Traffic light type paper-based analytical device for intuitive and semi-quantitative naked-eye signal readout

Abstract

Due to the continuously aging world population, there is a rapidly growing need for self-diagnostic tools that can be used by untrained users at home. Microfluidic paper-based analytical devices (µPADs)...

High-Throughput Microfluidic Production of Bimetallic Nanoparticles on MXene Nanosheets and Application in Hydrogen Peroxide Detection

“…H 2 O 2 is a byproduct of many oxidase-based enzymatic reactions of biomarkers, such as glucose, sarcosine, lactate, uric acid, choline, cholesterol, and pyruvate. Therefore, the detection of H 2 O 2 was important to biosensing.…”

“…Amperometric Detection of Hydrogen Peroxide. H 2 O 2 is a byproduct of many oxidase-based enzymatic reactions of biomarkers, 47 such as glucose, sarcosine, lactate, uric acid, choline, cholesterol, and pyruvate. Therefore, the detection of H Table 1 shows a comparison of electrochemical performance with other modified electrodes of functionalized MXene for H 2 O 2 detection.…”

Basic evaluation of the CRISPR/Cas system stability for application to paper-based analytical devices

“…When it comes to drying reagents on PADs, the reagent liquid volumes involved are generally much lower compared to the case of pharmaceutical preparations, thus drying of enzymes on paper Please do not adjust margins Please do not adjust margins by spontaneous drying at room temperature is a possible alternative. 10,12 In addition to drying methods, storage temperature is also an essential factor affecting the stability of proteins in the solid state. 25 Based on these facts, the storage stability of the CRISPR-related reagents was compared using the following three drying methods in combination with storage at various temperatures (25,4, and −20 ˚C): spontaneous drying at room temperature, drying at 37 ˚C, and freeze-drying.…”

“…7,8 Furthermore, designing microfluidic channels on PADs enables simultaneous multiplex target detection, 9, 10 control of multi-step reactions, 10,11 and liquid flow manipulation. 12,13 Therefore, PADs are regarded as a promising fundamental platform to realize POCT. Since the first report of a nucleic acid detection method using a CRISPR/Cas system, 14,15 a large variety of CRISPR-based assays has been realized.…”

Continuous production of bimetallic nanoparticles on carbon nanotubes based on 3D-printed microfluidics

“…Since the H 2 O 2 is produced by the enzyme-catalyzed reaction between numerous biomarkers, such as sarcosine, glucose, pyruvate and lactate, and the corresponding oxidase-family enzymes. 34,46 In this work, the optimized MWCNTs/Pt-Pd were used for the non-enzymatic detection of H 2 O 2 . Fig.…”