Transciptome Analysis Molecular Mechanism of Starch Synthesis During Tuber Development in Chinese Yam <i>(Dioscorea opposita)</i>

Zhang, Yanfang; Guo, Sheng; Shao, Ying; Zhao, Lingmin; Xing, Linan; Ge, Mingran; Huo, Xiuwen

doi:10.1166/jbmb.2021.2103

Cited by 4 publications

(1 citation statement)

References 24 publications

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“…The gene BAM1 (Unigene0000684) was screened according to the transcriptome results obtained for yam via our previous high-throughput sequencing study [ 37 , 38 ]. Total RNA was isolated from tuber using a MiniBEST Plant RNA Extraction Kit (Takara, Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

Overexpression of DoBAM1 from Yam (Dioscorea opposita Thunb.) Enhances Cold Tolerance in Transgenic Tobacco

Zhao

Zhang

Shao

et al. 2022

Genes

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β-amylase (BAM) plays an important role in plant development and response to abiotic stresses. In this study, 5 DoBAM members were identified in yam (Dioscorea opposita Thunb.). A novel β-amylase gene BAM1, (named DoBAM1), was isolated from yam varieties Bikeqi and Dahechangyu. The open reading frame (ORF) of DoBAM1 is 2806 bp and encodes 543 amino acids. Subcellular localization analysis indicates that DoBAM1 localizes to the cell membrane and cytoplasm. In the yam variety Dahechangyu, the starch content, β-amylase activity, and expression of DoBAM1 were characterized and found to all be higher than in Bikeqi. DoBAM1 overexpression in tobacco is shown to promote the accumulation of soluble sugar and chlorophyll content and to increase the activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), and β-amylase. Under cold treatment, we observed the induced upregulation of DoBAM1 and lower starch content and malondialdehyde (MDA) accumulation than in WT plants. In conclusion, these results demonstrate that DoBAM1 overexpression plays an advanced role in cold tolerance, at least in part by raising the levels of soluble sugars that are capable of acting as osmolytes or antioxidants.

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Section: Methodsmentioning

confidence: 99%

Overexpression of DoBAM1 from Yam (Dioscorea opposita Thunb.) Enhances Cold Tolerance in Transgenic Tobacco

Zhao

Zhang

Shao

et al. 2022

Genes

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Transcriptome Analysis Reveals the Accumulation Mechanism of Starch in Chinese Yam (Dioscorea opposite Thunb.) Tubers Using RNA-Seq and Iso-Seq

et al. 2022

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The starch composition of tubers affects the quality and yield of yam, and it is of great significance to deeply investigate the mechanism of starch accumulation in yam. In this study, two yam varieties with significant differences in starch content, Bikeqi (Dioscorea opposita Bikeqi) and Dahechangyv (Dioscorea opposita Dahechangyu), were used as materials. The transcriptome of tubers from seven developmental stages were sequenced using RNA-seq and Iso-seq. Differential genes closely contacted with the metabolism of starch and sucrose were screened and the functions were analysed. The results showed that, the starch content of the two yam varieties reached the maximum at 150 and 165 days respectively, and their difference was the largest at 150 days. Total 21623 unigenes were annotated, and 8378 differential genes were identified within varieties by comparing each developmental stage with the beginning of tuber expansion (90 days), respectively. A total of 5177 differential genes were identified in two varieties in the same period. GO and KEGG were used to analyze these differential genes, a variety of key genes closely related to starch and sucrose metabolism were screened. These results help us to better understand the mechanism of starch accumulation in yam.

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Physicochemical Properties, Structural and In Vitro Digestibility of Starch from Five Different Sources

Wang

2022

sci adv mater

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Starch is one of the main sources of carbon water in food, the digestion rates of starches in food directly affects the concentration of glucose in the body. There are few comparative studies on the structures and digestibility starches from different sources. In this study, the molecular composition, physicochemical properties, crystal structure and digestion characteristics of five different sources of starches from purple yam (Dioscorea alata L.), iron stick yam (Dioscorea opposita Thunb. Cv. Tiegun) (DTT), potato (Solanum tuberosum L.), wheat (Triticum aestivum L.) and pea (Pisum sativum L.) were analyzed. The results showed that the content of resistant starches in DTT and purple yam were 84.1 and 73%, respectively, much higher than that of starches in potato (57.87%), pea (47.6%) and wheat (46.09%). The relative crystallinity of starch in purple yam was 45.5%, higher than the other four starches, in which that in wheat to be the lowest (33.1%). Starches in Purple yam and DTT contain more amylopectin medium chains and higher gelatinization temperature, while potato starch has the highest peak viscosity (PV). In general, purple yam and DTT starch have higher ordered chains and low digestibility, especially purple yam starch. The higher ordered chains is the main factor for the lower starch digestibility. This paper aims to reveal the relationship between starch structure and digestibility, and is expected to provide a reference for a better development and application of starch functional foods.

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Transciptome Analysis Molecular Mechanism of Starch Synthesis During Tuber Development in Chinese Yam (Dioscorea opposita)

Cited by 4 publications

References 24 publications

Overexpression of DoBAM1 from Yam (Dioscorea opposita Thunb.) Enhances Cold Tolerance in Transgenic Tobacco

Overexpression of DoBAM1 from Yam (Dioscorea opposita Thunb.) Enhances Cold Tolerance in Transgenic Tobacco

Transcriptome Analysis Reveals the Accumulation Mechanism of Starch in Chinese Yam (Dioscorea opposite Thunb.) Tubers Using RNA-Seq and Iso-Seq

Physicochemical Properties, Structural and In Vitro Digestibility of Starch from Five Different Sources

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