Training‐Induced Variations in Haematological and Biochemical Variables in Adolescent Athletes of Arab Origin Throughout an Entire Athletic Season

Varamenti, Evdokia; Николовски, Зоран; El-Gingo, Mohamed; Jamurtas, Athanasios Z.; Cardinale, Marco

doi:10.1515/hukin-2017-0187

Cited by 9 publications

(18 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Area under the curve with high dose anserine (ANS-HD), low dose anserine (ANS-LD), and placebo (PLA) treatments of the following blood biomarkers: Oxidative stress; superoxide dismutase (SOD), catalase (CAT), glutathione disulfide (GSSG), glutathione (GSH), GSH/GSSG ratio, and thiobarbituric acid active reactive substances (TBARS); cell damage; glutamic-oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), creatine kinase myocardial band (CKMB), and myoglobin ( Anserine's potential for augmenting cell damage repair adaptations may have reflected a preserved WBC ( Figure 5). However, RBC levels showed a small but significant increase in MCV (p < 0.01) and a decrease (~1%) in MCHC (p = 0.001) in ANS-HD compared with ANS-LD and PLA ( Figure 6), which is close to previously reported decreased MCHC following either intense exercise training (~1%-3%) [15] or a high intensity exercise training combined with a vitamin D supplement (~0.7%) [26]. Senescent RBCs are particularly prone to exercise-induced intravascular haemolysis and an associated…”

supporting

confidence: 90%

“…High intensity exercise produces ultrastructural muscular disruption, impaired excitation-contraction coupling, preferential fibre type damage, and impaired muscle metabolism, which in turn causes delayed onset of muscle soreness, swelling of the affected limb, decreased range of motion, and impaired muscle force producing capacity [12]. Alterations in cell damage biomarkers such as glutamic-oxaloacetic transaminase (GOT), glutamate pyruvate transaminase (GPT), creatine kinase isoenzyme (CKMB) and myoglobin, and associated adverse haematological responses of red blood cells (RBCs) and white blood cells (WBCs) have all been previously reported following single or repeated bouts of intense exercise [13][14][15]. For example, CKMB and myoglobin are known muscle damage biomarkers, and their levels increase several-fold immediately after exhaustive endurance exercise and can take up to 48 h to be restored close to its baseline levels, depending on the severity of exercise or the endurance of sporting event [14].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Anserine Reverses Exercise-Induced Oxidative Stress and Preserves Cellular Homeostasis in Healthy Men

et al. 2020

View full text Add to dashboard Cite

The study tested whether anserine (beta-alanyl-3-methyl-l-histidine), the active ingredient of chicken essence affects exercise-induced oxidative stress, cell integrity, and haematology biomarkers. In a randomized placebo-controlled repeated-measures design, ten healthy men ingested anserine in either a low dose (ANS-LD) 15 mg·kg−1·bw−1, high dose (ANS-HD) 30 mg·kg−1·bw−1, or placebo (PLA), following an exercise challenge (time to exhaustion), on three separate occasions. Anserine supplementation increased superoxide dismutase (SOD) by 50% (p < 0.001, effect size d = 0.8 for both ANS-LD and ANS-HD), and preserved catalase (CAT) activity suggesting an improved antioxidant activity. However, both ANS-LD and ANS-HD elevated glutathione disulfide (GSSG), (both p < 0.001, main treatment effect), and consequently lowered the glutathione to glutathione disulfide (GSH/GSSG) ratio compared with PLA (p < 0.01, main treatment effect), without significant effects on thiobarbituric acid active reactive substances (TBARS). Exercise-induced cell damage biomarkers of glutamic-oxaloacetic transaminase (GOT) and myoglobin were unaffected by anserine. There were slight but significant elevations in glutamate pyruvate transaminase (GPT) and creatine kinase isoenzyme (CKMB), especially in ANS-HD (p < 0.05) compared with ANS-LD or PLA. Haematological biomarkers were largely unaffected by anserine, its dose, and without interaction with post exercise time-course. However, compared with ANS-LD and PLA, ANS-HD increased the mean cell volume (MCV), and decreased the mean corpuscular haemoglobin concentration (MCHC) (p < 0.001). Anserine preserves cellular homoeostasis through enhanced antioxidant activity and protects cell integrity in healthy men, which is important for chronic disease prevention. However, anserine temporal elevated exercise-induced cell-damage, together with enhanced antioxidant activity and haematological responses suggest an augmented exercise-induced adaptative response and recovery.

show abstract

supporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Anserine Reverses Exercise-Induced Oxidative Stress and Preserves Cellular Homeostasis in Healthy Men

et al. 2020

View full text Add to dashboard Cite

show abstract

“…This could be due to plasma volume variations induced by effort in competition vs. out of competition (Morkeberg et al, 2009 ), or strength and endurance training periods (Collins et al, 1986 ; Imelik and Mustimets, 1992 ; Sawka et al, 2000 ). Little is known about any direct influence of training load variation (i.e., calculated during training and when power data in competitions are available) on hematological variables (Guglielmini et al, 1989 ; Varamenti et al, 2018 ). There are no studies investigating the influence of training load (including competitions) over a prolonged period on the ABP variables in elite cyclists.…”

Section: Introductionmentioning

confidence: 99%

The Influence of Training Load on Hematological Athlete Biological Passport Variables in Elite Cyclists

