Trans10, cis12 conjugated linoleic acid inhibits 3T3-L1 adipocyte adipogenesis by elevating β-catenin levels

Yeganeh, Azadeh; Taylor, Carla G.; Poole, Jenna; Tworek, Leslee; Zahradka, Peter

doi:10.1016/j.bbalip.2016.01.004

Cited by 19 publications

(13 citation statements)

References 37 publications

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“…Accumulating evidence has demonstrated that c9, t11-CLA and t10, c12-CLA, two major isomers of CLA, have excellent biomedical properties for potential use in anticancer applications and for improving immunity, preventing inflammation, reducing obesity by different pathways such as Wnt/beta-catenin pathway, hormone-mediated mitogenic pathway, PPARc, 5-lipoxygenase (5-LOX) pathway and NF-jB pathway [6][7][8][12][13][14][15]. Although there was tremendous potential for the application of CLA isomers, their source of human daily intake was too limited to reach the recommended dosage, 3 g/d, which would be required to observe health benefits in human subjects [16].…”

Section: Resultsmentioning

confidence: 99%

“…In all of the possible cis and trans combinations of CLAs, c9, t11-CLA and t10, c12-CLA have been implicated as the most valuable isomers with noteworthy biological activities such as anti-carcinogenic, anti-obese, anti-diabetic and anti-hypertensive activities [4]. For example, it has been reported that c9, t11-CLA could inhibit the proliferation of estrogen receptor positive breast cancer cells by hormonemediated mitogenic pathways, and t10, c12-CLA could ameliorate disorders of glucose and lipid metabolism through PPARc and some other signal pathways [5][6][7][8]. Therefore, how to elevate the production of c9, t11-CLA and t10, c12-CLA has become a hot spot.…”

Section: Introductionmentioning

confidence: 99%

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Bioconversion of conjugated linoleic acid by Lactobacillus plantarum CGMCC8198 supplemented with Acer truncatum bunge seeds oil

Chen

Liu

et al. 2017

Food Sci Biotechnol

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Conjugated linoleic acid (CLA) isomers, 9,11-CLA and 10,12-CLA, have been proved to exhibit excellent biomedical properties for potential use in anti-cancer applications and in reducing obesity. (), which is rich in unsaturated fatty acids, including oleic acid, linoleic acid, and nervonic acid, is a new resource for edible oil. In the present study, we developed a new method for producing two CLA isomers from -seed oil by fermentation using CGMCC8198 (8198), a novel probiotics strain. Polymerase chain reaction results showed that there was a conserved linoleate isomerase (LIase) gene in 8198, and its transcription could be induced by-seed oil. Analyses by gas chromatography-mass spectrometry showed that the concentration of 9,11-CLA and 10,12-CLA in -seed oil could be increased by about 9- and 2.25-fold, respectively, after being fermented by8198.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioconversion of conjugated linoleic acid by Lactobacillus plantarum CGMCC8198 supplemented with Acer truncatum bunge seeds oil

Chen

Liu

et al. 2017

Food Sci Biotechnol

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“…To account for our observations, t10,c12‐CLA must either inhibit the ability of c9,t11‐CLA and thiazolidinediones to bind to their mitochondrial targets, or antagonize the activity of PPARγ. While there is no publication describing the effect of t10,c12‐CLA in modulating mitochondrial metabolism, evidence is emerging for t10,c12‐CLA antagonism of activated PPARγ activity (Granlund et al, ; Yeganeh et al, ). Because c9,t11‐CLA and t10,c12‐CLA are equally efficient in activating PPARγ (Granlund et al, ), the outcome cannot be attributed to binding of CLA isomers to PPARγ per se , but alternative pathways activated by CLA (Clément et al, ).…”

Section: Discussionmentioning

confidence: 99%

Cis‐9, Trans‐11 Conjugated Linoleic Acid Reduces Phosphoenolpyruvate Carboxykinase Expression and Hepatic Glucose Production in HepG2 Cells

