Transactivation of SOX5 by Brachyury promotes breast cancer bone metastasis

Chen, Ming; Zou, Shitao; He, Chao; Zhou, Jundong; Li, Suoyuan; Shen, Minghong; Cheng, Rulei; Wang, Donglai; Zou, Tianming; Yan, Xinmin; Huang, Ying; Shen, Jun

doi:10.1093/carcin/bgz142

Cited by 21 publications

(34 citation statements)

References 46 publications

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“…Silencing of BRACHYURY in breast MDA-MB-436 cells statistically significantly reduced the ability to invade the ECM and form mammospheres in primary and secondary cultures (9). Our study (60) demonstrated that BRACHYURY promoted breast cancer cell invasion, migration, adhesion, and colonization in bone microenvironment in vitro, and BRACHYURY knockdown in MDA-MB-231 cells decreased the colonization and survival capability in bone tissue in vivo. BRACHYURY-high breast tumor cells were more resistant to the cytotoxic effects of docetaxel in vitro (9).…”

Section: Breast Carcinomasupporting

confidence: 51%

“…BRACHYURY/BRACHYURY expression has been reported to positively associate with the invasive and metastatic capability of breast carcinoma cells in vitro and with the risk of recurrence and distal metastasis in breast patients (9,60). Different studies have demonstrated the potential of BRACHYURY as a target for the treatment of breast carcinoma using cancer vaccines or immunotherapy approaches (61,62).…”

Section: Breast Carcinomamentioning

confidence: 99%

“…BRACHYURY/BRACHYURY is obviously highly expressed at the mRNA and protein levels in breast cancer tissues and cell lines compared to negativity in normal breast cancer tissues and cells (9,60,(63)(64)(65)(66). Hormone receptor status of breast cancer is an important and recognized prognostic factor and can reflect different stages (67), including estrogen receptor (ER) and progesterone receptor (PR).…”

Section: Breast Carcinomamentioning

confidence: 99%

“…Various studies have reported divergent effects of BRACHYURY on cell proliferation. Some demonstrated BRACHYURY promoted tumor cell growth and proliferation in vitro, including chordoma, prostate cancer, colorectal cancer, adenoid cystic carcinoma, and breast carcinoma cells (14,31,40,42,49,50,60,75,81). Whereas, others showed that BRACHYURY inhibited tumor cell growth and proliferation, including breast carcinoma, lung, and colorectal cells (9,10,82).…”

Section: The Effect Of Brachyury On Tumor Cell Proliferation and Cellmentioning

confidence: 99%

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The Roles of Embryonic Transcription Factor BRACHYURY in Tumorigenesis and Progression

Chen

Zhang

et al. 2020

Front. Oncol.

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Transcription factor brachyury, with a DNA-binding T-domain, regulates posterior mesoderm formation and notochord development through binding with highly conserved palindromic consensus sequence in a variety of organisms. The absence of brachyury expression in majority of adult normal tissues and exclusive tumor-specific expression provides the potential to be developed into a novel and promising diagnostic and therapeutic target in cancer. As a sensitive and specific marker in the diagnosis of chordoma, brachyury protein has been verified to involve in the process of carcinogenesis and progression of chordoma and several epithelial carcinomas in various studies, but the mechanism by which brachyury promotes tumor cells migrate, invade and metastasis still remains less clear. To this end, we attempt to summarize the literature on the upstream regulatory pathway of brachyury transcription and downstream controlling network by brachyury activation, all of which involve in both the embryonic development and tumor progression. We present the respective correlation of brachyury expression with tumor progression, distant metastasis, survival rate and prognosis in several types of tumor samples (including chordoma, lung cancer, breast carcinoma, and prostate cancer), and various brachyury gain-of-function and loss-of-function experiments are summarized to explore its specific role in respective tumor cell line in vitro. In addition, we also discuss another two programs relating to brachyury function: epithelial-to-mesenchymal transition (EMT) and cell cycle control, both of which implicate in the regulation of brachyury on biological behavior of tumor cells. This review will provide an overview of the function of master transcriptional factor brachyury, compare the similarities and differences of its role between embryonic development and carcinogenesis, and list the evidence on which brachyury-target therapies have the potential to help control advanced cancer populations.

