Trans-10, cis-12-conjugated linoleic acid attenuates tumor necrosis factor-α production by lipopolysaccharide-stimulated porcine peripheral blood mononuclear cells through induction of interleukin-10

Kim, Keun-Hwa; Kim, Dong‐In; Kim, Soohyun; Jung, Eui‐Man; Kang, Ji‐Houn; Jeung, Eui‐Bae; Yang, Mhan‐Pyo

doi:10.1016/j.cyto.2011.06.019

Cited by 11 publications

(11 citation statements)

References 29 publications

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“…Interleukin‐10, also known as cytokine synthesis inhibitory factor, is an anti‐inflammatory cytokine (3). IL‐10 inhibits the production of various pro‐inflammatory cytokines, including TNF‐α and IFN‐γ(3,14).…”

Section: Discussionmentioning

confidence: 99%

“…Cytokines are categorized as pro‐inflammatory or anti‐inflammatory and play a key role in the modulation of the intestinal mucosal immune system. Interleukin (IL)‐10 is an anti‐inflammatory cytokine mainly produced by monocyte/macrophages (1,2), and is also known to potently inhibit the capacity of innate immune cells to produce pro‐inflammatory mediators such as tumor necrosis factor (TNF)‐α(3). In the normal intestine, macrophages induced by macrophage colony‐stimulating factor (M‐CSF) produce large amounts of IL‐10 but no pro‐inflammatory cytokines such as IL‐12 and IL‐23, so they may act as anti‐inflammatory macrophages (4,5).…”

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confidence: 99%

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Production of Interleukin‐10 by Combining a Granulocyte and Monocyte Adsorption Carrier With Ulinastatin

et al. 2012

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Interleukin (IL)-10 is an anti-inflammatory cytokine mainly produced by monocytes and is essential for the induction of anti-inflammatory intestinal macrophages with macrophage colony-stimulating factor (M-CSF). Thus, IL-10- and M-CSF-rich conditions in colonic tissues seem to contribute to the improvement of pathological conditions in patients with inflammatory bowel diseases (IBD). We have already reported that ulinastatin, a serine protease inhibitor, increases M-CSF production during granulocyte/monocyte (GM) adsorption to cellulose acetate (CA) beads (carriers for Adacolumn therapy). However, the effects of ulinastatin on IL-10 production have not been clarified. The aim of the present study was to clarify the effects of ulinastatin on IL-10 production during GM adsorption by in vitro experiments. Peripheral blood was divided into four groups: (Control) no ulinastatin added, no contact with CA beads; (1) no ulinastatin added, contact with CA beads; (2) ulinastatin added, no contact with CA beads; and (3) ulinastatin added, contact with CA beads. After incubation, IL-10 in the plasma was measured. Compared with the level in the Control group, plasma IL-10 was significantly higher only in group 3, in which ulinastatin was added in the presence of CA beads, but did not increase in the absence of CA beads. These results suggest that ulinastatin synergistically increases IL-10 production with monocyte adsorption stimuli. By increasing not only M-CSF but also IL-10, a combination of ulinastatin and Adacolumn therapy may improve clinical efficacy for the treatment of IBD in terms of the induction of anti-inflammatory intestinal macrophages.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Production of Interleukin‐10 by Combining a Granulocyte and Monocyte Adsorption Carrier With Ulinastatin

et al. 2012

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“…But, IL-10 causes down-regulation of TNF-a production and exerts anti-inflammatory effect in LPSstimulated PBMCs [54]. Another report showed G-1, a G protein coupled estrogen receptor (GPER) agonist and Thalidomide can induce IL-10 expression directly acting on Th17 or hybrid T cell populations [55].…”

Section: Il-10 Inducing and Inhibiting Agentsmentioning

confidence: 98%

Role of interleukin-10 in breast cancer

Ullah

Changkija

Konwar

2011

Breast Cancer Res Treat

155

113

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Cytokines are low molecular weight regulatory proteins or glycoprotein that modulates the intensity and duration of immune response by stimulating or inhibiting the activation, proliferation, and/or differentiation of target cells. Different cytokines are known to have diverse role in breast cancer initiation and progression. Interleukin-10 (IL-10), a pleiotropic anti-inflammatory cytokine, induces immunosuppression and assists in escape from tumor immune surveillance. Like several other cytokines, IL-10 also can exert dual proliferative and inhibitory effect on breast tumor cells indicating a complex role of IL-10 in breast cancer initiation and progression. In this review, we tried to put together a comprehensive current view on significance of IL-10 in promotion, inhibition, and importance as prognosticator in breast cancer based on in vitro, in vivo, and clinical evidences. For literature collection, we conducted PubMed search with keywords "IL-10" and "breast cancer".

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“…The observations that IL-10 effectively reduced LPS-induced TNF-a production in me/me macrophages suggest that the IL-10/IL-10R signaling cascade remains intact in these cells. Because the role of IL-10 in regulating TNF-a production under in vitro as well as in vivo conditions is well established (53)(54)(55)(56)(57), it may be inferred that persistent TNF-a production in LPS-stimulated me/me macrophages can, at least in part, be attributed to decreased endogenous IL-10 production.…”

Section: Discussionmentioning

confidence: 99%

SHP-1–Pyk2–Src Protein Complex and p38 MAPK Pathways Independently Regulate IL-10 Production in Lipopolysaccharide-Stimulated Macrophages

Okenwa

Kumar

Rego

et al. 2013

The Journal of Immunology

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The role of tyrosine phosphatase Src homology region 2 domain-containing phosphatase (SHP)-1 in LPS-activated cytokine production and inflammation was investigated by determining TNF-α and IL-10 production in splenic macrophages employing SHP-1–null (me/me) mouse model. LPS-stimulated me/me splenic macrophages secreted significantly less IL-10 with concomitantly elevated levels of TNF-α compared with wild-type (WT) macrophages irrespective of LPS dose and duration of stimulation. IL-10 significantly inhibited LPS-induced TNF-α production in both me/me and WT macrophages. The critical requirement for SHP-1 in regulating LPS-induced IL-10 and TNF-α production was confirmed by interfering with SHP-1 expression in WT macrophages and by reconstituting me/me macrophages with the SHP-1 gene. To delineate the role of SHP-1 in positive regulation of LPS-induced IL-10 production, signaling proteins representing SHP-1 targets were examined. The results reveal that tyrosine kinases Src and proline-rich tyrosine kinase 2 (Pyk2) regulate SHP-1–dependent LPS-induced IL-10 production and infer that optimal LPS-induced IL-10 production requires an assembly of a protein complex consisting of SHP-1–Pyk2–Src proteins. Moreover, LPS-induced IL-10 production also requires activation of the p38 MAPK independent of SHP-1 function. Overall, to our knowledge our results show for the first time that SHP-1 acts as a positive regulator of LPS-induced IL-10 production in splenic macrophages through two distinct and independent SHP-1–Pyk2–Src and p38 MAPK pathways.

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Trans-10, cis-12-conjugated linoleic acid attenuates tumor necrosis factor-α production by lipopolysaccharide-stimulated porcine peripheral blood mononuclear cells through induction of interleukin-10

Cited by 11 publications

References 29 publications

Production of Interleukin‐10 by Combining a Granulocyte and Monocyte Adsorption Carrier With Ulinastatin

Production of Interleukin‐10 by Combining a Granulocyte and Monocyte Adsorption Carrier With Ulinastatin

Role of interleukin-10 in breast cancer

SHP-1–Pyk2–Src Protein Complex and p38 MAPK Pathways Independently Regulate IL-10 Production in Lipopolysaccharide-Stimulated Macrophages

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