Transaminases in the Epididymal Fluid of the Ram

Alumot, Eugenia; Schindler, H

doi:10.1530/jrf.0.0100261

Cited by 3 publications

(3 citation statements)

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“…The fact that the activity of got in the epididymal fluid is several times higher than that found in the blood, points to the possible existence of a transaminase mechanism in the epididymal fluid, independent of that in the blood. This phenomenon has been reported previously in epididymal fluid of the ram (Alumot & Schindler, 1965). The possibility of leakage from spermatozoa and/or epithelial cells of the epididymis should be taken into account in any hypothesis designed to explain these observations.…”

supporting

confidence: 88%

Lactic Dehydrogenase and Transaminases in Bovine Epididymal Fluid

Stallcup¹,

Brown²,

Cole³

1968

Reproduction

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The activity of the enzyme lactic dehydrogenase (ld) in bovine blood serum (Roussel & Stallcup, 1965a) and in semen (Roussel & Stallcup, 1965c), and of glutamic-oxalacetic transaminase (got) and glutamic-pyruvic transaminase (gpt) in bovine blood serum (Roussel & Stallcup, 1965b) and semen have been previously reported and the literature reviewed (Flipse, 1960; Gregoire, Rakoff & Ward, 1961; Roussel & Stallcup, 1965d). The activity of the transaminases in the epididymal fluid of the ram has been reported by Alumot & Schindler (1965). The experiments reported here were undertaken to measure the activities of ld, got and gpt in bovine epididymal fluids compared to their activity in blood serum. Eight Aberdeen-Angus bulls, 14 months of age, were used in these studies. All animals were from the herds of the Arkansas Agricultural Experiment Station, and a complete life history as to age, diet and management practices was available in connection with research concerned with animal breeding. Blood samples were obtained from the jugular vein of each bull 1 week before slaughter, transferred to screw cap vials, and cooled; after coagulation they

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confidence: 88%

Lactic Dehydrogenase and Transaminases in Bovine Epididymal Fluid

Stallcup¹,

Brown²,

Cole³

1968

Reproduction

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“…A similar conclusion can be drawn from the study on transaminases in the epididymal fluid and seminal plasma of the ram. The activity of one of the enzymes (GOT) was found to exceed many times that of the blood, whereas another enzyme (GPT) was not active, despite its presence in the blood (Alumot & Schindler, 1965).…”

Section: Discussionmentioning

confidence: 96%

Separation of Proteins in the Epididymal Fluid of the Ram

Alumot¹,

Lensky²,

Schindler³

1971

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Proteins of ram epididymal fluid, seminal plasma, accessory gland fluid and blood serum were fractionated by electrophoresis. Nineteen bands of different mobilities were observed by disc electrophoresis. Immunoelectrophoresis revealed sixteen precipitation arcs in epididymal fluid and seminal plasma and fourteen in blood serum, following absorption with the antiserum against epididymal fluid. These results indicate that most of the epididymal fluid and seminal plasma proteins of the ram have antigens in common with blood serum.

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“…Aspartate aminotransferase has been found in ram seminal plasma. It occurs to some extent in epididymal fluid (Alumot and Schindler 1965;Murdoch and White 1968) and its activity may also be partly responsible for the high concentration of aspartic acid in ram seminal plasma.…”

Section: Incubation Of Semenmentioning

confidence: 99%

Amino Acid Composition of Semen and the Secretions of the Male Reproductive Tract

Pd¹,

White²

1974

Aust. Jnl. Of Bio. Sci.

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The free amino acid content of rete testis fluid, epididymal fluid, vesicular fluid and ejaculated seminal plasma was studied in the ram, bull and rat, and a comparison made with the blood plasma of the same species. The amino acid content of human seminal plasma and the various fractions of the boar ejaculate was also determined.Glutamic acid was found to be the predominant amino acid in the secretions of the reproductive tract of the ram and bull. It is present in high concentrations in the rete testis fluid of both species, and is further concentrated along the tract. In the rat glycine and alanine are the predominant amino acids in the rete testis fluid, while glutamic acid is the principal amino acid in epididymal fluid, as in the other species studied. Glycine is also the principal amino acid in human seminal plasma. The concentration of amino acids is greatest in the sperm-rich fraction of the boar ejaculate.The breakdown of protein to amino acid was studied in ram and bull semen. This is at least partly due to enzymically induced changes.

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Transaminases in the Epididymal Fluid of the Ram

Cited by 3 publications

References 1 publication

Lactic Dehydrogenase and Transaminases in Bovine Epididymal Fluid

Lactic Dehydrogenase and Transaminases in Bovine Epididymal Fluid

Separation of Proteins in the Epididymal Fluid of the Ram

Amino Acid Composition of Semen and the Secretions of the Male Reproductive Tract

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