Astolfi

Roten

Kayser

et al. 2021

Front. Sports Act. Living

View full text Add to dashboard Cite

The hematological module of the Athlete Biological Passport (ABP) is used in elite sport for antidoping purposes. Its aim is to better target athletes for testing and to indirectly detect blood doping. The ABP allows to monitor hematological variations in athletes using selected primary blood biomarkers [hemoglobin concentration (Hb) and reticulocyte percentage (Ret%)] with an adaptive Bayesian model to set individual upper and lower limits. If values fall outside the individual limits, an athlete may be further targeted and ultimately sanctioned. Since (Hb) varies with plasma volume (PV) fluctuations, possibly caused by training load changes, we investigated the putative influence of acute and chronic training load changes on the ABP variables. Monthly blood samples were collected over one year in 10 male elite cyclists (25.6 ± 3.4 years, 181 ± 4 cm, 71.3 ± 4.9 kg, 6.7 ± 0.8 W.kg−1 5-min maximal power output) to calculate individual ABP profiles and monitor hematological variables. Total hemoglobin mass (Hbmass) and PV were additionally measured by carbon monoxide rebreathing. Acute and chronic training loads–respectively 5 and 42 days before sampling–were calculated considering duration and intensity (training stress score, TSSTM). (Hb) averaged 14.2 ± 0.0 (mean ± SD) g.dL−1 (range: 13.3–15.5 g·dl−1) over the study with significant changes over time (P = 0.004). Hbmass was 1030 ± 87 g (range: 842–1116 g) with no significant variations over time (P = 0.118), whereas PV was 4309 ± 350 mL (range: 3,688–4,751 mL) with a time-effect observed over the study time (P = 0.014). Higher acute–but not chronic—training loads were associated with significantly decreased (Hb) (P <0.001). Although individual hematological variations were observed, all ABP variables remained within the individually calculated limits. Our results support that acute training load variations significantly affect (Hb), likely due to short-term PV fluctuations, underlining the importance of considering training load when interpreting individual ABP variations for anti-doping purposes.

show abstract

“…The copyright holder for this preprint this version posted October 26, 2020. ; https://doi.org/10.1101/2020.10.22.20213413 doi: medRxiv preprint Athlete biological passport & training load Sawka et al, 2000). Little is known about any direct influence of training load variation on hematological variables (Guglielmini et al, 1989;Varamenti et al, 2018). There are no studies investigating the influence of training load over a prolonged period on the ABP variables in elite cyclists.…”

Section: Introductionmentioning

confidence: 99%

The influence of training load on hematological Athlete Biological Passport variables in elite cyclists

Astolfi

Fabienne

Kayser

et al. 2020

Preprint

View full text Add to dashboard Cite

The hematological module of the Athlete Biological Passport (ABP) is used in elite sport for antidoping purposes. Its aim is to better target athletes for testing and to indirectly detect blood doping. The ABP allows to monitor hematological variations in athletes using selected primary blood biomarkers (hemoglobin concentration ([Hb]) and reticulocyte percentage (Ret%)) with an adaptive Bayesian model to set individual upper and lower limits. If values fall without the individual limits, an athlete may be further targeted and ultimately sanctioned. Since Hb and Ret% vary with plasma volume (PV) fluctuations, possibly caused by training load changes, we investigated the putative influence of acute and chronic training load changes on the ABP variables. Monthly blood samples were collected over one year in 10 elite cyclists (Mean (SD): 25.6 (3.4) yrs, 181 (4) cm, 71.3 (4.9) kg, 6.7 (0.8) W/kg 5 min maximal power output) to calculate individual ABP profiles and monitor hematological variables. Total hemoglobin mass (Hbmass) and PV were additionally measured by carbon monoxide rebreathing. Acute and chronic training loads (respectively 5 and 42 days before sampling) were calculated considering duration and intensity (training stress score, TSSTM). [Hb] averaged 14.2 (0.0) (mean (SD)) g/dL (range: 13.3 to 15.5 g/dl) over the study with significant changes over time (P = 0.004). Hbmass was 1030 (87) g (range: 842 to 1116 g) with no significant variations over time (P = 0.118), whereas PV was 4309 (350) mL (range: 3688 to 4751 mL) with a time effect observed over the study time (P = 0.014). Higher acute (but not chronic) training loads were associated with significantly decreased Hb (P <0.001). Although individual hematological variations were observed, all ABP variables remained within the individually calculated limits. Our results support that acute training load variations significantly affect [Hb], likely due to short term PV fluctuations, underlining the importance of considering training load when interpreting individual ABP variations for anti-doping purposes.

show abstract

Training‐Induced Variations in Haematological and Biochemical Variables in Adolescent Athletes of Arab Origin Throughout an Entire Athletic Season

Cited by 9 publications

References 25 publications

Anserine Reverses Exercise-Induced Oxidative Stress and Preserves Cellular Homeostasis in Healthy Men

Anserine Reverses Exercise-Induced Oxidative Stress and Preserves Cellular Homeostasis in Healthy Men

The Influence of Training Load on Hematological Athlete Biological Passport Variables in Elite Cyclists

The influence of training load on hematological Athlete Biological Passport variables in elite cyclists

Contact Info

Product

Resources

About