Chai

Alshagga

Pan

et al. 2019

Lipids

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Dysregulated hepatic gluconeogenesis is a hallmark of insulin resistance and type 2 diabetes mellitus (T2DM). Although existing drugs have been proven to improve gluconeogenesis, achieving this objective with functional food is of interest, especially using conjugated linoleic acid (CLA) found in dairy products. Both cis‐9, trans‐11 (c9,t11) and trans‐10, cis‐12 (t10,c12) isomers of CLA were tested in human (HepG2) and rat (H4IIE) hepatocytes for their potential effects on gluconeogenesis. The hepatocytes exposed for 24 h with 20 μM of c9,t11‐CLA had attenuated the gluconeogenesis in both HepG2 and H4IIE by 62.5% and 80.1%, respectively. In contrast, t10,c12‐CLA had no effect. Of note, in HepG2 cells, the exposure of c9,t11‐CLA decreased the transcription of gluconeogenic enzymes, cytosolic phosphoenolpyruvate carboxykinase (PCK1) by 87.7%, and glucose‐6‐phosphatase catalytic subunit (G6PC) by 38.0%, while t10,c12‐CLA increased the expression of G6PC, suggesting the isomer‐specific effects of CLA on hepatic glucose production. In HepG2, the peroxisome proliferator‐activated receptor (PPAR) agonist, rosiglitazone, reduced the glucose production by 72.9%. However, co‐administration of c9,t11‐CLA and rosiglitazone neither exacerbated nor attenuated the efficacy of rosiglitazone to inhibit glucose production; meanwhile, t10,c12‐CLA abrogated the efficacy of rosiglitazone. Paradoxically, PPARγ antagonist GW 9662 also led to 70.2% reduction of glucose production and near undetectable PCK1 expression by abrogating CLA actions. Together, while the precise mechanisms by which CLA isomers modulate hepatic gluconeogenesis directly or via PPAR warrant further investigation, our findings establish that c9,t11‐CLA suppresses gluconeogenesis by decreasing PEPCK on hepatocytes.

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“…Yeganeh et al showed that CLA-t10,c12 treatment increased the levels of β-catenin and its activity in 3T3-L1 adipocytes. β-catenin binds to PPARγ, and inhibits its activity to prevent the progression of adipogenesis [ 47 , 48 ]. These researches confirmed that CLA inhibited adipocyte adipogenesis via Wnt/β-catenin signalling.…”

Section: Discussionmentioning

confidence: 99%

Effects of Conjugated Linoleic Acid Supplementation on the Expression Profile of miRNAs in Porcine Adipose Tissue

Wang

Liu

et al. 2017

Genes

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Conjugated linoleic acids (CLAs) play a major role in adipocyte differentiation and lipid metabolism in animals. MicroRNAs (miRNAs) appear to be involved in many biological processes in adipose tissue. However, the specific influence on miRNAs by CLA supplementation in porcine adipose tissue remains unclear. Thus, we continuously added 1.5% CLA to the pig diet from the embryo stage to the finishing period and conducted a high-throughput sequencing approach to analyse the changes in adipose tissue miRNAs. We identified 283 known porcine miRNAs, and 14 miRNAs were differentially expressed in response to CLA treatment. A Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the targets of the 14 differentially expressed miRNAs were involved in the Wnt signalling pathway. The CLA treatment downregulated the gene expression of PPARγ, C/EBPα, FAS, and FATP1 in both subcutaneous and abdominal fat tissues; the analysis showed that ssc-miR-21 expression was significantly correlated with PPARγ expression (p < 0.05), and speculated that ssc-miR-21 might influence adipogenesis through PPARγ. In conclusion, our study analysed the miRNA profiles in porcine adipose tissues by CLA treatment, and demonstrated that miRNAs are important regulators of fat lipogenesis. This study provides valuable information for the molecular regulatory mechanism of CLA on adipose tissue.

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Trans10, cis12 conjugated linoleic acid inhibits 3T3-L1 adipocyte adipogenesis by elevating β-catenin levels

Cited by 19 publications

References 37 publications

Bioconversion of conjugated linoleic acid by Lactobacillus plantarum CGMCC8198 supplemented with Acer truncatum bunge seeds oil

Bioconversion of conjugated linoleic acid by Lactobacillus plantarum CGMCC8198 supplemented with Acer truncatum bunge seeds oil

Cis‐9, Trans‐11 Conjugated Linoleic Acid Reduces Phosphoenolpyruvate Carboxykinase Expression and Hepatic Glucose Production in HepG2 Cells

Effects of Conjugated Linoleic Acid Supplementation on the Expression Profile of miRNAs in Porcine Adipose Tissue

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