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Section: Breast Carcinomasupporting

confidence: 51%

Section: Breast Carcinomamentioning

confidence: 99%

Section: Breast Carcinomamentioning

confidence: 99%

Section: The Effect Of Brachyury On Tumor Cell Proliferation and Cellmentioning

confidence: 99%

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The Roles of Embryonic Transcription Factor BRACHYURY in Tumorigenesis and Progression

Chen

Zhang

et al. 2020

Front. Oncol.

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“…The human brachyury protein (Bry), the transcriptional factor regulating posterior mesoderm formation and notochord differentiation, has been reported as a specific and sensitive marker ( 2 – 5 ) and a master regulator of the oncogenic transcriptional network of chordoma ( 6 ). Further studies demonstrated up-regulation of brachyury gene occurs in various human tumors of epithelial origin, including lung, breast, colorectal, prostate cancer and others, but not in the majority of normal adult tissues ( 7 – 9 ). In primary lung carcinoma samples, brachyury mRNA expression was identified as a significant predictor in 5 year disease free survival and overall survival rate ( 10 ) and positively correlated with tumor stage and poor prognosis ( 8 , 10 , 11 ).…”

Section: Introductionmentioning

confidence: 99%

The Role of Transcriptional Factor Brachyury on Cell Cycle Regulation in Non-small Cell Lung Cancer

Chen

et al. 2020

Front. Oncol.

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Lung cancer is the leading cause of cancer-related death, and non-small cell lung cancer (NSCLC) accounts for almost 80-85% of all lung cancer cases. The transcriptional factor brachyury has been verified to promote tumor cells migrate, invade, and metastasis in various types of tumors, whereas divergent roles of brachyury on cell proliferation have been reported in several types of tumor cells. In this study, we attempted to explore the effect of brachyury on the cell cycle progression and proliferation capability of NSCLC cells. Firstly, we performed RNA-sequence and ChIP-sequence to explore underlying downstream pathways regulated by brachyury. Cell proliferation and colony formation assays were utilized to detect the effect of brachyury on the proliferation ability of two types of lung NSCLC cells: H460 and Calu-1, which represent different brachyury expression levels. Following cell cycle and cell apoptosis assays were used to investigate the mechanism by which brachyury promotes NSCLC grow and progression. RNAsequence and ChIP-sequence (ChIP-seq) showed that one of the vital downstream pathways regulated by brachyury involves in cell cycle progression. Through cell proliferation assays and colony formation assays, we found that inhibition of brachyury could decrease the capability of proliferation in H460 cells. We also found that brachyury overexpression could prevent the transition from G0/G1 to S phase in Calu-1 cells, and brachyury knockdown could decrease the transition of G2/M phase in H460 cells. The cell apoptosis assays showed that inhibition of brachyury could promote apoptosis in H460 cells. In this study we demonstrate that brachyury and downstream target genes together involve in tumor cell cycle regulation by inducing accelerated transition through G2/M, promote tumor cell proliferation and inhibit apoptosis in lung NSCLC H460 cells. Targeting brachyury expression could be developed into a promising avenue for the prevention of lung cancer progression.

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CircFLNA facilitates gastric cancer cell proliferation and glycolysis via regulating SOX5 by sponging miR-1200

Yang,

Li,

Sun

et al. 2024

Arab Journal of Gastroenterology

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Transactivation of SOX5 by Brachyury promotes breast cancer bone metastasis

Cited by 21 publications

References 46 publications

The Roles of Embryonic Transcription Factor BRACHYURY in Tumorigenesis and Progression

The Roles of Embryonic Transcription Factor BRACHYURY in Tumorigenesis and Progression

The Role of Transcriptional Factor Brachyury on Cell Cycle Regulation in Non-small Cell Lung Cancer

CircFLNA facilitates gastric cancer cell proliferation and glycolysis via regulating SOX5 by sponging miR-1200

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