The mandate to label food products with the content of total trans fatty acids has led to an increase in demand for sensitive and accurate methodologies for the rapid quantitation of trans fats. Unfortunately, the latest official infrared (IR) spectroscopic method lacks the required sensitivity. A more sensitive IR procedure that requires the measurement of the height of the second derivative (2D) of the trans absorption band at 966 cm -1 was recently proposed; however, a reported inconsistency at low trans levels between GC (0% of total fat) and IR (1.2% of total fat) results for a fully hydrogenated vegetable oil could not be reconciled, and triggered further investigations. For the first time, we recognize and report the presence of weak interference bands (962-956 cm -1 ) attributed to saturated fats in the IR spectra of trans fats; these interference bands have an adverse impact on the sensitivity and accuracy of the IR determination at low trans levels (£0.5% of total fat). Therefore, weak spectral features observed at energies below the one expected for trans bands (966 cm -1 ) in test samples high in saturated fat (coconut oil and cocoa butter) must not be mistaken for trans bands. Tristearin TL Trilaurin TM Trimyristin TP Tripalmitin TA Triarachidin Since trans fat labeling requirements became mandatory in the US, Canada and many other countries [1], there has been an urgent need for accurate analytical methodologies that would facilitate the verification of compliance with the various regulations. The determination of total trans fatty acids by IR has been a widely used procedure [2] that has been standardized [3-6]. Its importance stems from the fact that the C-H out-of-plane deformation band observed at 966 cm -1 is uniquely characteristic of isolated double bonds with trans configuration. These double bonds are found in trans-monoenes, and in methylene-interrupted and non-methylene-interrupted trans,trans-dienes, mono-trans-dienes, and mono-trans-trienes. All these different fatty acids exhibit that same absorption band at 966 cm -1 regardless of chain length or position of the isolated trans double bond. Thus measuring the intensity of the absorption of the trans band effectively adds up all the different fatty acids containing isolated M. M. Mossoba (&) Food and Drug Administration,
Abbreviations
IR Infrared HSBO Hydrogenated soybean oil TS