trans-10,cis-12-Conjugated Linoleic Acid Reduces Leptin Secretion from 3T3-L1 Adipocytes

Kang, Kai; Pariza, Michael W.

doi:10.1006/bbrc.2001.5603

Cited by 65 publications

(35 citation statements)

References 20 publications

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“…Because leptin is secreted mainly by adipocytes, circulating levels correlate directly with body mass index in humans (Ahima and Flier, 2000), with a positive correlation between circulating leptin levels and adiposity also reported in pigs (Barb et al, 2001). As CLA is reported to reduce body fat content (Ostrowska et al, 2003), a reduction in plasma leptin levels would be expected in CLA-supplemented animals (Kang and Pariza, 2002) unless there is central or peripheral leptin resistance. Leptin, by binding to its receptors located in skeletal muscle and fat cells promotes energy dissipation and prevents fatty acid accumulation in these tissues (Ceddia, 2005).…”

Section: Discussionmentioning

confidence: 99%

Impact of dietary betaine and conjugated linoleic acid on insulin sensitivity, protein and fat metabolism of obese pigs

Fernández‐Fígares

Lachica

Martín

et al. 2012

Animal

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To determine possible mechanisms of action that might explain the nutrient partitioning effect of betaine and conjugated linoleic acid (CLA) in Iberian pigs and to address potential adverse effects, twenty gilts were restrictively fed from 20 to 50 kg BW Control, 0.5% betaine, 1% CLA or 0.5% betaine 1 1% CLA diets. Serum hormones and metabolites profile were determined at 30 kg BW and an oral glucose test was performed before slaughter. Pigs were slaughtered at 50 kg BW and livers were obtained for chemical and histological analysis. Decreased serum urea in pigs fed betaine and betaine 1 CLA diets (11%; P 5 0.0001) indicated a more efficient N utilization. The increase in serum triacylglycerol (58% and 28%, respectively; P 5 0.0098) indicated that CLA and betaine 1 CLA could have reduced adipose tissue triacylglycerol synthesis from preformed fatty acids. Serum glucose, lowdensity lipoprotein (LDL) cholesterol and non-esterified fatty acids were unaffected. CLA and betaine 1 CLA altered serum lipids profile, although liver of pigs fed CLA diet presented no histopathological changes and triglyceride content was not different from Control pigs. Compared with controls, serum growth hormone decreased (20% to 23%; P 5 0.0209) for all treatments. Although serum insulin increased in CLA, and especially in betaine 1 CLA pigs (28% and 83%; P 5 0.0001), indices of insulin resistance were unaffected. In conclusion, CLA, and especially betaine 1 CLA, induced changes in biochemical parameters and hormones that may partially explain a nutrient partitioning effect in young pigs. Nevertheless, they exhibited weak, although detrimental, effects on blood lipids. Moreover, although livers were chemically and histologically normal, pigs fed CLA diet challenged with a glucose load had higher serum glucose than controls.

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Section: Discussionmentioning

confidence: 99%

Impact of dietary betaine and conjugated linoleic acid on insulin sensitivity, protein and fat metabolism of obese pigs

Fernández‐Fígares

Lachica

Martín

et al. 2012

Animal

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“…Oleic acid increased differentiation over controls in3T3-L1 cells (Guo et al, 2000), and human preadipocytes . Linoleic acid has been reported to increase differentiation over control in human preadipocytes (Brown and McIntosh, 2003;Hutley et al, 2003) and 3T3-L1 adipocytes (Kang and Pariza, 2001;Kang et al, 2003). In each of these studies, cells were induced to differentiate with a hormone 'cocktail', and the resulting cultures were continuously maintained in media containing either no serum, but including a hormone 'cocktail' (Hutley et al, 2003), or FCS plus or minus FA and apparently high (a) Day 4, overall treatment effect, GLUT-4 was increased v. control, (P , 0.001).…”

Section: Discussionmentioning

confidence: 99%

“…concentrations of insulin for a further 2 to 12 days (Kang and Pariza, 2001;Hutley et al, 2003;Kang et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Regulation of lipid accumulation in 3T3-L1 cells: insulin-independent and combined effects of fatty acids and insulin

