TRAF2 phosphorylation promotes NF-κB–dependent gene expression and inhibits oxidative stress-induced cell death

Abstract

TRAF2 regulates JNK and IKK activation in response to TNF-α stimulation. This study found that TNF-α and oxidative stress induce TRAF2 phosphorylation and that this phosphorylation inhibits apoptosis by promoting the prolonged phase of IKK activation while inhibiting the prolonged phase of JNK activation.

Biochemical effects of combined action of ?-irradiation and paclitaxel on anaplastic thyroid cancer cells

“…Phosphorylation of Bcl-2 protein leads to inhibition of its protective, antiapoptotic properties [20]. Phosphorylated Bcl-2 also may degrade in proteasomes [21], as evidenced by decreasing amounts of this protein in ATC cells exposed to Ptx [7]. One can assume that, together with inhibition of Bcl-2 function, proapoptotic proteins other than Bax such as Bad, Bak and particularly (considering the fact of caspase-8 activation), tBid could be involved in the initiation of apoptotic process in mitochondria under combined action of Ptx and IR.…”

miR-892b Silencing Activates NF-κB and Promotes Aggressiveness in Breast Cancer

“…As a major component of the NF-kB cascade, TRAF2 functions as an E3 ligase and catalyzes K63-linked polyubiquitination of RIP1 in response to TNFa stimulation. The polyubiquitin chain of RIP1 serves as a platform to recruit and activate both the TAK1/TAB2/TAB3 complex and the IKK complex (19). In the present study, miR-892b was suggested to suppress the expression of TRAF2, leading to inhibition of RIP1 polyubiquitination and inhibition of the IKK complex.…”

“…Because TRAF2-mediated K-63 polyubiquitination of RIP1 and TAK1/TAB2/TAB3 complex-mediated phosphorylation of IKK-b contribute to NF-kB activation (18)(19)(20), the effects of miR-892b on the ubiquitination status of RIP1 and phosphorylation level of IKK-b were examined. As shown in Fig.…”

RIP1 Cleavage in the Kinase Domain Regulates TRAIL-Induced NF-κB Activation and Lymphoma Survival

“…The conventional and realtime reverse transcription (RT)-PCR assays were carried out as described previously (20,22).…”