Trafficking Pathways and Characterization of CD4 and CD8 Cells Recruited to the Skin of Humans Experimentally Infected with<i>Haemophilus ducreyi</i>

Humphreys, Tricia L.; Baldridge, Lee Ann; Billings, Steven D.; Campbell, James J.; Spinola, Stanley M.

doi:10.1128/iai.73.7.3896-3902.2005

Cited by 22 publications

(11 citation statements)

References 40 publications

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“…T EM express a high expression level of β1 and β2 integrin and molecule involved in lymphocyte homing into the skin -cutaneous lymphocyte antigen (CLA) and the chemokines receptors such as CCR1, CCR3, CCR5 [2]. The T memory cells homing to skin exhibit expression of the CCR4 and CCR10 [36,41]. Moreover memory T cells homing into gut exhibit expression of α4β7 integrin and CCR9 [31,36].…”

Section: Discussionmentioning

confidence: 99%

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Effector and memory CD4+ and CD8+ T cells in the chronic infection process.

Ojdana

Safiejko²,

Lipska³

et al. 2009

Folia Histochem Cytobiol.

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T cell memory in comparison with B cell memory is not well understood. This review focuses on CD8+ and CD4+ memory T cells. In this article we try to define memory cells and also present models of memory T cells formation. We would also like to delineate their differentiation into distinct subsets. Long-lived memory T cells consist in two main subsets: T CM and T EM . Recent studies have shown that not all cells considered to be memory cells differentiate into T CM and T EM , but a small proportion of theses cells exhibit naive cells phenotype. Memory T cells constitute a heterogeneous population of cells. In this study we lay stress on characteristic of main memory T cells subsets and their alleged participation in immune response upon reexposure to the Ag.

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Section: Discussionmentioning

confidence: 99%

“…They have the potential to produce cytokines rapidly and eliminate infected cells [19,40]. T EM CD8+ efficiently produce IFN-γ, TNF-α, IL-4, IL-5 and perforins [14,41].…”

Section: Within Cd8+ T Cells Similarly To Memory Cd4+ T Cells May Be mentioning

confidence: 99%

Effector and memory CD4+ and CD8+ T cells in the chronic infection process.

Ojdana

Safiejko²,

Lipska³

et al. 2009

Folia Histochem Cytobiol.

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“…The T-cell infiltrate consists predominantly of memory CD4 cells (31). In pustules obtained from subjects infected once, the ratio of myeloid dendritic cells (DC) to plasmacytoid DC is 2.8-fold that in peripheral blood (5a).…”

Section: In Experimentally Infected Human Volunteers the Cutaneous Imentioning

confidence: 99%

Dysregulated Immune Profiles for Skin and Dendritic Cells Are Associated with Increased Host Susceptibility toHaemophilus ducreyiInfection in Human Volunteers

Humphreys¹,

Li²,

et al. 2007

Infect Immun

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In experimentally infected human volunteers, the cutaneous immune response to Haemophilus ducreyi is orchestrated by serum, polymorphonuclear leukocytes, macrophages, T cells, and myeloid dendritic cells (DC). This response either leads to spontaneous resolution of infection or progresses to pustule formation, which is associated with the failure of phagocytes to ingest the organism and the presence of Th1 and regulatory T cells. In volunteers who are challenged twice, some subjects form at least one pustule twice (PP group), while others have all inoculated sites resolve twice (RR group). Here, we infected PP and RR subjects with H. ducreyi and used microarrays to profile gene expression in infected and wounded skin. The PP and RR groups shared a core response to H. ducreyi. Additional transcripts that signified effective immune function were differentially expressed in RR infected sites, while those that signified a hyperinflammatory, dysregulated response were differentially expressed in PP infected sites. To examine whether DC drove these responses, we profiled gene expression in H. ducreyi-infected and uninfected monocyte-derived DC. Both groups had a common response that was typical of a type 1 DC (DC1) response. RR DC exclusively expressed many additional transcripts indicative of DC1. PP DC exclusively expressed differentially regulated transcripts characteristic of DC1 and regulatory DC. The data suggest that DC from the PP and RR groups respond differentially to H. ducreyi. PP DC may promote a dysregulated T-cell response that contributes to phagocytic failure, while RR DC may promote a Th1 response that facilitates bacterial clearance.

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“…A blood sample was obtained from each subject on the day of biopsy, and peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Paque gradient centrifugation. Biopsy samples were minced and processed exactly as described previously (15), except that single cells were collected using a 70-m sieve. Biopsy cells and PBMC were incubated with fluorescein isothiocyanate (FITC)-conjugated antibodies to lineage cocktail 1 (CD3 clone SK7, CD14 clone MP9, CD16 clone 3G8, CD19 clone SJ25C1, CD20 clone L27, and CD56 clone NCAM16.2), peridinin chlorophyll protein-conjugated antibodies to HLA-DR (clone G46-6), allophycocyanin (APC)-conjugated antibodies to CD11c (clone S-HCL-3), and phycoerythrin (PE)-conjugated antibodies to CD123 (clone 9F5) (all obtained from BD Biosciences).…”

Section: Characterization Of DC From Biopsy Samples Of H Ducreyi-infmentioning

confidence: 99%