Numerous variables can affect performance before the competition. Nevertheless, it could be a subject of curiosity to find out whether warm-up strategy has a more positive effect on the power performance of kickboxers. The purpose of this study is to evaluate the effect of different specific warm-up protocols and time of day on optimum power performance in kickboxers. Ten volunteer males performed three different warm-up routines in random order. The warm-up protocols consisted of only 20 min for each group. The groups with low-intensity aerobic running (NSWU) and specific warm-ups (SWU-20m) performed 20-min protocols. The other group followed 10 minutes of jogging and 10 minutes of specific warm-up (SWU-10m). All groups followed the warm-up randomly at two different periods of the day (i.e. morning: 09:00-10:00 and evening: 16:00-17:00) on non-consecutive days. A statistically significant difference was found between the groups in terms of Mean Propulsion (W) (F=3.518, p2=0.036). While there was a statistically significant difference in Mean propulsive Power (W) between NSWU and SWU-10m (p3= 0.0007) or SWU-10m and SWU-20m (p3= 0.0106) groups, NSWU and SWU-20m (p3= 0.415). In conclusion, mean propulsion (W) performance showed diurnal variation, and performances of the kickboxers’ can be affected more positively in the evening hours, especially after SWU protocols.