2021
DOI: 10.1101/gr.271148.120
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Trajectory mapping of the early Drosophila germline reveals controls of zygotic activation and sex differentiation

Abstract: Germ cells in Drosophila melanogaster are specified maternally shortly after fertilization and are transcriptionally quiescent until their zygotic genome is activated to sustain further development. To understand the molecular basis of this process, we analyzed the progressing transcriptomes of early male and female germ cells at the single-cell level between germline specification and coalescence with somatic gonadal cells. Our data comprehensively cover zygotic activation in the germline genome, and analyses… Show more

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Cited by 6 publications
(12 citation statements)
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“…HP6 RNA was previously identified in the PGCs of embryos and germline stem cells of adult ovaries (Jevitt et al 2020;Rust et al 2020;Slaidina et al 2020;Li et al 2021;Slaidina et al 2021). In wildtype embryos, HP6 is expressed during gonad coalescence (Li et al 2021). Consistent with the complete deletion of the HP6 locus, we could not visualize HP6 RNA in the PGCs of HP6 CR2 null mutant embryos or the germline stem cells of HP6 CR1 /HP6 CR2 trans-heterozygous mutant ovaries (Fig.…”
Section: Generation Of An Hp6 Null Mutantssupporting
confidence: 68%
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“…HP6 RNA was previously identified in the PGCs of embryos and germline stem cells of adult ovaries (Jevitt et al 2020;Rust et al 2020;Slaidina et al 2020;Li et al 2021;Slaidina et al 2021). In wildtype embryos, HP6 is expressed during gonad coalescence (Li et al 2021). Consistent with the complete deletion of the HP6 locus, we could not visualize HP6 RNA in the PGCs of HP6 CR2 null mutant embryos or the germline stem cells of HP6 CR1 /HP6 CR2 trans-heterozygous mutant ovaries (Fig.…”
Section: Generation Of An Hp6 Null Mutantssupporting
confidence: 68%
“…1C&D). HP6 RNA was previously identified in the PGCs of embryos and germline stem cells of adult ovaries (Jevitt et al 2020;Rust et al 2020;Slaidina et al 2020;Li et al 2021;Slaidina et al 2021). In wildtype embryos, HP6 is expressed during gonad coalescence (Li et al 2021).…”
Section: Generation Of An Hp6 Null Mutantsmentioning
confidence: 99%
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“…Second, the question of whether dosage compensation even occurs in the germline has been controversial. Using whole-genome expression profiling, some researchers conclude that dosage compensation occurs in the germline [Gupta et al, 2006;Witt et al, 2021] while others conclude that it does not [Meiklejohn et al, 2011;Li et al, 2021;Ota et al, 2021]. Thus, the questions of whether dosage compensation exists in the germline and whether Sxl can regulate this process in an MSL-independent manner remain to be resolved.…”
Section: Dosage Compensation In the Germlinementioning
confidence: 99%