenic Culture and Adaptation to Heterotrophic Cultivation

Improved Protocol for the Preparation of Axenic Culture and Adaptation to Heterotrophic Cultivation

Phycology

Phycology

Contact Info

Product

Resources

About