A hospital's physical pattern is a vital part of its contamination management measures to reduce the danger of spread of any communicable disease. Recent and rising communicable diseases challenges as higher public prospects and awareness of care connected problems, a lot of thought has got to incline to the layout of the hospital. In present study, airflow simulation of a multi-patients ICU room has been carried out using FLUENT version 15 CFD software. For simulation of airflow standard k-epsilon turbulence model used with high-quality unstructured mesh. Five different cases of multiple staff orientation have been studied to look at the infection between every patients and additionally for medical staff. In whole cases, inlet fresh airflow temperature (273 K) and airflow rate (0.2 m/s) are held constant. An average of 9.78 min time was taken by mobile contaminants to leave ICU room. The location of air inlet and outlet holds good air ventilation as particles coming out from the mouth of patient moves out of ICU in most of the instances and hospital staff orientations.