Transcript Profiling of Hevea brasiliensis during Latex Flow

Chao, Jinquan; Yang, Shuguang; Chen, Yueyi; Weimin, Tian

doi:10.3389/fpls.2017.01904

Cited by 8 publications

(4 citation statements)

References 42 publications

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“…The secondary laticifers located in the bark of the trunk of H. brasiliensis are the site closely related to natural rubber production (Hao and Wu, 2000). Latex, a milky cytoplasm of laticifers, contains 20–40% of the rubber and is used as the raw material for refining natural rubber (Chao et al, 2017; Zhang et al, 2017). Although application of ethrel (an ethylene releaser) causes a significant increase in rubber yield per tapping via prolonging the duration of latex flow (Zhu and Zhang, 2009), it inhibits rubber biosynthesis (Deng et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Characterization of the JAZ Gene Family in Rubber Tree (Hevea brasiliensis)

et al. 2019

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Jasmonate signaling plays a vital role in the regulation of secondary laticifer differentiation and natural rubber biosynthesis in Hevea brasiliensis . Jasmonate ZIM-domain (JAZ) proteins are the master regulators of jasmonate signaling. Although several JAZs have been reported in the laticifer cells of H. brasiliensis , the genome-wide screening of HbJAZ members has not yet been explored. In the present study, 18 HbJAZs were identified based on the recent H. brasiliensis genome. Phylogenetic construction revealed that the HbJAZs were clustered into five subgroups and that members within the same subgroup shared highly conserved gene structures and protein motifs. Cis -element analysis of HbJAZ promoters suggested the presence of hormone, stress and development-related cis -elements. HbJAZ1.0, HbJAZ2.0, and HbJAZ5.0 interacted with CORONATINE INSENSITIVE1 (COI1) in the presence of coronatine (COR, a JA mimic). HbJAZ1.0, HbJAZ2.0, HbJAZ5.0, and HbJAZ12.0 could also interact with each other. Of the 18 HbJAZs , transcripts of 15 HbJAZs were present in the vascular cambium region except for that of HbJAZ7.0 , HbJAZ8.0d , and HbJAZ13.0 . Fourteen of the 15 HbJAZs were significantly up-regulated upon COR treatment. The transcripts of three genes that were absent from vascular cambium region were also absent from the latex. Among the 15 HbJAZs in the latex, the expression patterns of 13 HbJAZs were different between the tapping and ethrel treatments. Eight of the 14 COR-up-regulated HbJAZs in the vascular cambium region were also activated by tapping in latex. Of the eight tapping-activated HbJAZs , 5 HbJAZs were repressed by ethrel application. Based on the computational analyses and gene expression patterns described in this study, the HbJAZ5.0 and HbJAZ10.0b may be associated with laticifer differentiation while the HbJAZ8.0b is a negative regulator for natural rubber biosynthesis in H. brasiliensis .

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Characterization of the JAZ Gene Family in Rubber Tree (Hevea brasiliensis)

et al. 2019

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“…In rubber trees (Hevea brasiliensis), natural rubber synthesis is the main metabolic activity in laticifers which are highly specialized cells that directly influence the yield of rubber [ 19]. Most studies in molecular biology, especially those using gene expression profiling, have paid close attention to rubber biosynthesis in laticifers [20][21][22][23][24][25]. Previously, we have evaluated the expression stability of 22 reference genes across abundant laticifer samples, including latex regenerations, hormone treatments, tappings, individual trees and genotypes of Hevea [ 26,27].…”

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Identification and evaluation of suitable reference genes for gene expression analysis in rubber tree leaf

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Kav

et al. 2020

Mol Biol Rep

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Backgroud Gene expression profiling is increasingly applied to investigate molecular mechanisms for which, normalization with suitable reference genes is critical. Previously we have reported several suitable reference genes for laticifer samples from rubber, however, little is known about reference genes in leaf. Results The main objective of this current study was to identify some reference genes with stable expression patterns in leaf at various developmental stages, as well as during abiotic (temperature extremes) and biotic stresses. Gene expression profiling experiments in rubber tree leaf identified the ubiquitinproteasome system as having excellent potential as reference genes. Among a total of 30 tested genes investigated, 24 new (including 11 genes involved in the ubiquitin-proteasome system), 4 previously identified and 2 specific genes, were further evaluated using quantitative real-time PCR. Our results indicated that the new genes had better stability of expression when compared with others. For instance, an ubiquitin conjugating enzyme (RG0099) and three ubiquitin-protein ligases (RG0928, RG2190 and RG0118) expressed stably in all samples, and were confirmed to be suitable reference genes in rubber tree leaf in four different conditions. Finally, we suggest that using more than one reference gene may be appropriate in gene expression studies when employing different software to normalize gene expression data. Conclusion Our findings have significant implications for the reliability of data obtained from genomics studies in rubber tree and perhaps in other species. Background Gene expression profiling studies are widely used to investigate plant processes, including metabolic pathways, including regulatory networks that are crucial for plant growth & development as well as in stress responses. These types of genomics-based investigation necessitate the use of appropriate reference genes to normalize gene expression data. Incorrect choice of reference genes in techniques

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“…The rubber tree is the world's main source of natural rubber (cis-1, 4-polyisoprene) [11]. As an important industrial raw material, natural rubber is synthesized by the laticifers located in the inner bark of the rubber tree [12]. The milky laticifer cytoplasm, named latex, contains 20%-40% natural rubber.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and expression analysis of the phosphatase 2A family in rubber tree (Hevea brasiliensis)

et al. 2020

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The protein phosphatase 2As (PP2As) play a key role in manipulating protein phosphorylation. Although a number of proteins in the latex of laticifers are phosphorylated during latex regeneration in rubber tree, information about the PP2A family is limited. In the present study, 36 members of the HbPP2A family were genome-wide identified. They were clustered into five subgroups: the subgroup HbPP2AA (4), HbPP2AB' (14), HbPP2AB'' (6), HbPP2AB55 (4), and HbPP2AC (8). The members within the same subgroup shared highly conserved gene structures and protein motifs. Most of HbPP2As possessed ethylene-and wounding-responsive cis-acting elements. The transcripts of 29 genes could be detected in latex by using published high-throughput sequencing data. Of the 29 genes, seventeen genes were significantly down-regulated while HbPP2AA1-1 and HbPP2AB55α/Bα-1were up-regulated by tapping. Of the 17 genes, 14 genes were further significantly down-regulated by ethrel application. The down-regulated expression of a large number of HbPP2As may attribute to the enhanced phosphorylation of the proteins in latex from the tapped trees and the trees treated with ethrel application.

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Transcript Profiling of Hevea brasiliensis during Latex Flow

Cited by 8 publications

References 42 publications

Genome-Wide Identification and Characterization of the JAZ Gene Family in Rubber Tree (Hevea brasiliensis)

Genome-Wide Identification and Characterization of the JAZ Gene Family in Rubber Tree (Hevea brasiliensis)

Identification and evaluation of suitable reference genes for gene expression analysis in rubber tree leaf

Genome-wide identification and expression analysis of the phosphatase 2A family in rubber tree (Hevea brasiliensis)

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