Trans-activation of TRPV1 by D1R in mouse dorsal root ganglion neurons

Lee, Dong Woo; Cho, Pyung Sun; Lee, Han Kyu; Lee, Sang Hoon; Jung, Sung Jun; Oh, Seog Bae

doi:10.1016/j.bbrc.2015.08.096

Cited by 7 publications

(5 citation statements)

References 35 publications

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“…Together with D 1 and D 5 immunoreactivities in TG neurons (Supplementary Fig. S2d,e ), the acute behavioural and cellular responses by dopamine receptor subtype specific agonists suggest that almost all subtypes are expressed in peripheral sensory nerves of mice, consistent with many early studies in TG and dorsal root ganglion neurons 32 – 37 . Pigment B6 sensory neurons demonstrated lower expression of D 1 , (but not D 5 ) compared with albino-B6 neurons, as measured by immunofluorescence (Supplementary Fig.…”

Section: Resultssupporting

confidence: 83%

“…We infer from these results that dopamine-induced Ca 2+ response is predominantly mediated by D 1 -like receptors. SKF38393-induces a Ca 2+ response in cultured B6 dorsal root ganglion neurons that are mediated by Ca 2+ influx from the extracellular space via TRPV1, as opposed to intracellular Ca 2+ stores 32 . The excitation effect of SKF38393 on cultured sensory neurons is consistent with the SKF38393-mediated heat hypersensitivity in the acute phase (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 95%

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Cutaneous pigmentation modulates skin sensitivity via tyrosinase-dependent dopaminergic signalling

Ono

Viet

et al. 2017

Sci Rep

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We propose a new mechanism of sensory modulation through cutaneous dopaminergic signalling. We hypothesize that dopaminergic signalling contributes to differential cutaneous sensitivity in darker versus lighter pigmented humans and mouse strains. We show that thermal and mechanical cutaneous sensitivity is pigmentation dependent. Meta-analyses in humans and mice, along with our own mouse behavioural studies, reveal higher thermal sensitivity in pigmented skin relative to less-pigmented or albino skin. We show that dopamine from melanocytes activates the D1-like dopamine receptor on primary sensory neurons. Dopaminergic activation increases expression of the heat-sensitive TRPV1 ion channel and reduces expression of the mechanically-sensitive Piezo2 channel; thermal threshold is lower and mechanical threshold is higher in pigmented skin.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 95%

Cutaneous pigmentation modulates skin sensitivity via tyrosinase-dependent dopaminergic signalling

Ono

Viet

et al. 2017

Sci Rep

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“…Previous studies have indicated that descending pain modulation of dopamine in the spinal cord is related to the dopaminergic nerve and D2‐like receptors (Taniguchi et al, ; Lee et al, ). What's more, studies have shown a close relationship between the dopamine system and transient receptor potential vanilloid 1, a transducer of noxious heat (Davis et al, ), in the ventral tegmental area where dopaminergic neurons project to the nucleus accumbens and prefrontal cortex (Marinelli et al, ).…”

Section: Discussionmentioning

confidence: 99%

Dopamine D3 receptor knockout mice exhibit abnormal nociception in a sex‐different manner

Liu

Xing

Chu

et al. 2016

J of Neuroscience Research

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Pain is a complex and subjective experience. Previous studies have shown that mice lacking the dopamine D3 receptor (D3RKO) exhibit hypoalgesia, indicating a role of the D3 receptor in modulation of nociception. Given that there are sex differences in pain perception, there may be differences in responses to nociceptive stimuli between male and female D3RKO mice. In the current study, we examined the role of the D3 receptor in modulating nociception in male and female D3RKO mice. Acute thermal pain was modeled by hot-plate test. This test was performed at different temperatures including 52°C, 55°C, and 58°C. The von Frey hair test was applied to evaluate mechanical pain. And persistent pain produced by peripheral tissue injury and inflammation was modeled by formalin test. In the hot-plate test, compared with wild-type (WT) mice, D3RKO mice generally exhibited longer latencies at each of the three temperatures. Specially, male D3RKO mice showed hypoalgesia compared with male WT mice when the temperature was 55°C, while for the female mice, there was a statistical difference between genotypes when the test condition was 52°C. In the von Frey hair test, both male and female D3RKO mice exhibited hypoalgesia. In the formalin test, the male D3RKO mice displayed a similar nociceptive behavior as their sex-matched WT littermates, whereas significantly depressed late-phase formalin-induced nociceptive behaviors were observed in the female mutants. These findings indicated that the D3 receptor affects nociceptive behaviors in a sex-specific manner and that its absence induces more analgesic behavior in the female knockout mice. © 2016 Wiley Periodicals, Inc.

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“…Most notably, activation of D1-like DA receptors in dorsal root ganglion (DRG) neurons induced Ca2 + influx via TRPV1 trans-activation. In addition, D1-like DA receptors and TRPV1 are co-expressed in mouse DRG neurons (Lee et al, 2015). However, the role of D1-like DA receptors and TRPV1 interaction in the NAc during cocaine-induced behaviors is unclear.…”

Section: Introductionmentioning

confidence: 99%

Transient receptor potential vanilloid 1 mediates cocaine reinstatement via the D1 dopamine receptor in the nucleus accumbens

You

Hong

et al. 2019

J Psychopharmacol

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Purpose: The transient receptor potential vanilloid 1 (TRPV1) is a nonselective cation channel that mediates synaptic modification in the nucleus accumbens (NAc). However, no study has yet examined the mechanism of TRPV1 in the NAc on cocaine reinstatement. We investigated the mechanism of TRPV1 in NAc on cocaine reinstatement using the conditioned place preference (CPP) test in mice. Methods: We examined the effect of capsazepine (5 mg/kg, a TRPV1 antagonist, administered intraperitoneally (i.p.)), capsaicin (0.3 mg/kg, a TRPV1 agonist, administered i.p.), and genetic deletion of TRPV1 on the reinstatement of cocaine-induced CPP (15 mg/kg, administered i.p.). The expression of TRPV1 and Ca2+/calmodulin-mediated kinase II (CaMKII) in the NAc were determined after cocaine reinstatement. Microinjection of SB366791 (0.2 ng, a selective TRPV1 antagonist) in the NAc was assessed on SKF-81297 (1 µg, D1-like dopamine (DA) receptor agonist) primed cocaine reinstatement. Results: Capsazepine suppressed and capsaicin potentiated cocaine CPP in the reinstatement phase. In addition, genetic deletion of TRPV1 inhibited cocaine-priming reinstatement. Cocaine reinstatement was mediated by increased TRPV1 expression in the NAc, which involves CaMKII. Microinjection of SB366791 in the NAc prevented the cocaine reinstatement evoked by microinjection of SKF-81297 in the NAc. Conclusions: These findings suggest that activation of TRPV1 mediates the stimulation of D1-like DA receptors and CaMKII in the NAc, resulting in the facilitation of cocaine reinstatement behaviors. Thus, our findings reveal a previously unknown TRPV1 mechanism in the reinstatement to drugs of abuse.

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Trans-activation of TRPV1 by D1R in mouse dorsal root ganglion neurons

Cited by 7 publications

References 35 publications

Cutaneous pigmentation modulates skin sensitivity via tyrosinase-dependent dopaminergic signalling

Cutaneous pigmentation modulates skin sensitivity via tyrosinase-dependent dopaminergic signalling

Dopamine D3 receptor knockout mice exhibit abnormal nociception in a sex‐different manner

Transient receptor potential vanilloid 1 mediates cocaine reinstatement via the D1 dopamine receptor in the nucleus accumbens

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