Kokta

Strat

Papasani

et al. 2008

Animal

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The insulin-independent and combined effects of fatty acids (FA; linoleic and oleic acids) and insulin in modulating lipid accumulation and adipogenesis in 3T3-L1 cells was investigated using a novel protocol avoiding the effects of a complex hormone 'induction' mixture. 3T3-L1 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) plus serum (control) or in DMEM plus either 0.3 mmol/l linoleic or oleic acids with 0.3 mmol/l FA-free bovine serum albumin in the presence or absence of insulin. Cells were cultured for 4 to 8 days and cell number, lipid accumulation, peroxisome proliferator-activated receptor-gamma (PPAR-g) and glucose transporter 4 (GLUT-4) protein expression were determined. Cell number appeared to be decreased in comparison with control cultures. In both oleic acid and linoleic acid-treated cells, notably in the absence (and presence) of insulin, oil-red O stain-positive cells showed abundant lipid. The percentage of cells showing lipid accumulation was greater in FA-treated cultures compared with control cells grown in DMEM plus serum (P , 0.001). Treatment with both linoleic and oleic acid-containing media evoked higher levels of PPAR-g than observed in control cultures (P , 0.05). GLUT-4 protein also increased in response to treatment with both linoleic and oleic acid-containing media (P , 0.001). Lipid accumulation in 3T3-L1 cells occurs in response to either oleic or linoleic acids independently of the presence of insulin. Both PPAR-g and GLUT-4 protein expression were stimulated. Both proteins are considered markers of adipogenesis, and these observations suggest that these cells had entered the physiological state broadly accepted as differentiated. Furthermore, 3T3-L1 cells can be induced to accumulate lipid in a serum-free medium supplemented with FA, without the use of induction protocols using complex hormone mixtures. We have demonstrated a novel model for the study of lipid accumulation that will improve the understanding of adipogenesis in adipocyte lineage cells.Keywords: adipogenesis, fatty acids, GLUT-4, insulin, PPAR-g IntroductionProgression to lipid storage in adipocytes is characterised by two distinct phases. In the first step of hyperplastic expansion, progenitor cells are thought to become committed to the adipocyte lineage, after which they cannot revert back to a less differentiated 'stem-like' cell (Thompson et al., 1998;Boone et al., 2000). Once committed, adipoblasts undergo an exponential replication phase that terminates and the cell cycle arrests at gap 1 (G1). Early markers of differentiation, such as lipoprotein lipase (LPL) are then expressed, and these cells, known as preadipocytes, may then undergo proliferation (Boone et al., 2000). After preadipocytes stop proliferating, late markers of differentiation, such as glycerol-3-phosphate dehydrogenase (GPDH) and fatty acid synthetase (FAS) are detected. Cells then begin lipid accumulation in the cytosol at which time cells are termed adipocytes (Boone et al., 2000).Much of our understanding of these proc...

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“…Therefore, CLA might also directly affect leptin production. For example, incubation of 3T3-L1 adipocytes with trans-10, cis-12-CLA (t10c12 isomer: the CLA isomer suggested to be most effective at reducing fat mass in animals) inhibited leptin secretion and expression (Kang and Pariza, 2001). However, another more recent study found that the t10c12 isomer increased leptin gene expression in cultures of stromal vascular cells (SV cells) containing newly differentiated human adipocytes (Brown et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Conjugated linoleic acid inhibits glucose metabolism, leptin and adiponectin secretion in primary cultured rat adipocytes

Pérez-Matute

Marti

Martínez

et al. 2007

Molecular and Cellular Endocrinology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.A c c e p t e d M a n u s c r i p t

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trans-10,cis-12-Conjugated Linoleic Acid Reduces Leptin Secretion from 3T3-L1 Adipocytes

Cited by 65 publications

References 20 publications

Impact of dietary betaine and conjugated linoleic acid on insulin sensitivity, protein and fat metabolism of obese pigs

Impact of dietary betaine and conjugated linoleic acid on insulin sensitivity, protein and fat metabolism of obese pigs

Regulation of lipid accumulation in 3T3-L1 cells: insulin-independent and combined effects of fatty acids and insulin

Conjugated linoleic acid inhibits glucose metabolism, leptin and adiponectin secretion in primary cultured rat adipocytes

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