Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae.

Bundock, Paul; Dulk‐Ras, Amke den; Beijersbergen, Alice; Hooykaas, Paul J. J.

doi:10.1002/j.1460-2075.1995.tb07323.x

Cited by 581 publications

(430 citation statements)

References 55 publications

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“…The largest subfamily, the conjugation systems, are found in most species of Gram-negative and Grampositive bacteria. These systems mediate DNA transfer both within and between phylogenetically diverse species, and some systems even deliver DNA to fungi, plants and human cells 2,[9][10][11][12][13] . Conjugation is an important contributor to genome plasticity, and therefore bacterial fitness under changing environmental conditions, as encountered during infection of the human host.…”

Section: The T4s Familymentioning

confidence: 99%

The versatile bacterial type IV secretion systems

Cascales¹,

Christie²

2003

Nat Rev Microbiol

601

559

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Bacteria use type IV secretion systems for two fundamental objectives related to pathogenesisgenetic exchange and the delivery of effector molecules to eukaryotic target cells. Whereas gene acquisition is an important adaptive mechanism that enables pathogens to cope with a changing environment during invasion of the host, interactions between effector and host molecules can suppress defence mechanisms, facilitate intracellular growth and even induce the synthesis of nutrients that are beneficial to bacterial colonization. Rapid progress has been made towards defining the structures and functions of type IV secretion machines, identifying the effector molecules, and elucidating the mechanisms by which the translocated effectors subvert eukaryotic cellular processes during infection. The year 2003 marks the fiftieth anniversary of the first description of a TYPE IV SECRETION (T4S)SYSTEM: the CONJUGATION apparatus of the F plasmid 1 . This is a dynamic bacterial surface organelle, the activities of which are now known to include the contact-dependent delivery of DNA to bacterial recipients and the assembly and retraction of a conjugal PILUS 2 . In the past decade, reports describing systems that are ancestrally related to the F-transfer system and other conjugation machines have emerged. Instead of mediating DNA transfer between bacteria, these systems deliver DNA or protein substrates, known as effectors, to eukaryotic target cells during infection [3][4][5] . More recently, several new T4S systems have been described that are also ancestrally related to the conjugation machines, but these systems mediate the exchange of DNA with the extracellular milieu [6][7][8] . Collectively, this diversity of function in the face of a common ancestry makes the T4S machines attractive subjects for comparative studies that explore the dynamics of organelle assembly and action. Additionally, from a medical perspective, it is of enormous interest to develop a detailed understanding of how the inter-kingdom transfer of type IV effector molecules contributes to pathogenesis. This review will summarize the recent advances in our knowledge of T4S, NIH Public Access Author ManuscriptNat Rev Microbiol. Author manuscript; available in PMC 2013 December 27. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscriptwith an emphasis on machine structure and function, and the activities of effectors after translocation into the eukaryotic host. The T4S familyThis fascinatingly versatile translocation family can be classified into three subfamilies, each of which contributes in unique ways to pathogenesis ( Fig. 1; Table 1). The largest subfamily, the conjugation systems, are found in most species of Gram-negative and Grampositive bacteria. These systems mediate DNA transfer both within and between phylogenetically diverse species, and some systems even deliver DNA to fungi, plants and human cells 2,9-13 . Conjugation is an important contributor to genome plasticity, and therefore bacterial fitness under changing en...

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Section: The T4s Familymentioning

confidence: 99%

The versatile bacterial type IV secretion systems

Cascales¹,

Christie²

2003

Nat Rev Microbiol

601

559

View full text Add to dashboard Cite

show abstract

“…Agrobacterium has been used extensively to 315 transfer DNA to eukaryotic algae in the green algal lineage with subsequent chromosomal integration (29)(30)(31)(32). In contrast, to our knowledge, the only other example beyond our results of using A. tumefaciens to deliver DNA maintained as an episome in the recipient was with yeast (14). During conjugation from E. coli to diatoms, it remains unclear how the DNA-protein 320 complex is transported to the recipient nucleus once in the eukaryotic cell.…”

Section: Discussionmentioning

confidence: 91%

“…tumefaciens-mediated transfer of episomes to P. tricornutum We also tested whether plasmids could be delivered by A. tumefaciens to the P. tricornutum and if they were maintained as episomes after transfer. In plants, A. tumefaciens is primarily used as a vehicle for insertion of DNA into the native chromosomes, yet Bundock et al (14) observed that the majority of DNA transferred to yeast by A. tumefaciens was maintained 255 episomally when the 2µm replication origin was included on the shuttle plasmid. We created two vectors to test transfer and maintenance.…”

Section: Plasmid Construction and Conjugation Experiments: 100mentioning

confidence: 99%

“…Transfers from E. coli to yeast (Saccharomyces cerevisiae) have been successfully 50 accomplished with the F-plasmid (MOB F , MPF F ) and IncP-based RP4 systems (MOB P , MPF P ) (12,13). Transfers to yeast using the Agrobacterium tumefaciens Ti-plasmid (MOB Q , MPF T ) have been reported (14). A. tumefaciens is perhaps the most well-known mediator of interdomain DNA transfers, transferring pathogenesis-related genes to a wide variety of plants during the course of various plant diseases such as crown gall.…”

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confidence: 99%

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Additional conjugation systems for inter-domain plasmid transfer to the diatom Phaeodactylum tricornutum

Kang

Bielinski

Bolt

et al. 2017

Preprint

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Bacterial conjugation utilizes a type IV secretion system and a DNA transfer mechanism to deliver DNA from one cell to another. Conjugative partners are conventionally confined to the prokaryotic domain. In a prominent exception, Agrobacterium tumefaciens type IV secretion-mediated transfer of DNA to plant cells can result in subsequent chromosomal integration. Recently, we demonstrated interdomain conjugation from Escherichia coli to the diatom Phaeodactylum tricornutum with the subsequent maintenance of an episome at chromosomal copy numbers if it contains diatom centromeres or centromere-like elements. The genes involved in the conjugation process can be separated into those encoding the type IV secretion system, also called the mating pair formation (MPF) genes, and genes involved in DNA processing called the mobilization (MOB) genes. Various protein families compose each class of conjugation genes, including common MOB types F, P, and Q and MPF types F, P, and T. The conjugative transfer from E. coli to P. tricornutum was demonstrated with a vector expressing MOBP and MTFP. Here we show that the MOBPsystem can be deleted and complemented with a MOBQ system in E.coli-diatom conjugations with subsequent episomal maintenaince. Utilization of both MOBP and MOBQ systems results in substantially higher efficiencies in E. coli-diatom conjugation. Finally, we demonstrate conjugative gene transfer between P. tricornutum and A. tumefaciens expressing a MPFT, the first demonstration of this system in diatoms,resulting in episomal maintainance or chromosomal integration, depending on the ex-conjugant. The promiscuity of MOB and MTF systems permitting prokaryote to diatom conjugative DNA transfer suggest major environmental and evolutionary importance of this process. The increased efficiency of dual MOB systems immediately improves genetic engineering in diatoms and has interesting basic cellular biology implications.

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“…Such a tool is necessary to clarify the role of those different genes in the symbiosis (e.g., are new transport events switched on as a result of interactions between the symbiotic partners?). Recently, a transformation protocol for ectomycorrhizal fungi based on the capacity of Agrobacterium tumefaciens to transfer its T-DNA to fungal cells as it does to plant cells (Bundock et al 1995) was developed by Pardo et al (2002). This method represents an interesting alternative to the traditional protoplast-mediated transformation, as this protocol can be implemented on intact fungal hyphae, thus circumventing the need for making protoplasts, which is tedious, time-consuming, and restricted to a limited number of ectomycorrhizal fungal species.…”

Section: Introductionmentioning

confidence: 99%

Functional expression of the green fluorescent protein in the ectomycorrhizal model fungus Hebeloma cylindrosporum

et al. 2006

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Hebeloma cylindrosporum is a model fungus for mycorrhizal studies because of its fast growth rate, simple nutritional requirements, and completion of its life cycle in vitro, and because it is amenable to transformation. To advance cell biological research during establishment of symbiosis, a tool that would enable the direct visualisation of fusion proteins in the different symbiotic tissues [namely, the expression of reporter genes such as Green Fluorescent Protein (GFP)] was still a missing tool. In the present study, H. cylindrosporum was transformed using Agrobacterium carrying the binary plasmid pBGgHg containing the Escherichia coli hygromycin B phosphotransferase (hph) and the EGFP genes, both under the control of the Agaricus bisporus glyceraldehyde-3-phosphate dehydrogenase promoter. EGFP expression was successfully detected in transformants. The fluorescence was uniformly distributed in the hyphae, while no significant background signal was detected in control hyphae. The suitability of EGFP for reporter gene studies in Hebeloma cylindrosporum was demonstrated opening up new perspectives in the Hebeloma genetics.

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Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae.

Cited by 581 publications

References 55 publications

The versatile bacterial type IV secretion systems

The versatile bacterial type IV secretion systems

Additional conjugation systems for inter-domain plasmid transfer to the diatom Phaeodactylum tricornutum

Functional expression of the green fluorescent protein in the ectomycorrhizal model fungus Hebeloma cylindrosporum

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